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  4. Recombinant Human Sterol regulatory element-binding protein 1 (SREBF1), partial

Recombinant Human Sterol regulatory element-binding protein 1 (SREBF1), partial

Unavailable
  • 货号：
    CSB-CF022657HU
  • 规格：
    ￥5028
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1
  • 种属：
    Homo sapiens(Human)
  • 蛋白长度：
    Partial
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    54.5kDa
  • 表达区域：
    1-490aa
  • 氨基酸序列
    MDEPPFSEAALEQALGEPCDLDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPGSLSPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPTPLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQPLPGALLPQSFPAPAPPQFSSTPVLGYPSPPGGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLHTQVQSVVPQQLLTVTAAPTAAPVTTTVTSQIQQVPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSPLVSGTTVQTGPLPTLVSGGTILATVPLVVDAEKLPINRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSINDKIIELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGNTDVLMEGVKTEVEDTLTPPPSDAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSRGMLDRSRLAL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis.; Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation.; Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C. Able to stimulate both lipogenic and cholesterogenic gene expression. Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver. Required for innate immune response in macrophages by regulating lipid metabolism.; Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A. Primarily controls expression of lipogenic gene. Strongly activates global lipid synthesis in rapidly growing cells.; The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters.; The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters.
  • 基因功能参考文献：
    1. results reveal that nBP1a/PKM2 interaction activates lipid metabolism genes in cancer cells and that Thr-59 phosphorylation of SREBP-1a plays an important role in cancer cell proliferation. PMID: 29514980
    2. GTEE also downregulated the expression of AR and prostate-specific antigen (PSA) in both androgen-responsive and castration-resistant PCa cells. By blocking the SREBP-1/AR axis, GTEE suppressed cell growth and progressive behaviors, as well as activating the caspase-dependent apoptotic pathway in PCa cells PMID: 30301150
    3. SREBP1 trans-activates CYP24A1 expression through SREBP binding elements present in the promoter. PMID: 29653103
    4. Berberine (BBR), an effective suppressor of SREBP1 and lipogenesis regulated through reactive oxygen species (ROS)/AMPK pathway, selectively inhibited the growth of G-R nonsmall cell lung cancer cells and rheumatoid arthritis patiens but not that of normal cells PMID: 28665143
    5. These findings suggest that SREBP-1c serves as a molecular bridge between lipid metabolism and cell cycle control inclear cell renal cell carcinoma tumorigenesis. PMID: 29138263
    6. findings showed that PTEN inhibits HBV replication as well as HBV HCV co-replication. SREBP-1 is involved in HBV HCV replication inhibition by PTEN PMID: 29803738
    7. FTO increased the lipid accumulation in hepatocytes by increasing nuclear translocation of SREBP1c and SREBP1c maturation, thus improving the transcriptional activity of lipid droplet-associated protein CIDEC. PMID: 29486327
    8. common SNPs (rs62064119, rs2297508, rs11868035 and rs13306741) in the SREBP-1c gene were selected and genotyped in 593 Han patients with NAFLD and 593 healthy controls. No significant differences in genotype and allele frequencies of these four SNPs were found between cases and controls, suggesting that the SNPs are not associated with risk of NAFLD in the Chinese Han population. PMID: 27572914
    9. Data suggests that expression of CYP4F2 is down-regulated in liver of mice with non-alcoholic fatty liver disease after high-fat/Western diet and in human hepatocyte cell line exposed to excess palmitic acid, oleic acid, or fructose. Two other genes are down-regulated, PPAR gamma and SREBP-1. (CYP4F2 = cytochrome P450 family 4 subfamily F member 2; PPAR = peroxisome proliferator-activated receptor ) PMID: 28628909
    10. LncARSR promotes hepatic lipogenesis via Akt/SREBP-1c pathway and contributes to the pathogenesis of nonalcoholic steatohepatitis. PMID: 29555473
