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  4. Recombinant Mouse Corticotropin-releasing factor receptor 2 (Crhr2)

Recombinant Mouse Corticotropin-releasing factor receptor 2 (Crhr2)

  • 中文名称：
    Recombinant Mouse Corticotropin-releasing factor receptor 2(Crhr2)
  • 货号：
    CSB-CF730654MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Crhr2; Crf2r; Corticotropin-releasing factor receptor 2; CRF-R-2; CRF-R2; CRFR-2; CRF-RB; Corticotropin-releasing hormone receptor 2; CRH-R-2; CRH-R2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-431
  • 氨基酸序列
    VAQPGQAPQDQPLWTLLEQYCHRTTIGNFSGPYTYCNTTLDQIGTCWPQSAPGALVERPC PEYFNGIKYNTTRNAYRECLENGTWASRVNYSHCEPILDDKQRKYDLHYRIALIVNYLGH CVSVVALVAAFLLFLVLRSIRCLRNVIHWNLITTFILRNIAWFLLQLIDHEVHEGNEVWC RCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTEHLRKWLFLFIGWCIPCPIIIAWAV GKLYYENEQCWFGKEAGDLVDYIYQGPVMLVLLINFVFLFNIVRILMTKLRASTTSETIQ YRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIVFIYFNSFLQSFQGFFVSVFYCFFNG EVRAALRKRWHRWQDHHALRVPVARAMSIPTSPTRISFHSIKQTAAV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.
  • 基因功能参考文献：
    1. CRFR2-expressing neurons within the posterior bed nucleus of the stria terminalis (pBNST) send dense inhibitory projections to other stress-related brain regions. Local CRFR2 activation by urocortin 3 depolarized the cells, increased the neuronal input resistance and increased firing of action potentials. Optogenetic activation of CRFR2 neurons in the pBNST decreased anxiety and attenuated stress responses. PMID: 27550842
    2. Urocortin 2 acts on gonadotrophs expressing the corticotropin-releasing factor type 2 receptor, and inhibits the production of gonadotropins in the pituitary gonadotropic tumor cells. PMID: 28851616
    3. Results indicate that corticotropin releasing hormone receptor 2 (Crhr2) is highly expressed in cardiomyocytes, and cardiomyocyte-specific deletion of Crhr2 protected mice from pressure overload-induced cardiac dysfunction. PMID: 28550160
    4. findings collectively suggest that the MeA Ucn3-CRF-R2 system modulates the ability of mice to cope with social challenges PMID: 27428651
    5. Study suggests that selective CRF2 receptor agonists urocortin 2 and 3 could be used as a therapy in nicotine addiction. PMID: 27693397
    6. results indicate that long-term UVA eye irradiation led to increased gp91phox-derived ROS in the brain and the increased expression of urocortin 2 and CRHR type 2, resulting in photoaging; however, further studies are needed to confirm these findings PMID: 26648475
    7. Crhr2(-/-) mice showed increased mortality in the dextran sodium sulfate healing protocol PMID: 26597886
    8. the inhibitory effect of CRFR2 signaling on insulin action is mediated by cAMP in a mammalian target of rapamycin-dependent manner. PMID: 25875045
    9. Data suggest that signal transduction via ghrelin and Crhr2 plays role in regulation of muscle glucose metabolism; Ghsr1a (ghrelin receptor type 1A) was not detected in mouse myoblast cell line (C2C12) investigated. PMID: 23804489
    10. The receptors' binding specificity and structural studies were performed using full-length human CRFR1alpha and mouse CRFR2beta as well as fragments lacking the N-terminal extracellular domain. PMID: 24465390
    11. estrogens act on ERalpha to up-regulate CRHR2 expression in cardiomyocytes. PMID: 24035863
    12. These results provide initial evidence of a gender-independent and specific role for the CRF2 receptor in the motivational effects of opiate withdrawal. PMID: 23707482
    13. stress-induced anorexia in A(y)/a mice may be associated with increased anorectic signals mediated by CRFR2 in the hypothalamus PMID: 23834894
    14. Brain CRF2 receptors do not tonically promote anxiogenic-like behavior. PMID: 24015170
    15. Corticotropin-releasing factor receptor 2 mediates sex-specific cellular stress responses. PMID: 23835907
    16. CRF2 receptor-deficient mice lack the dysphoria-like and the anhedonia-like states of opiate withdrawal. PMID: 21946917
    17. data show that CRFR2 mRNA upregulation in the BNST coincides with PTSD-like symptoms Theyfurther show that knockdown of CRFR2 in the posterior medial BNST of adult mice reduces susceptibility to PTSD-like behaviors. PMID: 22593059
    18. The CRF peptide reduces both apoptotic and necrotic cell death in cardiac myocytes subjected to lethal ischemic induced stress through activation of PKA and PKC dependent signaling pathways downstream of CRFR2. PMID: 22209828
    19. CRF(1) agonists, Ucn 1 and stressin(1) -A, reduced feeding and induced interoceptive stress, whereas Ucn 2 potently suppressed feeding via a CRF(2) -dependent mechanism without eliciting malaise PMID: 21627635
    20. Type 2 corticotropin-releasing factor receptor in the ventromedial nucleus of hypothalamus is critical in regulating feeding and lipid metabolism in white adipose tissue. PMID: 22067315
