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  4. Recombinant Human E3 ubiquitin-protein ligase MARCH8 (41341)

Recombinant Human E3 ubiquitin-protein ligase MARCH8 (41341)

  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase MARCH8(41341)
  • 货号：
    CSB-CF722561HU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    MARCH8
  • Uniprot No.：
  • 别名：
    MARCHF8; MARCH8; MIR; RNF178; E3 ubiquitin-protein ligase MARCHF8; Cellular modulator of immune recognition; c-MIR; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; MARCH-VIII; RING finger protein 178; RING-type E3 ubiquitin transferase MARCHF8
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-291
  • 氨基酸序列
    MSMPLHQISAIPSQDAISARVYRSKTKEKEREEQNEKTLGHFMSHSSNISKAGSPPSASA PAPVSSFSRTSITPSSQDICRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDT RCCELCKYEFIMETKLKPLRKWEKLQMTSSERRKIMCSVTFHVIAITCVVWSLYVLIDRT AEEIKQGQATGILEWPFWTKLVVVAIGFTGGLLFMYVQCKVYVQLWKRLKAYNRVIYVQN CPETSKKNIFEKSPLTEPNFENKHGYGICHSDTNSSCCTEPEDTGAEIIHV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.
  • 基因功能参考文献：
    1. Knockdown of MARCH8 inactivated the PI3K and ss-catenin stat3 signaling pathways by changing protein expression levels or the phosphorylation of related proteins and inhibited migration and invasion of MKN-45 and AGS cells. PMID: 30138931
    2. Two additional regulators of BST2 constitutive ubiquitylation and sorting to the lysosomes: the E3 ubiquitin ligases NEDD4 and MARCH8, are reported. PMID: 28320822
    3. Salmonella typhimurium SteD caused MARCH8-dependent ubiquitination and depletion of surface-localized mature MHC class II antigens. PMID: 27832589
    4. YopJ mediates Ser- and Lys-acetylation and affects auto-ubiquitination of ubiquitin ligase MARCH8 in human cells. PMID: 28103160
    5. Kaposi's sarcoma-associated herpesvirus RTA not only downregulated HLA-DRalpha at the protein level through direct binding and degradation through the proteasome pathway but also indirectly downregulated the protein level of HLA-DRalpha by enhancing the expression of MARCH8, a member of the membrane-associated RING-CH (MARCH) proteins. PMID: 27356905
    6. MARCH8 is highly expressed in terminally differentiated myeloid cells, and that it is a potent antiviral protein that targets viral envelope glycoproteins and reduces their incorporation into virions. PMID: 26523972
    7. MARCH 8 mediates the ubiquitination and lysosomal degradation of the transferrin receptor. PMID: 23606747
    8. MARCH8 protein localizes in lysosomes in HeLa cells PMID: 21752829
    9. endogenous MARCH-8 regulates the steady-state cell surface expression of TRAIL-R1. PMID: 23300075
    10. MARCH8-mediated polyubiquitination and degradation of IL1RAP is an important mechanism for negative regulation of IL-1beta-induced signaling pathways. PMID: 22904187
    11. MARCH8 expression led to direct ubiquitination of CD98 and routing of CD98 to late endosomes/lysosomes PMID: 21757542
    12. Membrane-Associated RING-CH proteins MARCH VIII and MARCH IV associate with Bap31 and target CD81 and CD44 to lysosomes PMID: 21151997
    13. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
    14. c-MIR induced specific down-regulation of B7-2 surface expression through ubiquitination, rapid endocytosis, and lysosomal degradation PMID: 12582153
    15. Of the classifier's 11 informative genes, expression of MIR and WDR40 showed statistically significant increases for both Grade 1B and Grade >or=3A rejection. PMID: 18096478
    16. Observational study of gene-disease association. (HuGE Navigator) PMID: 16385451

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  • 亚细胞定位：
    Cytoplasmic vesicle membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein.
  • 组织特异性：
    Broadly expressed. Present in immature dendritic cells (at protein level).
  • 数据库链接：

    HGNC: 23356

    OMIM: 613335

    KEGG: hsa:220972

    STRING: 9606.ENSP00000317087

    UniGene: Hs.499489