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  4. Recombinant Mouse 5-hydroxytryptamine receptor 3A (Htr3a)

Recombinant Mouse 5-hydroxytryptamine receptor 3A (Htr3a)

  • 中文名称：
    小鼠Htr3a重组蛋白
  • 货号：
    CSB-CF010890MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Htr3a
  • Uniprot No.：
  • 别名：
    Htr3a; 5ht3; Htr3; 5-hydroxytryptamine receptor 3A; 5-HT3-A; 5-HT3A; 5-hydroxytryptamine receptor 3; 5-HT-3; 5-HT3R; Serotonin receptor 3A; Serotonin-gated ion channel receptor
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-487
  • 氨基酸序列
    SRRRATQEDTTQPALLRLSDHLLANYKKGVRPVRDWRKPTTVSIDVIMYAILNVDEKNQV LTTYIWYRQYWTDEFLQWTPEDFDNVTKLSIPTDSIWVPDILINEFVDVGKSPNIPYVYV HHRGEVQNYKPLQLVTACSLDIYNFPFDVQNCSLTFTSWLHTIQDINITLWRSPEEVRSD KSIFINQGEWELLEVFPQFKEFSIDISNSYAEMKFYVIIRRRPLFYAVSLLLPSIFLMVV DIVGFCLPPDSGERVSFKITLLLGYSVFLIIVSDTLPATIGTPLIGVYFVVCMALLVISL AETIFIVRLVHKQDLQRPVPDWLRHLVLDRIAWILCLGEQPMAHRPPATFQANKTDDCSG SDLLPAMGNHCSHVGGPQDLEKTPRGRGSPLPPPREASLAVRGLLQELSSIRHFLEKRDE MREVARDWLRVGYVLDRLLFRIYLLAVLAYSITLVTLWSIWHYS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • 基因功能参考文献：
    1. A triad of residues aligning to Thr-152, Glu-209, and Lys-211 in Htr3, appear to be involved in side-chain interactions near binding sites in Htr3a (subunit alpha) and muscle-type Chrna1. Data suggest that mutating Htr3a triad to that of Chrna1 increases binding affinity of nicotine to Htr3a. (Htr3 = 5-hydroxytryptamine/serotonin receptor; Chrna1 = cholinergic receptor nicotinic alpha polypeptide 1) PMID: 29298898
    2. Results show that 5-HT3AR was expressed in GABAergic interneurons containing somatostatin or calretinin, and most strongly in the olfactory bulb, cerebral cortex, hippocampus, and amygdala. The pons, medulla, and spinal cord showed partial 5-HT3AR expression. Meanwhile, slight expression of 5-HT3AR was seen in the thalamus, hypothalamus, and midbrain; but no expression in the cerebellum. PMID: 28276429
    3. Knock down serotonin receptors 5-HTR3 and 5-HTR4 in neonatal cardiomyocytes resulted in significant increase of cell damage in response to hypoxia, and also led to alternation in heart beating. PMID: 27874067
    4. analysis of the structure from the full-length 5-HT3AR channel in the apo-state determined by single-particle cryo-electron microscopy PMID: 29410406
    5. Transmission of sour taste information involves communication between taste cells and 5-HT3A-expressing afferent nerve fibers. PMID: 28316078
    6. the recently available murine 5-HT3 receptor by identifying sites of strong interaction with particular functional groups at both the orthogonal (serotonin) site and a proposed allosteric binding site situated at the interface between the transmembrane region and the extracellular domain, was characterized. PMID: 27816008
    7. We conclude that cholera toxin inhibits colonic migrating motor complexes via release of mucosal 5-HT, which activates an inhibitory pathway involving 5-HT3 receptors PMID: 26990461
    8. It was concluded that epithelial 5-HT3 receptor may function as a mediator of gut microbiota-driven change in intestinal secretion. PMID: 28408644
    9. 5HT3A receptor deletion in neuroblasts impaired speed and directionality of migration and abolished calcium spikes. PMID: 28472655
    10. The antidepressant bupropion is a negative allosteric modulator of serotonin type 3A receptors. Results demonstrate allosteric blockade by bupropion of the 5-HT3AR. PMID: 27671323
    11. Studies with 16 arylguanidines found that their functional activity spanned a broad spectrum from superagonist to full agonist, partial agonist, and antagonist at 5-HT3 receptors; results confirm the utility of phenylguanidine as an extremely versatile scaffold in the design of 5-HT3 ligands with a "tunable" level of agonist or antagonist activity PMID: 27533595
    12. 5-HT3A-ICD is not only required but also sufficient for interaction with RIC-3 PMID: 27045630
    13. The data of this study demonstrated that the 5-HT3 receptor is the critical target of 5-HT action in exercise-induced hippocampal neurogenesis and antidepressant effects, but not in learning enhancement. PMID: 25403840
    14. The deletion of Htr3a was related to fatal arrhythmias and sudden cardiac death during pregnancy, and its activation reversed the QT prolongation. PMID: 25986676
    15. The results of this study suggested that 5-HT released from type III cells activates gustatory nerve fibers via 5-HT3 receptors, accounting for a significant proportion of the neural taste response. PMID: 26631478
    16. Htr3a is required for controlling the migration and laminar positioning of reelin-expressing interneurons in the neocortex. PMID: 25409778
    17. Deletion of the 5-HT3AR gene in transgenic mice abolished N-methyl-d-aspartate (NMDA) receptor (NMDAR)-dependent long-term depression (LTD) induced by low-frequency stimulation (LFS) in hippocampal CA1 synapses in slices. PMID: 25130560
    18. 5HT2AR and 5HT3AR were detected by RT-PCR techniques. PMID: 24969868
    19. Results indicate that vestibular ganglion cells express functional 5-HT3 receptor channels (with 5-HT3a and 5-HT3b subunits) and might play a modulatory role in the peripheral vestibular nervous system PMID: 24530269
    20. 5-HT system in the small intestine may play a critical role in regulating circadian rhythms through 5-HT3/5-HT4-receptor activation. PMID: 24492464
