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  4. Recombinant Bacillus subtilis Stage II sporulation protein SA (spoIISA)

Recombinant Bacillus subtilis Stage II sporulation protein SA (spoIISA)

  • 中文名称：
    枯草芽孢杆菌spoIISA重组蛋白
  • 货号：
    CSB-CF520274BRJ
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    spoIISA
  • Uniprot No.：
  • 别名：
    spoIISA; ykaC; BSU12830; Stage II sporulation protein SA; Killer protein SpoIISA; Toxin SpoIISA
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-248
  • 氨基酸序列
    MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSL FTHWERYLIVAVSFALIDAFIFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTY QYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASLCHYSTQADKDRLTEHMDDPA DVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL PIEEEGEG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Toxic component of a type II toxin-antitoxin (TA) system. Its toxic activity is neutralized by cognate antitoxin SpoIISB. Expression in the absence of SpoIISB permits sporulation to stage II, when plasmolysis zones and holes in the peptidoglycan layer are observed, resulting in cell death. Lethal when synthesized during vegetative growth in the absence of SpoIISB. In E.coli both the membrane bound and soluble domain are required in cis for toxin activity.
  • 基因功能参考文献：
    1. Using truncated SpoIISA constructs, s show that the entire transmembrane domain is required for its toxicity. PMID: 25039482
    2. SpoIISA levels increase in stationary phase and localized to cytoplasmic membrane. PMID: 20863891
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SpoIISA toxin family
  • 数据库链接：

    KEGG: bsu:BSU12830

    STRING: 224308.Bsubs1_010100007111