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  4. Recombinant Human Leukotriene C4 synthase (LTC4S)

Recombinant Human Leukotriene C4 synthase (LTC4S)

  • 中文名称：
    人LTC4S重组蛋白
  • 货号：
    CSB-CF013228HU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    LTC4S
  • Uniprot No.：
  • 别名：
    LTC4S; Leukotriene C4 synthase; LTC4 synthase; Glutathione S-transferase LTC4; Leukotriene-C(4 synthase; Leukotriene-C4 synthase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-150
  • 氨基酸序列
    MKDEVALLAAVTLLGVLLQAYFSLQVISARRAFRVSPPLTTGPPEFERVYRAQVNCSEYF PLFLATLWVAGIFFHEGAAALCGLVYLFARLRYFQGYARSAQLRLAPLYASARALWLLVA LAALGLLAHFLPAALRAALLGRLRTLLPWA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the conjugation of leukotriene A4 with reduced glutathione (GSH) to form leukotriene C4 with high specificity. Can also catalyzes the transfer of a glutathionyl group from glutathione (GSH) to 13(S),14(S)-epoxy-docosahexaenoic acid to form maresin conjugate in tissue regeneration 1 (MCTR1), a bioactive lipid mediator that possess potent anti-inflammatory and proresolving actions.
  • 基因功能参考文献：
    1. mutational and kinetic data, together with molecular simulations, suggest that phosphorylation of Ser(36) inhibits the catalytic function of LTC4S by interference with the catalytic machinery. PMID: 27365393
    2. Biochemical and structural characterization of LTC4S mutants along with crystal structures of the wild type and mutated enzymes in complex with three product analogs, provide new insights to binding of substrates and product. PMID: 24366866
    3. The results of our study failed to confirm whether the selected variants in LTC4S gene within the LT metabolism pathway contribute to platelet reactivity in a diabetic population treated with ASA. PMID: 23828562
    4. Elevated levels of leukotriene C4 synthase mRNA distinguish a subpopulation of eosinophilic oesophagitis patients. PMID: 23889244
    5. genetic association studies in population of Han Chinese in Eastern China: Data suggest that an SNP in LTC4S (rs730012) is associated with risk of ischemic stroke; carriers of C allele of rs730012 in LTC4S are most susceptible to ischemic stroke. PMID: 23079278
    6. meta-analysis suggested that the -444A/C polymorphism in the LTC4S gene would be a risk factor for asthma in Caucasians and aspirin-tolerant populations PMID: 22884858
    7. A significant association has been found between the -1072G/A (rs3776944) SNP in the LTC4S gene and atopic asthma in the family-based analysis. PMID: 22722751
    8. The SNPs of ALOX(5)AP and LTC(4)S are associated with asthma. PMID: 21729626
    9. investigation of catalytic mechanism of LTC4S: Data suggest that glutathione thiolate anion formation in LTC4S is not rate-limiting for overall reaction of leukotriene C4 production. Thiolate anion is stabilized by Arg104 at all three active sites. PMID: 22217203
    10. Polymorphisms of the PTGDR and LTC4S influence responsiveness to leukotriene receptor antagonists in Korean children with asthma. PMID: 21307858
    11. The catalytic architecture of leukotriene C4 synthase with two arginine residues. PMID: 21454538
    12. The increased expression of LTC(4)S, together with the predominant formation of cysteinyl-leukotrienes and effects on MMPs production, suggests a mechanism by which LTs may promote matrix degradation in the AAA wall PMID: 21078989
    13. Arginine 104 is a key catalytic residue in leukotriene C4 synthase. PMID: 20980252
    14. In a Southwest Chinese Han population LTC(4)S A(-444)C polymorphism might be a determinant factor in the clinical response of asthma to leukotriene receptor antagonists. PMID: 19080532
    15. no association between gene polymorphism and bronchial asthma in a Spanish population PMID: 20128419
    16. Distinct roles of receptor phosphorylation, G protein usage, and mitogen-activated protein kinase activation on platelet activating factor-induced leukotriene C(4) generation PMID: 11934880
    17. no relationship between the polymorphism and LTC4S activity in eosinophils, although LTC4S activities were significantly higher in patients with aspirin induced asthma than in patients with aspirin tolerant asthma PMID: 12063521
    18. A(-444)C polymorphism of this gene and clinical response to pranlukast in Japanses patients with moderate asthma PMID: 12360108
