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His Monoclonal Antibody

  • 货号：
    CSB-MA000011M1m
  • 规格：
    ￥400
  • 图片：
    • Western Blot
      Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 43.4 KDa
      Observed band size: 43.4 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size:62.5 KDa
      Observed band size: 66 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in:200ng of four Recombinant protein with his tag All lanes: His antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 43.4, 62.5,56,80.6 KDa
      Observed band size:43.4, 66, 57 ,90 KDa
      Exposure time：5min
    • Immunoprecipitating His in 293 whole cell lysate transfected with His
      Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His. Lane 2: 2F4B5 antibody (2µg) + 293 whole cell lysate transfected with His (500µg)
      Lane 3: 293 whole cell lysate transfected with His (5µg)
      For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
  • 其他：

产品详情

  • 产品描述：
    The His Monoclonal Antibody specifically reacts to recombinant proteins containing the His-Tag. The His tag is widely used in recombinant protein isolation and purification, and this antibody is a valuable tool for researchers who need to detect or isolate proteins that have been tagged with the His sequence.
     
    The His Monoclonal Antibody underwent protein G  mediated purification and was purified to a high degree of purity, >95%. It is available in liquid form and belongs to the IgG1 isotype. This antibody has been validated in ELISA, WB and IP assays, making it a useful tool for the detection and quantification of recombinant proteins containing the His-Tag. Its high specificity and purity ensure reliable and reproducible results.
  • 别名：
    6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    The immunogen is a mixture of various His-tagged proteins
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    2F4B5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:640000
    IP 2µl-5µl
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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