    21. X-ray structure of a mammalian Cys-loop receptor, the mouse serotonin 5-HT3 receptor, at 3.5 A resolution PMID: 25119048
    22. The data of this study suggested that the 5-HT3A receptor could be a key molecule regulating fear memory processes and a potential therapeutic target for fear disorders. PMID: 24344177
    23. Serotonin, acting in a paracrine/autocrine manner through Htr3, lowers the beta cell threshold for glucose and plays an essential role in the increased glucose-stimulated insulin secretion of pregnancy. PMID: 24218571
    24. 5-HT3 and NK1 immunopositive macrophages and mucosal mast cells release 5-HT and substance P, which in turn activate their corresponding receptors on mucosal cells in autocrine and paracrine manners. PMID: 23695873
    25. Alcohol consumption induces Htr3a promoter DNA methylation changes in blood and brain. PMID: 22834954
    26. Serotonin 5-HT3 receptors control morphological maturation of Purkinje cells via reelin PMID: 23318873
    27. In a mutant cycle analysis, a residue Asp124 of loop A in the 5-HT3A receptor forms two functionally important hydrogen bonds to the backbone of loop B, an interaction critical for receptor function. PMID: 23077719
    28. This study demonistrated that 5-hydroxytryptamine 3A receptor-expressing cells within the adult SVZ-RMS-OB system. PMID: 23041763
    29. This study demonistraed that knockout mice lacking the 5-HT(3A) receptor showed exuberant apical dendrites of these cortical pyramidal neurons. PMID: 22696545
    30. Activation of central 5-HT(3) receptors is more effective in hypothermia induction due to marked decrease in thermogenesis and increase in heat loss. It switched metabolism to prevalent use of lipids as the main energetic substrate. PMID: 21964386
    31. Stimulation of 5-HT3 serotonin receptor augments extrapyramidal motor symptoms; antagonism of this receptor alleviates extrapyramidal motor disorder symptoms. PMID: 20813116
    32. BDNF facilitates gamma oscillations in the hippocampus by attenuating signaling through 5-HT3 receptor PMID: 21298058
    33. Studies concluded that 5-HT3 receptor antagonists can reduce various opioid maladaptations. PMID: 21368652
    34. the relative positions of various residues in the ligand-binding site PMID: 20441748
    35. Both serotonin and granisetron bind to an AA binding site in the heteromeric 5-HT3AB receptor. PMID: 20409468
    36. A cation-pi interaction between serotonin and Trp-183 of the serotonin channel 5-HT3AR has been identified, precisely locating the ligand-binding site of this receptor. PMID: 12162741
    37. adaptive changes occur in the subunit composition of enteric 5-HT(3) receptors of 5-HT transporter -/- mice that are reflected in 5-HT(3) receptor affinity and desensitization PMID: 12388212
    38. species-dependent gating mechanism of 5-HT(3) receptors. PMID: 12457738
    39. serotonin transporter gene promoter variant was found in panic disorder. PMID: 12607224
    40. 5HT3A receptor function and trafficking is regulated by protein kinase c through an F-actin-dependent mechanism PMID: 12791692
    41. Results describe homology modeling of the N-terminal extracellular regions of human, mouse, and guinea pig 5-hydroxytryptamine type 3A receptors. PMID: 14626451
    42. The homopentameric 5-HT3A receptor is localized both to the plasma membrane and within intracellular compartments of living cells. PMID: 15009132
    43. hRIC-3 can act as a specific regulator of alpha7 and 5-HT3 expression at different levels by increasing the number of mature receptors and facilitating its transport to the membrane PMID: 15927954
    44. Residues F226 and Y234 of 5-hydroxytryptamine (serotonin) receptor are important for both 5-HT and mCPBG interactions PMID: 15966738
    45. results confirm the structure of the M2-M3 loop and the critical role of Pro 8* in the 5-HT3 receptor; in addition, they suggest that a molecular rearrangement at Pro 8* is the structural mechanism that opens the receptor pore PMID: 16281040
    46. the arginine 427 residue in the large cytoplasmic domain contributes to 5-HT3A receptor desensitization, possibly through forming an electrostatic interaction with its neighboring residues PMID: 16754678
    47. tested the hypothesis that loop F plays a significant role in conferring interspecies curare potency differences; two loop F residues make a significant contribution in determining curare potency at the 5-HT3A receptor PMID: 17260949
    48. These data show a role for the 5-HT(3A)-receptor subunit in the induction of behavioral sensitization to cocaine and suggest that the 5-HT(3A) molecular subunit modulates neurobehavioral adaptations to cocaine, which may underlie aspects of addiction. PMID: 17559417
    49. 5-HT mediates enhanced neurogenic contractions of the mouse bladder muscle by an action at 5-HT(3) receptors. The presence of the 5-HT(3) receptor was confirmed by the expression of both 5-HT(3A) and 5-HT(3B) receptor subunits. PMID: 17607564
    50. Results imply that the M3M4 loop is not essential for 5-HT3A receptor assembly and function and suggest that the cytoplasmic domain may fold as an independent module from the transmembrane and extracellular domains. PMID: 18227272

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily, HTR3A sub-subfamily
  • 组织特异性：
    Brain, spinal cord, and heart.
  • 数据库链接：

    KEGG: mmu:15561

    STRING: 10090.ENSMUSP00000003826

    UniGene: Mm.4831