    11. CpG sites located in SREBF2 gene showed differential methylation in association with lipid traits. The expression of SREBF1 gene was inversely associated with methylation of its corresponding CpGs. Genetic variants in SREBF1 were also associated with lipid profile. SREBF1 expression was directly associated with HDL cholesterol. PMID: 28173150
    12. Epidermal growth factor receptor (EGFR) signaling enhances miR-29 expression in glioblastoma cells via upregulation of Sterol regulatory element binding protein PMID: 27477273
    13. Our finding reveals a crucial roles for SREBP1 in lipid desaturation of ccRCC through regulation of NF-kappaB signaling, which provides not only new insights in regulatory mode of NF-kappaB signaling but also a novel target for potential metabolic therapies. PMID: 29183723
    14. Our results suggest that relatively common genetic variants in stearoyl CoA desaturase and SREBF1 attenuated the positive associations between intake of a traditional diet rich in n-3 polyunsaturated fatty acids and increases in fasting cholesterol and HbA1c levels, as well as the waist-to-hip ratio among Yup'ik participants. PMID: 27467133
    15. changes in distinct lipid ratios may converge on ARF1 to increase SBP-1/SREBP-1 activity. PMID: 27320911
    16. Variants in the TOM1L2/SREBF1 locus exert opposing effects of total-body lean mass (TB-LM) and total-body less head bone mineral density (TBLH-BMD) . PMID: 28743860
    17. Date indicate that sterol regulatory element-binding proteins Srebp1 and Srebp2 are essential for the metabolic reprogramming of NK cells and for the attainment of elevated glycolysis and oxidative phosphorylation. PMID: 28920951
    18. Study identified a novel human specific lncRNA, lncHR1, as a negative regulator of SREBP-1c expression. Overexpression of lncHR1 inhibited expression of SREBP-1c and fatty acid synthase (FAS) and then repressed oleic acid-induced hepatic cell triglyceride (TG) and lipid droplet (LD) accumulation. PMID: 28367099
    19. Glucose adsorption to chitosan membranes increases proliferation of human chondrocytes via mammalian target of rapamycin complex 1 and sterol regulatory element-binding protein-1 signaling. PMID: 28218386
    20. miR-185 negatively regulates the differentiation of 3T3-L1 cells by targeting SREBP-1 PMID: 28701079
    21. The s further demonstrated that the upregulation of sterol regulatory element-binding protein (SREBP)-1c by activation of the Akt and p70S6K pathways is critical for high-glucose-treated Porphyromonas gingivalis-induced NLRP3 expression. PMID: 28083517
    22. Results show that PPARalpha is downregulated and SREBP-1c is upregulated in steatosis L-02 cells. These changes increase lipid synthesis and reduce lipid disposal, which ultimately lead to hepatic steatosis. PMID: 27270405
    23. SREBP-1 and SREBP-2 mRNA expression levels were measured in EAT from 49 patients with CAD (26 with diabetes) and 23 controls without CAD or diabetes.SREBP expression was associated as cardiovascular risk factor for the severity of CAD and the poor lipid control. PMID: 28367087
    24. The involvement of SREBP-1c in FASN promoter histone modification. PMID: 28027934
    25. the mitotic phosphorylation and stabilization of nuclear SREBP1 during cell division provides a link between lipid metabolism and cell proliferation. PMID: 27579997
    26. B7-H3 hijacks SREBP-1/FASN signaling mediating abnormal lipid metabolism in lung cancer PMID: 27939887
    27. The genetic polymorphisms of SREBF1 could play a role in the mechanism for interindividual variation of atypical antipsychotics-induced metabolic syndrome (MetS). SCAP polymorphisms with drug-induced MetS were negative in this study. PMID: 26982812
    28. NS5ATP6 regulates the intracellular triglyceride level via FGF21, and independently of SIRT1 and SREBP1. PMID: 27179781
    29. observations suggest that MALAT1 promotes hepatic steatosis and insulin resistance by increasing nuclear SREBP-1c protein stability. PMID: 26935028
    30. MiR-132 inhibited SIRT1 and SREBP-1c expression and downregulated their targeted genes, including HMGCR and FASN. PMID: 26898440