    19. C-to-A promoter polymorphism was associated with the increased presence of chronic hyperplastic eosinophilic sinusitis and the expression of cysteinyl leukotrienes PMID: 12589355
    20. expression of LTC(4)S during normal and leukemic myelopoiesis and correlation with the activity of the disease-specific tyrosine kinase p210 BCR-ABL in CML myeloid cells. PMID: 12591277
    21. Gene expression in mononuclear phagocytes is regulated by SP1 and SP3. PMID: 12664565
    22. Data show that mucosal mast cells, and not eosinophils, were the dominating leukotriene C4 synthase-containing cells in both untreated and treated aspirin-tolerant asthma. PMID: 12816731
    23. We further conclude that the A(-444)C polymorphism in the LTC(4) synthase gene probably contributes to asthma interpatient variability in montelukast-evoked changes in FE(NO)* and warrants further study. PMID: 14520724
    24. independent of transcriptional activity, the C(-444) allele in the LTC(4) synthase gene is weakly associated with the asthma phenotype, but it is not related to disease severity or aspirin intolerance PMID: 15131571
    25. calculated a projection map of recombinant human LTC(4) synthase at a resolution of 4.5 A by electron crystallography PMID: 15530365
    26. leukotriene C4 synthase gene promoter polymorphism is associated with asthma and/or atopy PMID: 16024972
    27. Glu 4 Lys amino acid substitution in the LTC4S might be associated with allergic diseases. PMID: 16211251
    28. The C allele of the leukotriene C4 synthase (A-444C) polymorphism is associated with asthma phenotype or severity. PMID: 16675353
    29. LTC4S plays a key role in the process of inflammation as the rate limiting enzyme in the conversion of arachidonic acid to cysteinyl-leukotrienes, important mediators of inflammatory responses. PMID: 17110605
    30. The combination of 927T CYSLTR1 and -444A LTC4S was less common in male patients with asthma than in controls and the combination of 927C CYSLTR1 and -444A LTC4S was slightly more frequent in patients with asthma PMID: 17153879
    31. The results of a case-control study aimed at investigating the association of MGST1 gene locus polymorphisms with colorectal cancer risk among Han Chinese are presented. PMID: 17483957
    32. crystal structure of the human LTC4 synthase in its apo and GSH-complexed forms to 2.00 and 2.15 A resolution, respectively PMID: 17632546
    33. atomic structure of human LTC4S in a complex with glutathione at 3.3 A resolution by X-ray crystallography PMID: 17632548
    34. Leukotriene C(4) synthase -1072 AA genotype predict increased risk, whereas -444 CC genotype predict decreased risk of ischemic cerebrovascular disease. PMID: 18276912
    35. Genetic variation in leukotriene pathway members and their receptors confer an increased risk of ischemic stroke in 2 independent populations PMID: 18323512
    36. The combined study of polymorphisms in genes of the leukotriene pathway could explain the differences observed in the studies reported on polymorphism -444A < C LTC4S individually analysed. PMID: 19080797
    37. LTC(4)S interacts in vitro with both FLAP and 5-LO and that these interactions involve distinct parts of LTC(4)S. PMID: 19233132
    38. Leukotriene C4 synthase promoter genotypes influence risk of transient ischemic attack and ischemic stroke, but not risk of ischemic heart disease/coronary atherosclerosis, asthma, or chronic obstructive pulmonary disease. PMID: 19280718
    39. Observational study of gene-gene interaction and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 17924829
    40. Clinical trial and meta-analysis of gene-environment interaction and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 12968987

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  • 相关疾病：
    LTC4 synthase deficiency is associated with a neurometabolic developmental disorder characterized by muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.
  • 亚细胞定位：
    Nucleus outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein.
  • 蛋白家族：
    MAPEG family
  • 组织特异性：
    Detected in lung, platelets and the myelogenous leukemia cell line KG-1 (at protein level). LTC4S activity is present in eosinophils, basophils, mast cells, certain phagocytic mononuclear cells, endothelial cells, vascular smooth muscle cells and platelet
  • 数据库链接：

    HGNC: 6719

    OMIM: 246530

    KEGG: hsa:4056

    STRING: 9606.ENSP00000292596

    UniGene: Hs.706741