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  4. Recombinant Mouse Diacylglycerol O-acyltransferase 2 (Dgat2)

Recombinant Mouse Diacylglycerol O-acyltransferase 2 (Dgat2)

  • 中文名称：
    小鼠Dgat2重组蛋白
  • 货号：
    CSB-CF866760MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Dgat2
  • Uniprot No.：
  • 别名：
    Dgat2; Diacylglycerol O-acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase; ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-388
  • 氨基酸序列
    MKTLIAAYSGVLRGERRAEAARSENKNKGSALSREGSGRWGTGSSILSALQDIFSVTWLN RSKVEKQLQVISVLQWVLSFLVLGVACSVILMYTFCTDCWLIAVLYFTWLAFDWNTPKKG GRRSQWVRNWAVWRYFRDYFPIQLVKTHNLLTTRNYIFGYHPHGIMGLGAFCNFSTEATE VSKKFPGIRPYLATLAGNFRMPVLREYLMSGGICPVNRDTIDYLLSKNGSGNAIIIVVGG AAESLSSMPGKNAVTLKNRKGFVKLALRHGADLVPTYSFGENEVYKQVIFEEGSWGRWVQ KKFQKYIGFAPCIFHGRGLFSSDTWGLVPYSKPITTVVGEPITVPKLEHPTQKDIDLYHA MYMEALVKLFDNHKTKFGLPETEVLEVN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG).
  • 基因功能参考文献：
    1. DGAT1 does not contribute to this pathway, but uses exogenous FA and glycerol to synthesize a functionally distinct pool of TAG to which DGAT2 also contributes...in b3-agonist activated brown adipocytes, DGAT2 specifically enables channelling of de novo synthesized FA into a rapidly mobilized pool of TAG, which is simultaneously hydrolyzed to provide substrates for mitochondrial fatty acid oxidation. PMID: 27836993
    2. these results support a model where Dgat1 and Dgat2 function coordinately to regulate the process of dietary fat absorption by preferentially synthesizing TAG for incorporation into distinct subcellular TAG pools in enterocytes. PMID: 28249764
    3. when intestinal DGAT2 is present in excess, mRNA levels of genes involved in fatty acid oxidation were reduced. This result suggests that reduced fatty acid oxidation may contribute to increased TG secretion by overexpression of DGAT2 in intestine PMID: 23643496
    4. role of Mtp and DGAT2 in hepatic steatosis PMID: 22355095
    5. DGAT2 is capable of catalyzing TG synthesis and promote its storage in cytosolic lipid droplets independent of its localization in the ER. PMID: 21680734
    6. DAG-induced PKCepsilon activation plays a major role in nonalcoholic fatty liver disease (NAFLD)-associated hepatic insulin resistance. PMID: 21436037
    7. Findings show that DGAT1 and DGAT2 account for the vast majority of TG synthesis in mice, and DGAT function is required for LDs in adipocytes, but not in all cell types. PMID: 21317108
    8. upregulation of DGAT2 plays an important role in the pathogenesis of alcoholic fatty liver disease PMID: 20739640
    9. the increased rate of hepatic TAG secretion and intracellular accumulation in ob/ob mice is accompanied by parallel increases in the activities of both DGAT I and DGAT II PMID: 12032764
    10. role in assembly of de novo synthesized fatty acids into VLDL particles in the liver PMID: 12413942
    11. DGAT2 expression is increased in diabetic mice. PMID: 14521909
    12. plays a fundamental role in mammalian triglyceride synthesis and is required for survival PMID: 14668353
    13. leptin regulates adipocyte size by altering expression patterns of DGAT via central nervous system to determine the levels of triglyceride synthesis. PMID: 15550388
    14. DGAT1 but not DGAT2 has a role in VLDL synthesis; increased plasma VLDL concentrations may promote obesity, whereas increased DGAT2 activity has a role in steatosis PMID: 15797871
    15. Hepatic DGAT2 overexpression resulted in 2.4-fold increases in the triglyceride content of liver PMID: 16877777
    16. diacylglycerol acyltransferase 2 expression is regulated by CAAT/enhancer-binding protein beta (C/EBPbeta) and C/EBPalpha during adipogenesis PMID: 17504763
    17. These findings indicate that the majority of triglyceride destined for secretion by liver is synthesized by DGAT2. PMID: 18252207
    18. Consistent with pathways of hepatic triglyceride synthesis, expression of diacylglycerol acyltransferase-1 and -2 was increased, as were delta-5- and delta-6- fatty acid desaturase mRNA levels. PMID: 18280001
    19. DGAT2, an ER-resident transmembrane domain-containing enzyme, is also found in mitochondria-associated membranes, where its N terminus may promote its association with mitochondria. PMID: 19049983

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Lipid droplet. Cytoplasm, perinuclear region.
  • 蛋白家族：
    Diacylglycerol acyltransferase family
  • 组织特异性：
    Predominantly expressed in liver. Also expressed in testis.
  • 数据库链接：

    KEGG: mmu:67800

    STRING: 10090.ENSMUSP00000033001

    UniGene: Mm.180189