Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 跨膜蛋白
  4. Recombinant Medicago truncatula Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3 (DMI3)

Recombinant Medicago truncatula Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3 (DMI3)

  • 中文名称：
    Recombinant Medicago truncatula Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3(DMI3)
  • 货号：
    CSB-CF744189MQP
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    DMI3
  • Uniprot No.：
  • 别名：
    CCAMK; DMI3; MTR_8g043970; Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3; CCaMK DMI3; MtCCaMK; Protein DOES NOT MAKE INFECTIONS 3
  • 种属：
    Medicago truncatula (Barrel medic) (Medicago tribuloides)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-523
  • 氨基酸序列
    MGYGTRKLSDEYEVSEILGRGGFSVVRKGTKKSSIEEEKSQSQVAIKTLRRLGASNNPSG LPRKKDIGEKSTIGFPTMRQVSVSDTLLTNEILVMRRIVENVSPHPNVIDLYDVYEDTNG VHLVLELCSGGELFDRIVAQDKYSETEAATVVHQIASGLEAVHRANIVHRDLKPENCLFL DVRKDSPLKIMDFGLSSVEEFTDPVVGLFGSIDYVSPEALSQGKITTKSDMWSLGVILYI LLSGYPPFIAQNNRQKQQMIMNGNFSFYEKTWKGISQPAKNLISSLLTVDPSKRPSALEL LSDPWVKGEKAKDVQMDPEIVSRLQSFNARRKLRAAAIASVWSSTIFLRTKKLKSLVGSY DLKEEEIENLRMHFKKICADRDNATLSEFEEVLKAMNMLSLIPFASRIFDLFDNNRDGTV DMREILCGFSSLKNSKGEDALRLCFQMYDTDRSGCISKEEVASMLRALPYDCLPTDITEP GKLDEIFDLMDANNDGKVTFDEFKAAMQRDSSLQDVVLSSIRP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    During nodulation, plays a central role in bacterial infection and contributes to nodule organogenesis. Protein kinase that recognizes the calcium spiking induced by Nod factors and translates this signal to components controlling nodulation and mycorrhizal infection responses. May phosphorylate the NSP1 protein. Required in epidermal and cortical cells to promote infection thread (IT) formation in root hairs.
  • 基因功能参考文献：
    1. Phosphorylation at S344 completely turns off the root nodule symbiosis-supporting function of CCaMK, even in the T271A gain-of-function background. PMID: 23869591
    2. The visinin-like domain binds three calcium ions, leading to a conformational change involving the exposure of hydrophobic surfaces and a change in tertiary but not net secondary or quaternary structure. PMID: 22889004
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 组织特异性：
    Highly expressed in roots. Expressed in root hairs and nodules. Expressed at low levels in flowers. Not detected in leaves or stems.
  • 数据库链接：

    KEGG: mtr:MTR_8g043970

    UniGene: Mtr.6758