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  4. Recombinant Bovine cGMP-gated cation channel alpha-1 (CNGA1)

Recombinant Bovine cGMP-gated cation channel alpha-1 (CNGA1)

  • 货号：
    CSB-CF005642BO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    CNGA1; CNCG; CNCG1; cGMP-gated cation channel alpha-1; Cyclic nucleotide-gated cation channel 1; Cyclic nucleotide-gated channel alpha-1; CNG channel alpha-1; CNG-1; CNG1; Cyclic nucleotide-gated channel, photoreceptor; Rod photoreceptor cGMP-gated channel subunit alpha
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-690
  • 氨基酸序列
    MKKVIINTWHSFVNIPNVVGPDVEKEITRMENGACSSFSGDDDDSASMFEESETENPHAR DSFRSNTHGSGQPSQREQYLPGAIALFNVNNSSNKEQEPKEKKKKKKEKKSKPDDKNENK KDPEKKKKKEKDKDKKKKEEKGKDKKEEEKKEVVVIDPSGNTYYNWLFCITLPVMYNWTM IIARACFDELQSDYLEYWLAFDYLSDVVYLLDMFVRTRTGYLEQGLLVKEERKLIDKYKS TFQFKLDVLSVIPTDLLYIKFGWNYPEIRLNRLLRISRMFEFFQRTETRTNYPNIFRISN LVMYIIIIIHWNACVYFSISKAIGFGNDTWVYPDVNDPDFGRLARKYVYSLYWSTLTLTT IGETPPPVRDSEYFFVVADFLIGVLIFATIVGNIGSMISNMNAARAEFQARIDAIKQYMH FRNVSKDMEKRVIKWFDYLWTNKKTVDEREVLKYLPDKLRAEIAINVHLDTLKKVRIFAD CEAGLLVELVLKLQPQVYSPGDYICKKGDIGREMYIIKEGKLAVVADDGITQFVVLSDGS YFGEISILNIKGSKAGNRRTANIKSIGYSDLFCLSKDDLMEALTEYPDAKGMLEEKGKQI LMKDGLLDINIANAGSDPKDLEEKVTRMESSVDLLQTRFARILAEYESMQQKLKQRLTKV EKFLKPLIDTEFSAIEGSGTESGPTDSTQD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Subunit of the rod cyclic GMP-gated cation channel, which is involved in the final stage of the phototransduction pathway. When light hits rod photoreceptors, cGMP concentrations decrease causing rapid closure of CNGA1/CNGB1 channels and, therefore, hyperpolarization of the membrane potential.
  • 基因功能参考文献：
    1. These results suggest that restraining the A' helix to the plasma membrane potentiates CNGA1 channel opening. PMID: 26969165
    2. Conformation changes of CNGA1 associated with its gating function are reported. PMID: 25963832
    3. Data show that when Pro365 is replaced with a threonine in cyclic nucleotide gated channel alpha 1 (CNGA1), cesium Cs+ permeation is nearly abolished. PMID: 26100907
    4. Stabilizing the C-helix of intact CNGA1 channels by metal binding to a pair of histidines promoted channel opening. PMID: 23525108
    5. A ring of threonines in the inner vestibule of the pore of CNGA1 channels constitutes a binding site for permeating ions PMID: 22869010
    6. Three CNGA1 subunits and a single CNGB1 subunit form the rod cyclic nucleotide-gated ion channels. PMID: 21878911
    7. Existence of a strong coupling between the upper portion of the S6 helix provides a simple molecular mechanism for CNGA1 gating. PMID: 19289572
    8. The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichiometry PMID: 12432397
    9. Data suggest that the native rod cyclic nucleotide-gated (CNG) channel is a tetramer composed of three A1 and one B1 subunit. PMID: 12467591
    10. Data show that the cyclic nucleotide-gated (CNG) channel contains three CNGA1 subunits and only one CNGB1 subunit. PMID: 12467592
    11. Structural models of the tetrameric cyclic nucleotide gated channel alpha 1 from bovine rod is presented. PMID: 15792804
    12. In CNGA1 channels, pore gating is involved in the rate-limiting reactions determining the activation time course in the sequence of events between cGMP binding and pore opening. PMID: 16473910
    13. These observations suggest that these effects are mediated by the formation of a disulfide bond (S-S) between F380C and the endogenous Cys-314 in the S5 segment. PMID: 16513780
    14. analyze in detail the molecular mechanisms underlying Cd2+ inhibition and longer cross-linkers in mutant CNGA1 channels PMID: 19132361
    15. detailed analysis of conformational changes of the S6 domain and C-linker during gating of CNGA1 channels PMID: 19488745

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Cyclic nucleotide-gated cation channel (TC 1.A.1.5) family, CNGA1 subfamily
  • 组织特异性：
    Expressed in the retina, in rod cells (at protein level).
  • 数据库链接：

    KEGG: bta:281700

    STRING: 9913.ENSBTAP00000002853

    UniGene: Bt.53