    31. Report demonstrated that overexpression of SULT2B1b-mediated angiogenic signaling was associated with tumor angiogenesis and poor clinical features of human gastric cancer. PMID: 26937945
    32. Data show that mutant p53 protein activates the sterol regulatory element-binding proteins SREBP-1 and SREBP-2-mediated signaling pathways in prostate cancer (PCa) cells. PMID: 26512780
    33. miR-33b is highly induced upon differentiation of human preadipocytes, along with SREBP-1 and miR-33b is an important regulator of adipogenesis PMID: 26830228
    34. Akt1 and Akt2 activated both SREBP-1 and SREBP-2, whereas Akt3 upregulated SREBP-1 to enhance hepatitis C virus translation. PMID: 26855332
    35. PRMT5-induced methylation prevented phosphorylation of SREBP1a on S430 by GSK3beta PMID: 26759235
    36. mTORC2 positively regulates mSREBP1 stability and lipogenesis. Findings reveal a novel biological function of mTORC2 in the regulation of lipogenesis PMID: 25893295
    37. mTORC1/SREBP pathway is a major mechanism through which common oncogenic signaling events induce de novo lipid synthesis to promote aberrant growth and proliferation of cancer cells PMID: 26028026
    38. hnRNP A1 is implicated in the free fatty acid-induced expression of SREBP-1a and of its target genes as well as in the lipid accumulation in hepatocytes. PMID: 26869449
    39. TG levels are regulated by HCBP6-sterol regulatory element binding protein 1c (SREBP1c)-mediated fatty acid synthase (FASN) expression. PMID: 25855506
    40. Aberrant activation of SREBP1c suppresses primary ciliogenesis by PLA2G3-mediated distortion of vesicular trafficking and suggest that PLA2G3 is a novel potential target to normalize ciliogenesis in SREBP1c-overexpressing cells, including cancer cells. PMID: 25904332
    41. PD-L1 induces epithelial-to-mesenchymal transition via activating SREBP-1c in renal cell carcinoma. PMID: 26141060
    42. performed a detailed promoter/enhancer analysis of ELOVL5 gene, and identified two new SREBP binding sites, one in the 10 kb upstream region and one in the exon 1 PMID: 26321664
    43. Data indicate that glucose-mediated glycosylation promotes SREBP cleavage-activating protein (SCAP) trafficking to the Golgi Leading to sterol regulatory element binding protein 1 (SREBP-1) activation. PMID: 26555173
    44. Single nucleotide polymorphism (rs2297508) of SREBF-1 may serve as a genetic predisposition factor for the development of endometrial cancer. PMID: 24614076
    45. Report PCR techniques for genotyping SREBF1 rs8066560 variant in Iranian children/adolescents with metabolic syndrome. PMID: 26771965
    46. Data show that the cleavage site of the lipid-signaling protein sterol regulatory element binding transcription factor 1 (SREBP-1) intermediate bears rigid alpha-helical topology. PMID: 26392539
    47. We concluded that 54(G/C) polymorphism of SREBF-1 gene is associated with polycystic ovary syndrome (PCOS) and suggest that SREBF-1 gene may play a role in genetic predisposition to PCOS. PMID: 25801724
    48. The results from this study demonstrate that metformin ameliorates PA-induced insulin resistance through the activation of AMPK and the suppression of SREBP-1c in skeletal muscle cells. PMID: 25891779
    49. Associations between triglyceride levels and SREBF1 and ABCG1 were also found in adipose tissue of the Multiple Tissue Human Expression Resource cohort PMID: 25583993
    50. gene expression analysis revealed that SREBP defines a gene signature that is associated with poor survival in glioblastoma PMID: 25619842

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  • 亚细胞定位：
    [Sterol regulatory element-binding protein 1]: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein.; [Processed sterol regulatory element-binding protein 1]: Nucleus.; [Isoform SREBP-1aDelta]: Nucleus.; [Isoform SREBP-1cDelta]: Nucleus.
  • 蛋白家族：
    SREBP family
  • 组织特异性：
    Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression.; [Isoform SREBP-1A]: Predominates in hepatoma cell lines. Also expressed in kidney, brain, white fat, and muscle.;
  • 数据库链接：

    HGNC: 11289

    OMIM: 184756

    KEGG: hsa:6720

    STRING: 9606.ENSP00000348069

    UniGene: Hs.592123