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  4. Recombinant Mouse C-C chemokine receptor type 2 (Ccr2)

Recombinant Mouse C-C chemokine receptor type 2 (Ccr2)

  • 货号：
    CSB-CF004841MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Ccr2; Cmkbr2; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2; JE/FIC receptor; MCP-1 receptor; CD antigen CD192
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-373
  • 氨基酸序列
    MEDNNMLPQFIHGILSTSHSLFTRSIQELDEGATTPYDYDDGEPCHKTSVKQIGAWILPP LYSLVFIFGFVGNMLVIIILIGCKKLKSMTDIYLLNLAISDLLFLLTLPFWAHYAANEWV FGNIMCKVFTGLYHIGYFGGIFFIILLTIDRYLAIVHAVFALKARTVTFGVITSVVTWVV AVFASLPGIIFTKSKQDDHHYTCGPYFTQLWKNFQTIMRNILSLILPLLVMVICYSGILH TLFRCRNEKKRHRAVRLIFAIMIVYFLFWTPYNIVLFLTTFQESLGMSNCVIDKHLDQAM QVTETLGMTHCCINPVIYAFVGEKFRRYLSIFFRKHIAKRLCKQCPVFYRETADRVSSTF TPSTGEQEVSVGL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key functional receptor for CCL2 but can also bind CCL7 and CCL12 chemokines. Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion. Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B. Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation. Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B. Mediates the recruitment of macrophages and monocytes to the injury site following brain injury.
  • 基因功能参考文献：
    1. developmental ethanol exposure caused permanent loss of spinal cord neurons and CCR2 signaling played an important role in ethanol neurotoxicity. PMID: 28778590
    2. NOX4 is induced in early alcoholic liver injury and regulates CCR2/CCL2 mRNA stability thereby promoting recruitment of inflammatory cells and production of proinflammatory cytokines. PMID: 28383062
    3. Ccl2-Ccr2 signaling recruits a distinct fetal microchimeric population that rescues delayed maternal wound healing. PMID: 28516946
    4. itration reduced the potential of CCL2 to stimulate monocyte migration in diffusion gradient chemotaxis assays (p < 0.05). This was consistent with a trend towards reduced affinity of the nitrated chemokine for its cognate receptor CCR2b. PMID: 28290520
    5. Using the Theiler's virus model of encephalitis in C57BL/6 mice study shows that CCR2 as well as CX3CR1 plays a key role in the accumulation of myeloid cells in the CNS and activation of hippocampal myeloid cells upon infection. PMID: 30181265
    6. Enhanced bacterial clearance in CCR2(-/-) mice correlated with reduced numbers of IMs in spleens and increased numbers of neutrophils in livers. PMID: 29263104
    7. Inflammatory CCR2(+) cells that reach the injured kidney at initial stages. PMID: 28168553
    8. ANG II is up-regulated in serum and heart tissues of mice with EAM and that ANG II significantly drives monocyte/macrophage infiltration through the C-C chemokine receptor 2/5 (CCR2/5) axis. PMID: 29052263
    9. this study shows that CCR-2/TLR-2 triggered signaling in murine peritoneal macrophages intensifies bacterial (Staphylococcus aureus) killing by reactive oxygen species through TNF-R1 PMID: 28736159
    10. this paper shows CCR2(-) and CCR2(+) corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion PMID: 28120849
    11. this study shows that the chemokine receptor CCR2 maintains plasmacytoid dendritic cell homeostasis PMID: 29106985
    12. beta-arrestin2/AP-1-dependent beta2AR signaling has a critical role in the regulation of CCR2 expression and recruitment of leukocytes to the heart following injury PMID: 27956622
    13. Although CCR2 and CX3CR1 may synergistically impact inflammatory phenotypes, their joint deficiency did not influence the metabolic effects of a 45% high-fat diet-induced obesity in these model conditions. PMID: 28650582
