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  4. Recombinant Drosophila melanogaster Frizzled (fz)

Recombinant Drosophila melanogaster Frizzled (fz)

  • 中文名称：
    黑腹果蝇fz重组蛋白
  • 货号：
    CSB-CF325586DLU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    fz
  • Uniprot No.：
  • 别名：
    fz; CG17697; Frizzled; Frizzled-1; dFz1
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-581
  • 氨基酸序列
    YDQSPLDASPYYRSGGGLMASSGTELDGLPHHNRCEPITISICKNIPYNMTIMPNLIGHT KQEEAGLEVHQFAPLVKIGCSDDLQLFLCSLYVPVCTILERPIPPCRSLCESARVCEKLM KTYNFNWPENLECSKFPVHGGEDLCVAENTTSSASTAATPTRSVAKVTTRKHQTGVESPH RNIGFVCPVQLKTPLGMGYELKVGGKDLHDCGAPCHAMFFPERERTVLRYWVGSWAAVCV ASCLFTVLTFLIDSSRFRYPERAIVFLAVCYLVVGCAYVAGLGAGDSVSCREPFPPPVKL GRLQMMSTITQGHRQTTSCTVLFMALYFCCMAAFAWWSCLAFAWFLAAGLKWGHEAIENK SHLFHLVAWAVPALQTISVLALAKVEGDILSGVCFVGQLDTHSLGAFLILPLCIYLSIGA LFLLAGFISLFRIRTVMKTDGKRTDKLERLMLRIGFFSGLFILPAVGLLGCLFYEYYNFD EWMIQWHRDICKPFSIPCPAARAPGSPEARPIFQIFMVKYLCSMLVGVTSSVWLYSSKTM VSWRNFVERLQGKEPRTRAQAYV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Required to coordinate the cytoskeletons of epidermal cells to produce a parallel array of cuticular hairs and bristles.
  • 基因功能参考文献：
    1. The stabilizing function of Prickle on Frizzled requires Prickle activity in neighboring cells. Conversely, Prickle in the same cell has a destabilizing effect on Frizzled. PMID: 28918952
    2. instructive input of Frizzled-Planar cell polarity (Fz/PCP) signalling into polarized centriole positioning in Drosophila wings, is reported. PMID: 27021213
    3. Studies indicate that Frizzled (Fz) is constitutively ubiquitinated and internalized to the early endosome. PMID: 22825661
    4. We found no evidence for an essential requirement of the Fz C-terminal tail downstream of the KTxxxW motif in planar polarity and canonical Wnt signaling. PMID: 23023003
    5. Dissecting the molecular bridges that mediate the function of Frizzled in planar cell polarity. PMID: 22949620
    6. Frazzled is expressed and cell autonomously required in R8 photoreceptors for directing their axons to the medulla-neuropil layer M3. PMID: 22794263
    7. Frizzled and Dishevelled are enriched at distal sides of each cell and hence localize at the interface with ligand-expressing cells in the non-responding cells. PMID: 22736244
    8. Correct specification of a single R3 cell per ommatidium is essential for the normal interpretation of the Fz-mediated polarity signal. PMID: 9651479
    9. Frizzled is involved in positional information signaling during development in the coordinated orientation of ommatidia in the Drosophila eye. PMID: 11893338
    10. Function is dependent on inturned protein and on fuzzy protein. PMID: 11973308
    11. Pk causes fz to have an asymmetric localization in the cytoplasm of Drosophila. PMID: 12015986
    12. role of dachsous and fat acting upstream of frizzled in regulation of ommatidia orientation PMID: 12062069
    13. Genetic interactions and epistasis analysis suggest that frizzled, dishevelled,and fat act together in the long-range propagation of polarity signals in the eye and wing. PMID: 12479810
    14. Data show that Dishevelled (Dvl) binding peptides of Frizzled inhibited canonical Wnt signaling and blocked Wnt-induced secondary axis formation in a dose-dependent manner, but did not block noncanonical Wnt signaling. PMID: 14636582
    15. We propose a model whereby the dPatj/aPKC complex regulates PCP by inhibiting Fz1 in cells where it should not be active. PMID: 15907474
    16. Frizzled signalling is promoted by binding of Diego with Dishevelled. PMID: 15937478
    17. Drosophila frizzled controls planar cell polarity and Armadillo signaling by a common mechanism. PMID: 16085697
    18. Fz activates signaling in two steps: Fz uses its cysteine-rich domain to capture Wingless, and once bound Wg interacts with the membrane portion of the receptor to initiate signaling. PMID: 16163385
    19. wing cells acquire planar polarity along the proximal-distal axis, and Frizzled localizes at the distal side PMID: 16459300
    20. Drosophila planar cell polarity is under the hierarchical control of Frizzled (Fz)-a serpentine receptor (that also functions in the Wnt signaling pathway) [review] PMID: 17102631
    21. examined the role of a Dsh-binding PCP molecule, Diego (Dgo), in both Wnt-Fz/beta-catenin and Fz/PCP signaling PMID: 18065209

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor Fz/Smo family
  • 数据库链接：

    KEGG: dme:Dmel_CG17697

    STRING: 7227.FBpp0303135

    UniGene: Dm.1456