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  4. Recombinant Mouse Ceramide glucosyltransferase (Ugcg)

Recombinant Mouse Ceramide glucosyltransferase (Ugcg)

  • 中文名称：
    小鼠Ugcg重组蛋白
  • 货号：
    CSB-CF025564MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Ugcg
  • Uniprot No.：
  • 别名：
    Ugcg; Ceramide glucosyltransferase; GLCT-1; Glucosylceramide synthase; GCS; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-394
  • 氨基酸序列
    MALLDLAQEGMALFGFVLFVVLWLMHFMSIIYTRLHLNKKATDKQPYSKLPGVSLLKPLK GVDPNLINNLETFFELDYPKYEVLLCVQDHDDPAIDVCKKLLGKYPNVDARLFIGGKKVG INPKINNLMPAYEVAKYDLIWICDSGIRVIPDTLTDMVNQMTEKVGLVHGLPYVADRQGF AATLEQVYFGTSHPRSYISANVTGFKCVTGMSCLMRKDVLDQAGGLIAFAQYIAEDYFMA KAIADRGWRFSMSTQVAMQNSGSYSISQFQSRMIRWTKLRINMLPATIICEPISECFVAS LIIGWAAHHVFRWDIMVFFMCHCLAWFIFDYIQLRGVQGGTLCFSKLDYAVAWFIRESMT IYIFLSALWDPTISWRTGRYRLRCGGTAEEILDV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes at the cytosolic surface of the Golgi, the initial step of the glucosylceramide-based glycosphingolipid/GSL synthetic pathway, the transfer of glucose from UDP-glucose to ceramide to produce glucosylceramide/GlcCer. Glucosylceramide is the core component of glycosphingolipids/GSLs, amphipathic molecules consisting of a ceramide lipid moiety embedded in the outer leaflet of the membrane, linked to one of hundreds of different externally oriented oligosaccharide structures. Glycosphingolipids are essential components of membrane microdomains that mediate membrane trafficking and signal transduction. They are implicated in many fundamental cellular processes, including growth, differentiation, migration, morphogenesis, cell-to-cell and cell-to-matrix interactions. They are required for instance in the proper development and functioning of the nervous system. As an example of their role in signal transduction, they regulate the leptin receptor/LEPR in the leptin-mediated signaling pathway. They also play an important role in the establishment of the skin barrier regulating keratinocyte differentiation and the proper assembly of the cornified envelope. The biosynthesis of GSLs is also required for the proper intestinal endocytic uptake of nutritional lipids.
  • 基因功能参考文献：
    1. we report the development of a novel, orally available glucosylceramide synthase inhibitor (Genz-682452) with pharmacological and safety profiles that have potential for treating Fabry disease. PMID: 25938659
    2. These results suggest that neuronal glucosylceramide synthase expression modulates mediobasal hypothalamus insulin signaling and white adipose tissue function in fasted mice. PMID: 26038579
    3. we measured the expression and activities of Pgp and GCS, UDP-glucose levels, cellular uptake of C12-NBD-ceramide (a fluorescent analogue of ceramide) and ceramide-induced cell death in S and R cells. PMID: 25964538
    4. Data indicate that verexpression of glucosylceramide synthase in myotubes induces glucosylceramide but enhances insulin signaling. PMID: 24214972
    5. Data indicate that mice fed D- threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP), an inhibitor of glucosylceramide synthase and lactosylceramide synthase showed marked reduction in tumor volume. PMID: 23671696
    6. The present work demonstrates that hypothalamic integration of metabolic signals requires neuronal expression of glucosylceramide synthase (GCS; UDP-glucose:ceramide glucosyltransferase). PMID: 23554574
    7. Data indicate that mice with cerebroside sulfotransferases (Cst) and UDP-glucose:ceramide glucosyltransferase (Ugcg)/Cst deficiency had lower ammonium excretion. PMID: 23716689
    8. glycosphingolipids in hepatocytes are not essential for sterol, glucose, or lipoprotein metabolism. Ugcg inhibitors exert their effect on hepatocytes either independently of GSL or mediated by other (liver) cell types. PMID: 20432257
    9. Ugcg and Ugt8a deficient oligodendroglial did not exhibit any phenotypic or myelin structural abnormalities; abundant and structurally intact myelin can form in their absence PMID: 19705459
    10. Shortly after birth UgcG deficient mice showed dysfunction of cerebellum and peripheral nerves, associated with structural defects PMID: 17867597

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  • 亚细胞定位：
    Golgi apparatus membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Glycosyltransferase 2 family
  • 数据库链接：

    KEGG: mmu:22234

    STRING: 10090.ENSMUSP00000030074

    UniGene: Mm.198803