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  4. Recombinant Human Glycophorin-B (GYPB)

Recombinant Human Glycophorin-B (GYPB)

  • 货号：
    CSB-CF010075HU
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    GYPB; GPB; Glycophorin-B; PAS-3; SS-active sialoglycoprotein; Sialoglycoprotein delta; CD antigen CD235b
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    10.5 kDa
  • 表达区域：
    20-91aa
  • 氨基酸序列
    LSTTEVAMHTSTSSSVTKSYISSQTNGETGQLVHRFTVPAPVVIILIILCVMAGIIGTILLISYSIRRLIKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    18-23 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is a minor sialoglycoprotein in erythrocyte membranes.
  • 基因功能参考文献：
    1. through bioinformatic analysis, identified extensive variation in GYPB transcript levels in individuals from Benin, suggesting selection from malaria pressure; collective data suggest that the GPA and GPB receptors are of greater importance than the GPC receptor, supporting a hierarchy of erythrocyte receptor usage in P. falciparum PMID: 28760933
    2. A novel GYPB mutation (c.270+5G>A) accounting for the S-s-U+(var) phenotype was identified. PMID: 24738877
    3. Substitution of GPB with Gp.Mur significantly reduced the expression of Rh antigen and RhAG on the Mi.III(+/+) erythrocyte membrane PMID: 21883272
    4. an increased susceptibility to infection by this parasite is associated with the glycophorin B S+ variant in Brazilian Amazons PMID: 21283638
    5. Indochina I strain of P. falciparum is not dependent on glycophorin B to invade erythrocytse through a trypsin-resistant pathway as are the strains 3D7, HB3, and Dd2. PMID: 14638759
    6. The S-s-U+var phenotype arises from changes in or around GYPB exon 5. PMID: 14641872
    7. Alternative to conventional tube technique for mass screening for MNS hybrids, especially when specific antisera are not available. PMID: 17561857
    8. Immunoblotting showed the presence of monomer and dimer forms of a GP(A-B) hybrid and an absence of GPA and GPB. Sequencing of DNA and PCR-RFLP using the restriction enzyme RsaI confirmed the presence of a hybrid GYP(AB). PMID: 18284304
    9. The erythrocyte-binding domain, region 2 of EBL-1, bound glycophorin B(+) but PMID: 19279206
    10. This study reports for the first time the molecular mechanisms responsible for the S-s- phenotype in a population of African Brazilians and provides new information about the frequency and molecular bases of the GYPB*S silent gene in this population. PMID: 19856717

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Glycophorin-A family
  • 数据库链接：

    HGNC: 4703

    OMIM: 111740

    KEGG: hsa:2994

    STRING: 9606.ENSP00000427690

    UniGene: Hs.654368