    14. Chemokine receptor 2 (CCR2(+)) monocytes invade the hippocampus between 1 and 3 d after SE. In contrast, only an occasional CD3(+) T lymphocyte was encountered 3 d after SE. The initial cellular sources of the chemokine CCL2, a ligand for CCR2, included perivascular macrophages and microglia. The induction of the proinflammatory cytokine IL-1beta was greater in FACS-isolated microglia than in brain-invading monocytes PMID: 27601660
    15. TLR9-driven inflammation induced a Ccr2-independent expansion of functionally enhanced extramedullary myeloid progenitors that correlated with the peripheral accumulation of monocytes in both wild-type and Ccr2(-/-) mice. PMID: 27621476
    16. Suggest role for CCR2 expression in podocytes to mediate diabetic renal injury, independent of monocyte/macrophage recruitment. PMID: 27914709
    17. CCL2 recruits T cells into the brain in a CCR2-independent manner. PMID: 28836736
    18. in vitro and in vivo results show that CCR2 intrinsically mediates the expression of inflammatory T cell cytokines, and its absence on T cells results in attenuated colitis progression PMID: 28507030
    19. tumor-promoting role for CCL2 acting through CCR2 on the tumor microenvironment PMID: 27820834
    20. CCR2 expression on bone marrow-derived cells, likely macrophages, is essential to muscle regeneration independent of TLR signaling, aging, and sex. PMID: 27531927
    21. we demonstrated that CX3CR1(Lo)CCR2(Hi) monocytes were recruited to the GBM, where they transitioned to CX3CR1(Hi)CCR2(Lo) macrophages and CX3CR1(Hi)CCR2(-) microglia-like cells. Infiltrating macrophages/monocytes constituted approximately 85% of the total TAM population, with resident microglia accounting for the approximately 15% remaining. PMID: 28235764
    22. we characterized a novel regulatory network in macrophages, in which CCR2 activates PI3K-AKT-mTOR signaling to mediate HIF-1alpha expression, which then drives VEGF-C expression to promote lymphangiogenesis. PMID: 28627412
    23. The findings suggest that CCR2 deficiency does not provide a sustained benefit and that Ly6C(low) macrophages may contribute to the progressive fibrosis and dysfunction of mdx(5cv) diaphragm. PMID: 27655900
    24. In Ccr2(-/-) mice, a damaged microglia recruitment was shown in retina and this could protect the visual function in electroretinogram and alleviate the photoreceptor apoptosis. PMID: 27831552
    25. This study establishes ECL1i as the first allosteric inhibitor of CCR2 with functional selectivity. PMID: 26979087
    26. the individual and combined actions of CCL2/CCR2 and CX3CL1/CX3CR1 in hypoxia-induced pulmonary hypertension in mice, is reported. PMID: 28125278
    27. Gas6 specifically promotes the recruitment of inflammatory CCR2(hi)CX3CR1(lo) monocytes through the regulation of both CCR2 and CCL2 during deep venous thrombosis. PMID: 28450294
    28. macrophage-specific chemokine (C-C Motif) receptor 2 (Ccr2) knockout (M-Ccr2KO) and intestinal epithelial cell-specific tamoxifen-inducible Ccl2 knockout (Vil-Ccl2KO) mice, were studied. PMID: 27508875
    29. Kupffer cells secrete CCL2 to promote CCR2-expressing neutrophil recruitment from the bone marrow and subsequent infiltration into the liver during ischemia/reperfusion injury. These findings reveal a novel pro-inflammatory role of cell-mediated CCL2-CCR2 interactions during this sterile insult. PMID: 27229110
    30. CCR2 is dispensable for at least some forms of lung fibrosis and suggest that anti-CCR2 therapies may be ineffective in IL-17-mediated fibrotic disease. PMID: 27448666
    31. this study shows that that CCR2 signaling is required for monocyte recruitment during Clostridium difficile colitis PMID: 27995603
    32. this study shows that CCR2-deficient mice display enhanced resistance to Japanese encephalitis virus PMID: 27260136
    33. this study shows that CCR-2 signaling is involved in the killing of Staphylococcus aureus by murine bone marrow cells PMID: 28409543
