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  4. Recombinant Human Hyaluronan synthase 1 (HAS1)

Recombinant Human Hyaluronan synthase 1 (HAS1)

  • 货号：
    CSB-CF010139HU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    HAS1
  • Uniprot No.：
  • 别名：
    HAS1; HAS; Hyaluronan synthase 1; Hyaluronate synthase 1; Hyaluronic acid synthase 1; HA synthase 1; HuHAS1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-578
  • 氨基酸序列
    MRQQDAPKPTPAACRCSGLARRVLTIAFALLILGLMTWAYAAGVPLASDRYGLLAFGLYG AFLSAHLVAQSLFAYLEHRRVAAAARGPLDAATARSVALTISAYQEDPAYLRQCLASARA LLYPRARLRVLMVVDGNRAEDLYMVDMFREVFADEDPATYVWDGNYHQPWEPAAAGAVGA GAYREVEAEDPGRLAVEALVRTRRCVCVAQRWGGKREVMYTAFKALGDSVDYVQVCDSDT RLDPMALLELVRVLDEDPRVGAVGGDVRILNPLDSWVSFLSSLRYWVAFNVERACQSYFH CVSCISGPLGLYRNNLLQQFLEAWYNQKFLGTHCTFGDDRHLTNRMLSMGYATKYTSRSR CYSETPSSFLRWLSQQTRWSKSYFREWLYNALWWHRHHAWMTYEAVVSGLFPFFVAATVL RLFYAGRPWALLWVLLCVQGVALAKAAFAAWLRGCLRMVLLSLYAPLYMCGLLPAKFLAL VTMNQSGWGTSGRRKLAANYVPLLPLALWALLLLGGLVRSVAHEARADWSGPSRAAEAYH LAAGAGAYVGYWVAMLTLYWVGVRRLCRRRTGGYRVQV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate.
  • 基因功能参考文献：
    1. hyaluronic acid synthase-1 promotes malignant transformation via epithelial-to-mesenchymal transition, micronucleation and centrosome abnormalities PMID: 29137675
    2. our study indicated a HAS1-miR214-SOX-4 pathway in regulating the growth and metastasis of Esophageal squamous cell carcinoma (ESCC) , providing a promising target for ESCC therapy PMID: 28656277
    3. Reduced expression of HAS1 and HAS2 is associated with melanoma progression and suggests that HAS1 and HAS2 have a prognostic significance in cutaneous melanoma. PMID: 27184066
    4. The minor allele genotypes of HAS1 SNPs are significantly more frequent in MM, WM, CLL and in affected members of a monoclonal gammopathy-prone family than they are in breast cancer, sporadic MGUS or healthy donors PMID: 24950197
    5. transcriptional regulation of the HAS and HAS2-antisense RNA 1 genes, was analyzed. PMID: 25325984
    6. HAS1 is the main enzyme responsible for hyaluronan production by normal keratinocytes. PMID: 24658508
    7. The HAS1-dependent coat is induced by inflammatory agents and glycemic stress, mediated by altered presentation of either CD44 or hyaluronan, and can offer a rapid cellular response to injury and inflammation. PMID: 24099991
    8. Among the genes affected by FAK or HAS3 inhibition were genes, playing role in apoptosis, cell cycle regulation, adhesion, transcription, heatshock and WNT pathways. PMID: 22934709
    9. Inverse expression of hyaluronidase 2 and hyaluronan synthases 1-3 is associated with reduced hyaluronan content in malignant cutaneous melanoma. PMID: 23560496
    10. aberrant intronic HAS1 splicing in multiple myeloma patients may rely on intronic HAS1 deletions and mutations that are frequent in MM patients but absent from healthy donors PMID: 23301075
    11. TGF-beta1 up-regulation of HAS1 transcription was mediated via Smad3 but not Smad2, while HAS1 induction by IL-1beta was Sp3, not Sp1, dependent. PMID: 23123404
    12. Hyaluronan synthase 1 (HAS1) requires higher cellular UDP-GlcNAc concentration than HAS2 and HAS3 PMID: 23303191
    13. HA chains synthesized by HAS1 and HAS2 contribute to outflow resistance, while hyaluronan produced by HAS3 does not appear to play a significant role. PMID: 22695958
    14. analysis of changes in cervical glycosaminoglycan composition during normal pregnancy and preterm birth: Has1 is expressed in preterm birth, while Has2 is induced at term PMID: 22529214
    15. this study provides strong evidence that HAS1-driven Hyaluonan-synthesis is a target of estradiol in human vascular smooth muscle cells PMID: 21901291
    16. HYAL-1 and HAS1 expression predicted bladder cancer metastasis, and HYAL-1 expression also predicted disease-specific survival. PMID: 20960509
    17. Hyaluronan synthases (HAS1-3) and hyaluronidases (HYAL1-2) in the accumulation of hyaluronan in endometrioid endometrial carcinoma PMID: 20875124
    18. Data show that a significant in expression levels of HA synthases (HASs) and hyaluronidases (Hyals) was observed in vitro on stimulation of epithelial ovarian carcinoma cells by gonadotropins. PMID: 20072653