    21. Urocortin 3 acting on CRFR2 in skeletal muscle of Ucn3(+) mice results in a metabolic phenotype, with lean body composition and protection against diet-induced obesity and hyperglycaemia. PMID: 21667214
    22. Data suggest that UCN regulates osteoclast resorption by suppressing osteoclast maturation/function via suppression of constitutively active cation channel (CRFR2beta) with properties of canonical transient receptor potential 1 (TRPC1) channel. PMID: 22083217
    23. CRH-R2 participates in sleep-wake regulation via an interaction with the activated immune system PMID: 21704697
    24. CRH-R2 inhibition attenuates stress-induced bacterial growth and significantly prevents severe sepsis. PMID: 21774994
    25. The exclusive expression of CRFR2, and not CRFR1, in mature skeletal muscle tissue. PMID: 21084379
    26. CRF(2) activation appears to mitigate isolation rearing effects on exploratory behavior PMID: 20466421
    27. The results of this study suggested a novel role for UCN3 and CRH-R2 related to the processing of social cues and to the establishment of social memories. PMID: 20610744
    28. Wild type (WT) and CRFR2-KO, but not CRFR1-knockout, mice lost weight during restraint stress PMID: 20392192
    29. less abundant than CRHR1 in cerebral microvessels and less prone to changes with development PMID: 20032050
    30. It was also shown that the activation of CRFR2 could inhibit p38 and Akt phosphorylation to suppress the secretion of VEGF in SCLC cells. PMID: 19968503
    31. supresses vascularization PMID: 12032352
    32. analysis of circadian heartbeat interval fluctuations indicates that CRFR2-deficiency in mice does not interfere with the dynamical mechanisms underlying the control of heart rate PMID: 12637030
    33. CRFR2 plays a critical role in regulation of energy expenditure and is important for responses to homeostatic challenges. PMID: 12746321
    34. involvement of CRFR2 in heart rate dynamics upon pharmacological stimulation but demonstrate that CRFR2 is not involved in baseline heart rate regulation and stress-mediated modulation of heart rate responses. PMID: 12786991
    35. Evidence is provided for a specific role for CRF receptor type 2 in modulation of dopaminergic neurons of the ventral tegmental area. PMID: 12895416
    36. Corticotropin-releasing factor receptor 2 has a role in fear conditioning and stress-enhanced fear conditioning. PMID: 14673008
    37. two ligand-selective CRF2 receptor splice variants were found in mice and the CRF2(a) receptor is the predominant central CRF2 receptor in the mouse. PMID: 15256278
    38. The role of CRF2 in the defensive startle response in mice. PMID: 15269266
    39. analysis of corticotropin-releasing factor receptor type 2alpha gene PMID: 15514029
    40. identified a cDNA from mouse brain encoding a splice variant, soluble CRFR2alpha (sCRFR2alpha), in which exon 6 is deleted from the gene encoding CRFR2alpha PMID: 15701705
    41. Differences in stress sensitivity and the overproduction of CRF of the knockout (KO) mice specifically affects maternal, but not intermale aggression. PMID: 15836912
    42. Corticotropin releasing factor over-expression in the mouse brain is associated with up-regulation of CRF2 mRNA. PMID: 16423327
    43. crucial role CRFR2 plays in the regulation of regional tissue thermogenesis and adaptive physiology PMID: 16492754
    44. Collectively, these results point toward a role for CRF(2) pathways in neural circuits that subserve stress-coping behaviors. PMID: 16507004
    45. CRF(2) agonists appear to have both bronchorelaxant and anti-inflammatory activities and may represent an interesting therapeutic approach to the treatment of inflammatory lung diseases. PMID: 16855006
    46. CRHR2 mediates intestinal inflammatory responses via release of proinflammatory mediators at the colonocyte level PMID: 16920976
    47. CRF-R2alpha is present in the cerebellum and functions in circuits that modulate the firing rate of Purkinje cells PMID: 16955482
    48. These results demonstrate for the first time expression of CRF-R2 in the GT1-7 cells and suggest that CRF may directly regulate GnRH gene expression, an effect mediated, at least in part, by CRF-R2. PMID: 17175507
    49. Crfr2 is expressed in mouse colon and is overexpressed in altered behavioral response to superimposed mild stressors. PMID: 17194724
    50. Stress reduced CRHr in BALB/cByJ mice. In C57BL/6ByJ mice acute stress increased CRHr in portions of the orbital frontal cortex, whereas chronic stress reduced CRHr. CRH(2) expression was unaltered by the stressor in both strains. PMID: 17517441

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 2 family
  • 组织特异性：
    Highly expressed in the heart. Also expressed in lungs, skeletal muscle, gastrointestinal tract, epididymis, and brain.
  • 数据库链接：

    KEGG: mmu:12922

    STRING: 10090.ENSMUSP00000003568

    UniGene: Mm.236081