    34. The results indicate that CCR2 plays an essential role in Helicobacter pylori-induced immune tolerance and shed light on a novel mechanism of CCR2-dependent dendritic cell Treg cell induction, which appears to be important in maintaining mucosal homeostasis during Helicobacter pylori infection. PMID: 27933701
    35. CCR2(-/-) resident cardiac Macrophage favor a more antiinflammatory phenotype relative to WT controls PMID: 27327107
    36. Endothelial VCAM-1 targeted delivery of the CCR2 antagonist resulted in inhibition of pulmonary metastases both in a murine (MC-38GFP cells) and a human xenograft (patient-derived cells) model. PMID: 26522070
    37. To interrogate the role of CCR2 activation on Nf1(+/-) neointima formation, s induced neointima formation by carotid artery ligation in Nf1(+/-) and WT mice with genetic deletion of either MCP1 or CCR2. Loss of MCP-1 or CCR2 expression effectively inhibited Nf1(+/-) neointima formation and reduced macrophage content in the arterial wall. PMID: 26740548
    38. involved in Staphylococcus aureus-induced MCP-1 production via NF-kB or p38-MAPK signaling PMID: 26616292
    39. CCL2 and its receptor CCR2 are important for the formation of osteoclasts and foreign body giant cells. PMID: 26205994
    40. Disruption of CCR2 enhanced tau pathology and reduced cavity volume in mild traumatic brain injury. PMID: 26634348
    41. In the absence of the rd8 allele, deficiency of CCR2 and CX3CL1 in mice leads to a mild form of retinal degeneration which is associated with the recruitment of macrophages, particularly to the subretinal space. This model enables to assess consequences of perturbed chemokine signaling, but it does not recapitulate cardinal age-related macular degeneration features. PMID: 26670885
    42. This study introduces a model for investigating the coronary collateral circulation in mice, demonstrates that neo-collaterals form rapidly after coronary occlusion and finds that MCPCCR2-mediated recruitment of myeloid cells is required for this process PMID: 26254180
    43. The results indicate that AMPK is a negative regulator of Ccr2 expression in RAW264.7 macrophages, and that the mechanism of action of AMPK inhibition of Ccr2 is mediated, in part, through the NF-kappaB pathway. PMID: 26799633
    44. results demonstrate the central role CCR2 plays in mediating leukocyte mobilization from the BM, and suggest a role for CCR2 inhibition in managing monocytes/macrophages-mediated chronic inflammatory conditions PMID: 26206182
    45. cellular fibrin uptake was diminished by elimination of CCR2-expressing cells. PMID: 26647393
    46. CCL2/CCR2 signaling pathway may provide a novel therapeutic target to prevent renal disease progression in patients with renovascular hypertension. PMID: 26661648
    47. In Ccr2(-/-) mice, in which monocyte-derived macrophage infiltration is impaired, silica administration induced diffuse PF with loose nodule formation and greater activation of tissue cells. PMID: 26456580
    48. IL-23/IL-1/IFNgamma/TNFalpha/T-bet/Eomesodermin-driven circuit drives GM-CSF/IFNgamma-producing Th17 cell development. PMID: 26511769
    49. a Th1 response in the modulation of Th2 allergic inflammation, our findings also attribute an important role to the innate response mediated by TLR9, associated with the recruitment of CCR2-dependent monocytes. PMID: 25944185
    50. the present study shows that sildenafil modulates inflammation, with the involvement of MMP-9, MCP-1, and CCR-2, and also contributes to myelin repair. PMID: 26515692

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Epressed in mature thymocytes. Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines.
  • 数据库链接：

    KEGG: mmu:12772

    STRING: 10090.ENSMUSP00000049909

    UniGene: Mm.6272