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  4. Recombinant Mouse Inward rectifier potassium channel 2 (Kcnj2)

Recombinant Mouse Inward rectifier potassium channel 2 (Kcnj2)

  • 中文名称：
    小鼠Kcnj2重组蛋白
  • 货号：
    CSB-CF012055MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Kcnj2
  • Uniprot No.：
  • 别名：
    Kcnj2; Irk1; Inward rectifier potassium channel 2; Inward rectifier K(+ channel Kir2.1; IRK-1; Potassium channel, inwardly rectifying subfamily J member 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-428
  • 氨基酸序列
    GSVRTNRYSIVSSEEDGMKLATMAVANGFGNGKSKVHTRQQCRSRFVKKDGHCNVQFIN VGEKGQRYLADIFTTCVDIRWRWMLVIFCLAFVLSWLFFGCVFWLIALLHGDLDTSKVSK ACVSEVNSFTAAFLFSIETQTTIGYGFRCVTDECPIAVFMVVFQSIVGCIIDAFIIGAVM AKMAKPKKRNETLVFSHNAVIAMRDGKLCLMWRVGNLRKSHLVEAHVRAQLLKSRITSEG EYIPLDQIDINVGFDSGIDRIFLVSPITIVHEIDEDSPLYDLSKQDIDNADFEIVVILEG MVEATAMTTQCRSSYLANEILWGHRYEPVLFEEKHYYKVDYSRFHKTYEVPNTPLCSARD LAEKKYILSNANSFCYENEVALTSKEEEEDSENGVPESTSTDSPPGIDLHNQASVPLEPR PLRRESEI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
  • 基因功能参考文献：
    1. Cellular electrophysiology assays of mouse Kir2.1 and human Kir2.2 indicated that, consistent with simulations, the Leu residue increased the channel responses to phosphatidylinositol diphosphate (PIP2) through increased binding affinity and faster activation kinetics, and the deactivation kinetics decreased upon PIP2 inhibition. PMID: 26520451
    2. Our results support the concept that endothelial cell Kir2 channels boost vasodilatory signals that are generated by Ca(2+) -dependent activation of IK and SK channels. PMID: 26840527
    3. Results suggest that a promyogenic cell adhesion molecule Cdo signaling is critical for Inward rectifier potassium channel Kir2.1 activities in the induction of myogenic differentiation. PMID: 27380411
    4. The data suggest that microglial Kir2.1 channels may represent novel therapeutic targets to inhibit excessive reactive oxygen species production by primed microglia in brain pathology. PMID: 27598576
    5. Three pairs of weak interactions precisely regulate the G-loop gate of Kir2.1 channel. PMID: 27699887
    6. Suggest that Kir2.1 channels, in part, account for hyperpolarization and associated absence of tone in urinary bladder arterioles. PMID: 28148533
    7. This finding represents the first functional evidence for a significant role of the dystrophin-associated protein complex in the regulation of Kir2.x channels. PMID: 27560040
    8. Intracellular Mg(2+) and SPM therefore may have a synergistic action on the pore-blocking effect, presumably via prohibition of the outward exit of the higher-affinity blocking SPM by the lower-affinity Mg(2+). PMID: 26869275
    9. Consistent with a role of the K(+) current in amplifying the sensory response, entry of protons through the Zn(2+)-sensitive conductance produces a transient block of the KIR2.1 current. PMID: 26627720
    10. Mouse neutrophils express functional Kir2.1 channels from bone marrow and liver. PMID: 25472961
    11. A184R mutation in the inner end of the bundle crossing region of Kir2.1 not only abolishes the inward rectifying features of spermine block but also tends to close the channel pore. PMID: 24633623
    12. the present work demonstrates that a functional Kir2.1-like channel is expressed in lactating mouse mammary secretory cells. PMID: 24259419
    13. Kir2.1 mRNA levels were downregulated by LPS, and to a lesser extent by IL-4/IL-13. PMID: 24249731
    14. Report pentamidine analogue which specifically blocks cardiac KIR2.1 channel, lengthening action potential duration. PMID: 23625347
    15. The total Kcnj2 (gene for the Kir2.1 potassium channel) mRNA was reduced in Huntington disease skeletal muscle. PMID: 23671115
    16. miR-26 controls the expression of KCNJ2 and may have a role in atrial fibrillation PMID: 23543060
    17. Stronger expressions of SCN5a, SCN1b and Kir2.1 were observed in ventricular-like and atrial-like cells compared to that of pacemaker-like cardiomyocytes. PMID: 22348965
    18. The data of this study suggested that Kir2.1 is required for I(K1) in type II utricle hair cells. PMID: 22496522
