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  4. Recombinant Bacillus subtilis Sensor histidine kinase desK (desK)

Recombinant Bacillus subtilis Sensor histidine kinase desK (desK)

  • 中文名称：
    枯草芽孢杆菌desK重组蛋白
  • 货号：
    CSB-CF516538BRJ
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    desK
  • Uniprot No.：
  • 别名：
    desK; yocF; BSU19190; Sensor histidine kinase DesK
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-370
  • 氨基酸序列
    MIKNHFTFQKLNGITPYIWTIFFILPFYFIWKSSSTFVIIVGIILTLLFFSVYRFAFVSK GWTIYLWGFLLIGISTASITLFSYIYFAFFIAYFIGNIKERVPFHILYYVHLISAAVAAN FSLVLKKEFFLTQIPFVVITLISAILLPFSIKSRKERERLEEKLEDANERIAELVKLEER QRIARDLHDTLGQKLSLIGLKSDLARKLIYKDPEQAARELKSVQQTARTSLNEVRKIVSS MKGIRLKDELINIKQILEAADIMFIYEEEKWPENISLLNENILSMCLKEAVTNVVKHSQA KTCRVDIQQLWKEVVITVSDDGTFKGEENSFSKGHGLLGMRERLEFANGSLHIDTENGTK LTMAIPNNSK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Member of the two-component regulatory system DesR/DesK, responsible for cold induction of the des gene coding for the Delta5 acyl-lipid desaturase. Acts as a sensor of the membrane fluidity. Probably activates DesR by phosphorylation.
  • 基因功能参考文献：
    1. The thickness of lipid membranes varies with temperature and that such variations can be detected by DesK with great precision. PMID: 27528507
    2. In vivo and biochemical experiments, structural analyses and simulations, demonstrated that stabilization/destabilization of a 2-helix coiled coil is directly involved in regulating DesK's functional state. PMID: 26172072
    3. A lipid-mediated conformational switch modulates the thermosensing activity of DesK. PMID: 24522108
    4. Data show that the membrane-bound histidine kinase DesK phosphorylates the response regulator DesR to activate des transcription. PMID: 19595746
    5. Data from the structural comparison of the different DesK variants indicates that incoming signals can take the form of helix rotations and asymmetric helical bends similar to those reported for other sensing systems. PMID: 19805278

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 数据库链接：

    KEGG: bsu:BSU19190

    STRING: 224308.Bsubs1_010100010591