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  4. Recombinant Escherichia coli Glycerol uptake facilitator protein (glpF)

Recombinant Escherichia coli Glycerol uptake facilitator protein (glpF)

  • 中文名称：
    大肠杆菌glpF重组蛋白
  • 货号：
    CSB-CF365183ENV
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    glpF
  • Uniprot No.：
  • 别名：
    glpF; b3927; JW3898; Glycerol uptake facilitator protein; Aquaglyceroporin
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-281
  • 氨基酸序列
    MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAALVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILMGLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAGWGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSEQKASL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.
  • 基因功能参考文献：
    1. Mutation of tryptophan Trp219 affects the aqualgyeroporin GlpF activity and the stability of the active GlpF tetramer. PMID: 29069569
    2. Osmotic water permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations have been presented. PMID: 28455098
    3. Folding of the aquaglyceroporin GlpF determines its stability. (Review) PMID: 25462169
    4. TMAO increases the heat stability of the GF tetramer an average of 10 degrees C in the 4 detergents and also protects the protein from denaturation by SDS. PMID: 25387032
    5. The tetrameric alpha-helical GlpF unfolds via a dimeric folding intermediate. PMID: 22035256
    6. GlpF is found to be highly permeable for small solutes and permeable for larger solutes. PMID: 18202181
    7. GlpF exists as a stably folded tetramer in detergent solutions of neutral dodecyl maltoside and in zwitterionic lysomyristoylphosphatidylcholine. PMID: 18284214

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  • 亚细胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family
  • 数据库链接：

    KEGG: ecj:JW3898

    STRING: 316385.ECDH10B_4116