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  4. GST Monoclonal Antibody

GST Monoclonal Antibody

  • 货号：
    CSB-MA000031M1m
  • 规格：
    ￥400
  • 图片：
    • Western Blot
      Positive WB detected in: 25ng Recombinant protein with GST tag from E.coli
      All lanes: GST antibody at 1:10000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 32.6, 30.1, 29.5, 37.4, 30.4, 60.5, 38.1, 61.6 KDa
      Observed band size: 31, 30, 31, 37, 30, 60, 37, 61 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with GST tag from E.coli
      All lanes: GST antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 26, 102.9, 75.6 KDa
      Observed band size: 26, 103, 76 KDa
      Exposure time：1min
    • Western Blot
      Positive WB detected in: Recombinant protein with GST tag from E.coli at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
      All lanes: GST antibody at 1:10000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 26 KDa
      Observed band size: 26 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: 25ng recombinant protein with GST tag from E.coli
      GST antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 26 KDa
      Observed band size: 26 KDa
      Exposure time：5min
    • Immunofluorescence staining of 293F transfected cells with CSB-MA000031M1m at 1:94.5, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
    • Immunoprecipitating GST in transfected-293F whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA000031M1m in 293F cell lysate Transfected with GST
      Lane 2: CSB-MA000031M1m (5µg) + Transfected-293F whole cell lysate (500µg)
      Lane 3: Transfected-293F whole cell lysate (10µg)
      For western blotting, the blot was detected with CSB-MA000031M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
    • Overlay Peak curve showing 293F cells transfected with GST stained with CSB-MA000031M1m (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    The glutathione S transferase (GST) monoclonal antibody is secreted from the hybridoma formed by the fusion of mouse myeloma cells and splenocytes from mice immunized with the recombinant GST protein. It is purified from mouse ascites through protein A, and its purity reaches over 95%. This unconjugated GST monoclonal antibody is matched with the mouse IgG2b isotype. It is recommended for use in several scientific applications, including ELISA, WB, IF, FC, and IP applications, and can detect the GST tag from all species.

    GST is a 26 kDa protein that naturally occurs in eukaryotic cells. GSTs can catalyze the binding of exogenous substances to glutathione (GSH), thereby detoxifying many environmental toxins including chemotherapy drugs, residual drugs, herbicides and carcinogens. Many researchers integrate GST DNA sequences into expression vectors to produce fusion proteins with GST tags. GST can quickly fold and be stable and has good solubility, so it can promote the expression and dissolution of the fusion protein. In addition, the binding between GST and its substrate GSH can also be used for protein purification and detection. At present, GST-tagged proteins are used in recombinant protein expression, affinity purification, antigen immunity, vaccine research, and pull-down, affinity chromatography and ELISA in a variety of research technology experiments.

  • 基因名：
    GST
  • 别名：
    Glutathione S-Transferase, gst, GST tag
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    Recombinant GST Protein
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    3D3E4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, FC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:10000-1:640000
    IF 1:100-1:300
    FC 1:100-1:300
    IP 1ul-5ul
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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