    19. hyaluronan synthase expression in prostate adenocarcinoma cells PMID: 11790779
    20. characterization of expression and hyaluronan synthesis in bone marrow mesenchymal progenitor cells: predominant expression of mRNA and up-regulated hyaluronan synthesis in bone marrow cells derived from multiple myeloma patients PMID: 12239172
    21. Expression of HAS1 in Waldenstrom's macroglobulinemia. Variants of HAS1 synthesize the intracellular HA ligand for RHAMM and may contribute to genetic instability in Waldenstrom's macroglobulinemia. PMID: 12720129
    22. Expression of each hyaluronan synthase isoform is regulated differently by growth factors and cytokines in vascular endothelial cells. Expressed in proliferative membranes from proliferative vitreoretinal diseases. May affect progression of diseases. PMID: 14636845
    23. hyaluronan synthase 1 variants have a role in progression of multiple myeloma PMID: 15731173
    24. Increased HAS1 expression and an increase in serum hyaluronan in endometrial cancer may be associated with disease progression through myometrial invasion and lymph-vascular space involvement PMID: 15870928
    25. IkappaB kinase and IkappaBalpha reveal NF-kappaB-dependent as well as NF-kappaB-independent pathways of HAS1 activation PMID: 16258173
    26. mRNA for HAS1 is undetectable but HAS3 mRNA can be readily detected in freshly isolated CD133+ as well as in CD133- umbilical cord blood (UCB) cells. mRNA for HAS2 can only be detected in CD133+ progenitor cells. PMID: 16564133
    27. Evidence is provided that in type-B synoviocytes (TBS) HAS1 is a gene that depends on the transcription factor nuclear factor kappa B (NF-kappaB) for its activation. PMID: 16723203
    28. The aim of this study was to evaluate how TNF-alpha, IFN-gamma,IL-1beta, and exposition to oxidative stress may modulate HAS activities in normal human skin fibroblasts. PMID: 16786194
    29. Interleukin-1beta stimulation resulted in induction of HAS1 and HAS2 transcription but did not induce phenotypic differentiation or induce hyaluronan coat assembly PMID: 17611197
    30. HAS1 captured in this pull-down approach is readily immunodetected by Western blot analysis using appropriate antibodies. PMID: 17904513
    31. Treatment of fibroblasts with growth factors up-regulated HAS gene expression and increased HAS enzymes and HA production. PMID: 17922656
    32. HAS1 regulates bladder cancer growth and progression by modulating hyaluronic acid synthesis and hyaluronic acid receptor levels. PMID: 18199543
    33. HAS1 is activated by Epstein-Barr virus and synthetic double- and single-stranded viral RNA analogs PMID: 18400745
    34. genetic contributions toward aberrant splicing of the HAS1 gene in multiple myeloma and Waldenstrom macroglobulinemia were characterized; 3616 bp in HAS1 exons and introns involved in aberrant splicing, from 17 patients were sequenced PMID: 18815290
    35. HAS expression in human articular chondrocytes during the de- and re-differentiation processes. PMID: 19148550
    36. By intensive sequencing of the hyaluronan synthase 1 (HAS1) gene in malignant and normal cells from patients with WM, we have identified both types of mutation in HAS1 exons and introns. PMID: 19362966
    37. HAS1, HAS2 and HAS3 expression is not consistently elevated in ovarian carcinomas. PMID: 19435493
    38. HAS1 (Hyaluronan Synthase 1) splice variants multimerize with and modulate normally spliced HAS1 protein PMID: 19451652
    39. Transfection with siHAS2 and siHAS1 showed that mainly HAS1 synthesized high molecular weight HA regulates HT1080 cell motility. PMID: 19577615
    40. HAS activity can be modulated by post-translational modification, such as phosphorylation and N-glycosylation PMID: 19737932

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    NodC/HAS family
  • 组织特异性：
    Widely expressed. Highly expressed in ovary followed by spleen, thymus, prostate, testes and large intestine. Weakly expressed in small intestine.
  • 数据库链接：

    HGNC: 4818

    OMIM: 601463

    KEGG: hsa:3036

    STRING: 9606.ENSP00000222115

    UniGene: Hs.57697