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  4. Recombinant Human Phospholemman (FXYD1)

Recombinant Human Phospholemman (FXYD1)

  • 货号：
    CSB-CF009089HU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    FXYD1; PLM; Phospholemman; FXYD domain-containing ion transport regulator 1; Sodium/potassium-transporting ATPase subunit FXYD1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    21-92
  • 氨基酸序列
    ESPKEHDPFTYDYQSLQIGGLVIAGILFILGILIVLSRRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRRR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na(+) out of the cell and K(+) into the cell. Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated. Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1. Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone.
  • 基因功能参考文献：
    1. Study demonstrated that the expression of FXYD1, FXYD3 and FXYD5 is elevated in the lungs of Acute respiratory distress syndrome patients PMID: 26410457
    2. a period of high-intensity training with reduced training volume increases expression and phosphorylation levels of FXYD1, which may affect Na(+)/K(+) pump activity and muscle K(+) homeostasis during intense exercise. PMID: 26791827
    3. Stopped-flow experiments using the dye RH421 show that FXYD1 slows the conformational transition E2(2K)ATP --> E1(3Na)ATP but does not affect 3NaE1P --> E2P3Na. PMID: 26429909
    4. the evolutionary conservation of G-quadruplex forming sequences with the confirmation of G-quadruplex formation in vitro by two FXYD1 homologues PMID: 25051342
    5. Phospholemman is the target of a variety of post-translational modifications and these can dynamically alter the activity of the Na pump. [Review] PMID: 23672825
    6. PLM regulates important ion transporters in the heart and offers a tempting target for development of drugs to treat heart failure. PMID: 23224879
    7. Intracellular trafficking of FXYD1 (phospholemman) and FXYD7 proteins in Xenopus oocytes and mammalian cells. PMID: 22535957
    8. the severity of the spinal cord lesion is an important factor controlling the expression of Na(+)-K(+)-ATPase and its regulatory protein PLM PMID: 22275761
    9. in left ventricular myocardium from patients with heart failure, PLM Ser-68 phosphorylation was approximately 50% lower than in nonfailing controls PMID: 21849407
    10. Exercise induces FXYD1 phosphorylation at multiple sites in human muscle; in mice, contraction-induced changes in FXYD1 phosphorylation are fiber-type specific and dependent on protein kinase Calpha activity. PMID: 21957166
    11. FXYD1 raises the affinity of the human alpha1beta1 isoform of Na,K-ATPase for Na ions PMID: 21449573
    12. Results suggest that the PLM cytoplasmic domain populates NKA-associated and membrane-associated states in dynamic equilibrium and that phosphorylation may alter the position of the equilibrium. PMID: 21130070
    13. phosphorylation of PLM increases its oligomerization into tetramers, decreases its binding to NKA, and alters the structures of both the tetramer and NKA regulatory complex. PMID: 21220422
    14. PLM phosphorylation at either Ser63 or Ser68 is both necessary and sufficient for completely relieving the PLM-induced NKA inhibition. PMID: 20861470
    15. Data suggest that phospholemman plays an important role in fine tuning the gating kinetics of cardiac calcium channels and likely plays an important role in shaping the cardiac action potential and regulating Ca(2+) dynamics in the heart. PMID: 20720179
    16. Phospholemman modulates the gating of cardiac L-type calcium channels PMID: 20371314
    17. Study reveals, in various human tissues, the specific expression of FXYD1, which may associate with Na, K-ATPase in selected cell types and modulate its catalytic properties. PMID: 19879113
    18. molecular cloning, protein expression, sequencing and NMR structure determination PMID: 12535606
    19. show that the helical regions and connecting segments of FXYD1, FXYD3, and FXYD4 determined in micelles by NMR spectroscopy coincide with the positions of intron-exon junctions in the genes PMID: 16288923
    20. PLM interacts with the intracellular loop of NCX1, most likely at residues 218-358 PMID: 16921169
    21. study reports that FXYD1 is elevated in frontal cortex neurons of Rett syndrome patients and Mecp2-null mice; FXYD1 is identified as a MeCP2 target gene whose de-repression may directly contribute to RTT neuronal pathogenesis PMID: 17309881
    22. the structure of FXYD1 suggests a mechanism whereby the phosphorylation of conserved Ser residues, by protein kinases A and C, could induce a conformational change in the cytoplasmic domain, to modulate its interaction with the Na,K-ATPase, alpha subunit PMID: 18000745
    23. reconstituted FXYD1 protects both alpha1beta1 and alpha2beta1 very strongly against thermal inactivation PMID: 18052210
    24. Data show that PLM associates with and modulates both NKA-alpha1 and NKA-alpha2 in a comparable but not identical manner. PMID: 19638348

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  • 亚细胞定位：
    Cell membrane, sarcolemma; Single-pass type I membrane protein. Apical cell membrane; Single-pass type I membrane protein. Membrane, caveola. Cell membrane, sarcolemma, T-tubule.
  • 蛋白家族：
    FXYD family
  • 组织特异性：
    Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, pros
  • 数据库链接：

    HGNC: 4025

    OMIM: 602359

    KEGG: hsa:5348

    STRING: 9606.ENSP00000343314

    UniGene: Hs.442498