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  4. Recombinant Mouse E3 ubiquitin-protein ligase MARCH1 (41334)

Recombinant Mouse E3 ubiquitin-protein ligase MARCH1 (41334)

  • 货号：
    CSB-CF740693MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    March1
  • Uniprot No.：
  • 别名：
    Marchf1; March1; E3 ubiquitin-protein ligase MARCHF1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; MARCH-I; RING-type E3 ubiquitin transferase MARCHF1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-289
  • 氨基酸序列
    MLGWCEAIARNPHRIPNTTRTPETSGDVADASQTSTLNEKSPGRSASRSSNISKASSPTT GTAPRSQSRLSVCPSTQDICRICHCEGDEESPLITPCRCTGTLRFVHQSCLHQWIKSSDT RCCELCKYDFIMETKLKPLRKWEKLQMTTSERRKIFCSVTFHVIAVTCVVWSLYVLIDRT AEEIKQGNDNGVLEWPFWTKLVVVAIGFTGGLVFMYVQCKVYVQLWRRLKAYNRVIFVQN CPDTANKLEKNFPCNVNTEIKDAVVVPVPQTGSNTLPTAEGAPPEVIPV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.
  • 基因功能参考文献：
    1. Study shows march1-dependent modulation of donor MHC II on CD103(+) dendritic cells mitigates alloimmunity PMID: 30154416
    2. these results demonstrate a role of March1 E3 ubiquitin ligase in modulating allergic responses PMID: 29854835
    3. March1 loss-of-function enhances, and March1 overexpression impairs, hepatic insulin sensitivity in mice. PMID: 27577745
    4. We conclude that MARCH1 exerts MHCII-independent effects that regulate the innate arm of immunity PMID: 27940660
    5. March-I ubiquitination prevents MHC class II recycling and promotes MHC class II turnover in antigen-presenting cells PMID: 26240324
    6. IL-10 stimulates expression of the E3 ubiquitin ligase March-I in activated macrophages, thereby down-regulating MHC-II, CD86, and antigen presentation to CD4 T cells. PMID: 26408197
    7. Dendritic cells co-cultivated with antigen-specific induced Tregs expressed high levels of MARCH1. A major suppressive mechanism of DC function by iTregs is secondary to the effects of IL-10 on MARCH1 & CD83 expression. PMID: 24218453
    8. DCs deficient in MARCH1 or MHCII ubiquitination both failed to generate antigen-specific T reg cells in vivo and in vitro. PMID: 23712430
    9. aberrant degradation of spleen DCs MARCH1-mediated ubiquitinated proteins is involved during the earliest stage of MODS development. PMID: 23305794
    10. IL-10 can have opposite effects on MARCH1 regulation in different cell types. PMID: 22503116
    11. Ubiquitin-mediated regulation of CD86 protein expression by the ubiquitin ligase membrane-associated RING-CH-1 (MARCH1). PMID: 21896490
    12. found that the transmembrane domain of CD83 enhances MHC class II and CD86 expression by blocking MHC class II association with the ubiquitin ligase MARCH1 PMID: 21220452
    13. Selective ubiquitination of MHC-II in immature dendritic cells by the E3 ubiquitin ligase March-I results in the selective degradation of internalized pMHC-II. PMID: 21059907
    14. The function of MHC II in B cells is regulated through ubiquitination by MARCH-I. PMID: 17255932
    15. MARCH1 expression is regulated at a posttranscriptional level by trafficking within the endolysosomal pathway where MARCH1 is proteolyzed. PMID: 19880452
    16. MARCH-I may function as a housekeeper of conventional dendritic cell in the steady state. PMID: 19917682

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  • 亚细胞定位：
    Cytoplasmic vesicle membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
  • 数据库链接：

    KEGG: mmu:72925

    STRING: 10090.ENSMUSP00000072302

    UniGene: Mm.380457