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  4. V5-Tag Monoclonal Antibody

V5-Tag Monoclonal Antibody

  • 货号：
    CSB-MA000161M0m
  • 规格：
    ￥400
  • 图片：
    • Western Blot
      Positive WB detected in: 50ng V5-tagged fusion protein
      V5 Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 37.8 KDa
      Observed band size: 43 KDa
      Exposure time: 10S
    • Western Blot
      Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:5000
      Lane 1: Recombinant V5-tagged fusion protein at 50ng
      Lane 2: Recombinant V5-tagged fusion protein at 25ng
      Lane 3: Recombinant V5-tagged fusion protein at 12.25ng
      Lane 4: Recombinant V5-tagged fusion protein at 6.25ng
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 37.8 kd
      Observed band size: 43 kd
      Exposure time: 5min
    • Western Blot
      Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4 at 50ng
      All lanes: V5 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 50.2, 47.6, 66.7, 37.8 KDa
      Observed band size: 52, 47, 67, 42 KDa
      Exposure time: 30s
    • Western Blot
      Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, at 50ng
      All lanes: V5 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 31.9, 20.4, 24.2, 53.4 KDa
      Observed band size: 32, 21, 28, 58 KDa
      Exposure time: 5min
    • Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).
    • Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
      Lane 1: Mouse control IgG1 instead of CSB-MA000161M0m in Transfected HEK293F cells whole cell lysate
      Lane 2: CSB-MA000161M0m (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg)
      Lane 3: Transfected HEK293F cells whole cell lysate (20µg)
      For western blotting, the blot was detected with CSB-MA000161M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
    • Overlay histogram showing untransfected HEK293F cells (green line) or transfected HEK293F cells (red line) stained with CSB-MA000161M0m. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    The V5-Tag monoclonal antibody is generated using recombinant the GKPIPNPLLGLDST synthetic peptide conjugate to KLH as the immunogen, with B cells from immunized mice being fused with myeloma cells to create hybridomas. The hybridoma cells that produce the V5-Tag antibody are screened and cultured, and the V5-Tag monoclonal antibody is purified from the mouse ascites using protein G affinity chromatography, with a purity of over 95%. This unconjugated IgG1 antibody is suitable for detecting all V5-Tagged proteins in ELISA, WB, IF, IP, and FC assays.

    The V5-tag is a short peptide sequence (GKPIPNPLLGLDST) that is commonly added to recombinant proteins to facilitate their detection and purification. It is often fused to a protein of interest using genetic engineering techniques. The V5-tag is widely used in the study of protein-protein interactions, protein localization, and protein trafficking within cells.

  • 基因名：
    V5-Tag
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    GKPIPNPLLGLDST synthetic peptide conjugate to KLH
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    8A7A8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:2000-1:80000
    IF 1:100-1:200
    IP 2µl-5µl
    FC 1:100-1:300
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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