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Recombinant Bovine viral diarrhea virus Genome polyprotein

  • 中文名称：
    Recombinant Bovine viral diarrhea virus Genome polyprotein
  • 货号：
    CSB-CF324843BKW
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Genome polyprotein
  • 种属：
    Bovine viral diarrhea virus (isolate NADL) (BVDV) (Mucosal disease virus)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    3270-3988
  • 氨基酸序列
    SSWFLKASNKQMSLTPLFEELLLRCPPATKSNKGHMASAYQLAQGNWEPLGCGVHLGTIP ARRVKIHPYEAYLKLKDFIEEEEKKPRVKDTVIREHNKWILKKIRFQGNLNTKKMLNPGK LSEQLDREGRKRNIYNHQIGTIMSSAGIRLEKLPIVRAQTDTKTFHEAIRDKIDKSENRQ NPELHNKLLEIFHTIAQPTLKHTYGEVTWEQLEAGVNRKGAAGFLEKKNIGEVLDSEKHL VEQLVRDLKAGRKIKYYETAIPKNEKRDVSDDWQAGDLVVEKRPRVIQYPEAKTRLAITK VMYNWVKQQPVVIPGYEGKTPLFNIFDKVRKEWDSFNEPVAVSFDTKAWDTQVTSKDLQL IGEIQKYYYKKEWHKFIDTITDHMTEVPVITADGEVYIRNGQRGSGQPDTSAGNSMLNVL TMMYGFCESTGVPYKSFNRVARIHVCGDDGFLITEKGLGLKFANKGMQILHEAGKPQKIT EGEKMKVAYRFEDIEFCSHTPVPVRWSDNTSSHMAGRDTAVILSKMATRLDSSGERGTTA YEKAVAFSFLLMYSWNPLVRRICLLVLSQQPETDPSKHATYYYKGDPIGAYKDVIGRNLS ELKRTGFEKLANLNLSLSTLGVWTKHTSKRIIQDCVAIGKEEGNWLVKPDRLISSKTGHL YIPDKGFTLQGKHYEQLQLRTETNPVMGVGTERYKLGPIVNLLLRRLKILLMTAVGVSS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation.; Packages viral RNA to form a viral nucleocapsid and thereby protects viral RNA. Plays also a role in transcription regulation. Protects the incoming virus against IFN-induced effectors.; Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans.; E1 and/or E2 are probably responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis.; E1 and/or E2 are probably responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis.; Plays an essential role in the virus replication cycle by acting as a viroporin. Forms ion conductive pores, which alters the cell permeability allowing the transport of ions and other small molecules. Forms a leader sequence to properly orient NS2 in the membrane.; Uncleaved NS2-3 is required for production of infectious virus.; Plays a role in the regulation of viral RNA replication.; Multifunctional protein that contains an N-terminal protease and a C-terminal helicase, playing essential roles in viral polyprotein processing and viral genome replication. The chymotrypsin-like serine protease activity utilizes NS4A as an essential cofactor and catalyzes the cleavage of the polyprotein leading to the release of NS4A, NS4B, NS5A, and NS5B. Interacts with NS5B to enhance RNA-dependent RNA polymerase activity.; Acts as a cofactor for the NS3 protease activity.; Induces a specific membrane alteration that serves as a scaffold for the virus replication complex.; Replicates the viral (+) and (-) genome.
  • 基因功能参考文献：
    1. Bovine viral diarrhea virus 1 infection of monocyte-derived dendritic cells was prevented by incubating the virus with anti-viral E2 antibodies or by pretreating the cells with recombinant E2 protein before virus contact, suggesting that the virus infects monocyte-derived dendritic cells through an E2-mediated mechanism. PMID: 27529119
    2. E2 acts as a host complement regulatory protein. PMID: 27038454
    3. The viral N-terminal protease interact with host proteins and are components of the ribonucleoprotein complex. PMID: 24965446
    4. E2 glycoproteins of both BVDV type 1 and type2 exhibit a comparable antigenic structure, but with type specific epitopes PMID: 23838147
    5. These findings reveal the existence of an alternative -1/+2 ORF in the N(pro)(1-19) region during the replication of bovine viral diarrhea virus and open a new avenue to understand the life cycle and pathogenesis of pestiviruses. PMID: 22079882
    6. The NS2 and NS3 are not essential for virion morphogenesis. PMID: 22031952
    7. The minimal residues of NIBP that interact with NS5A of bovine viral diarrhea virus were mapped as aa 597-623. PMID: 20444997
    8. BVDV NS3/NS3Delta50 induces apoptosis initiated by caspase-8 activation and subsequent cytochrome c release-dependent caspase-9 activation. PMID: 15720974
    9. These findings suggest dual mechanisms of pestivirus superinfection exclusion, one at the level of viral entry that requires viral glycoprotein E2 and a second at the level of viral RNA replication. PMID: 15731218
    10. the results of this study demonstrate a surprising flexibility within the palm region of a plus-strand RNA virus RNA dependent RNA polymerase, resulting in viral attenuation in vitro. PMID: 17098978
    11. These results suggest that the NS2-3 protein of ncp bovine viral diarrhea virus 1 is cleaved in vivo. PMID: 18557898
    12. This study demonstrated that authentic, enzymically active E(rns) produced in mammalian cells prevented a dsRNA-induced IFN response when present in the supernatant of bovine cells. [E(rns)] PMID: 18796719
    13. These experiments showed that a Y1338H substitution at residue 193 of NS2 resulted in the temperaturedependent attenuation of cytopathogenicity despite high levels of infectious virus production. PMID: 19776121
    14. These data suggest that the NS3/4A protein is responsible for regulating the levels of cellular apoptosis and provide new insights regarding the viral elements associated with cytopathogenic biotype pathogenesis. PMID: 19793904

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  • 亚细胞定位：
    [Capsid protein C]: Virion.; [E(rns) glycoprotein]: Host membrane; Peripheral membrane protein. Virion membrane; Peripheral membrane protein.; [Envelope glycoprotein E2]: Host cell surface. Virion membrane.; [Cysteine protease NS2]: Host membrane; Multi-pass membrane protein.; [Serine protease NS3]: Host cytoplasm.; [Non-structural protein 4B]: Host cytoplasm.; [Non-structural protein 5A]: Host cytoplasm.
  • 蛋白家族：
    Pestivirus polyprotein family
  • 数据库链接：

    KEGG: vg:1489735