    19. An inwardly rectifying K+ channel is required for patterning. PMID: 22949619
    20. These results demonstrate that three functional ion channel currents, I(KCa), I(Cl.vol), and I(Kir), are heterogeneously present in 3T3-L1 preadipocytes. I(KCa) and I(Cl.vol) participate in the regulation of cell proliferation. PMID: 21732368
    21. These results suggested that the blocking kinetics depends on driving force to produce driving force-dependent inward rectification when the equilibrium potential for potassium is altered by changing external potassium. PMID: 20962011
    22. HL-1 cardiac myocytes express a current that is characteristic of cardiac inward rectifier K(+) channels, and express K(ir)2.1 mRNA. PMID: 20568224
    23. Data suggest that the earlier-than-normal expression of Kir2.1 in hair cells inhibits their spontaneous activity required for spiral ganglion neuron survival and neurite growth. PMID: 20045730
    24. Modulation of the inward rectifier potassium channel IRK1 by the Ras signaling pathway PMID: 11809752
    25. AMPA receptor-mediated modulation of inward rectifier K+ channels in astrocytes of mouse hippocampus. PMID: 11906215
    26. K+ influxes from neuronal "sources" into glial cells are mediated mainly by strongly rectifying Kir channels (Kir2.1), expressed in glial membrane sites where direct glia-neuron interactions occur. PMID: 12203395
    27. Single and double mutations in the pore-forming M1-M2 segment of Kir2.1 characterize conductance states of the channel and demonstrate a novel functional role for M1-M2 as a critical structural determinant in adjustments of pore conformations. PMID: 12369836
    28. These findings can be accounted for by a model in which the negative charges at E224, E299 and D172 act in a concerted manner to coordinate the surface charge reduction and open channel components of polyamine block. PMID: 12740427
    29. significant role of IK1 in cardiac excitability is consistent with adverse effects of IK1 upregulation on cardiac electrical activity. PMID: 15271672
    30. Kv1.3 and Kir2.1 expression was impaired in brain during cancer cachexia PMID: 15304346
    31. a short sequence located in the N-terminal part of the linker between the two transmembrane segments is essential for high-affinity inhibition by TPN and variability in the region underlies the variation of TPN affinities among eukaryotic Kir channels PMID: 15311931
    32. These observations implicate E224 and E299 as allosteric modulators of a fast gate, located at the bundle crossing or below in Kir2.1 channels PMID: 15459242
    33. E153 contributes to the conductance properties of Kir2.1 channels by acting as a surface charge PMID: 15824191
    34. changes in these proteins and their modification may predispose striatal projection neurons to dysfunction and then degeneratation in Huntington disease PMID: 15880743
    35. The molecular interfaces formed between PSD95 PDZ domains and Kir2.1 tail involve regions outside the previously identified binding motif and may be important for additional channel-specific interactions with associating PDZ-containing proteins. PMID: 17437338
    36. mouse heart upregulation of IK1 is proarrhythmic PMID: 17546530
    37. Functional Kir2.1 ion channel currents are present in mouse bone marrow mesenchymal stem cells. PMID: 17699636
    38. We speculate that restricted dynamics & preferential Kir2.1 binding to PDZ2 are features that enable SAP97 to function as a scaffold protein, allowing other proteins to bind to other 2 PDZ domains in sufficient proximity to yield productive channelosomes. PMID: 18004877
    39. The interaction of PIP(2) with only one Kir2.1 subunit is sufficient for the channel to become available and to open to its full conductance state. Interaction with additional subunits exerts positive cooperativity at multiple levels. PMID: 18276733
    40. Results imply that Kir2.1 channels in astrocytes may be involved in buffering K(+) against accumulated extracellular K(+) caused by neuronal hyperexcitability under phathophysiological conditions. PMID: 18319624
    41. we describe the transcription profiles of the four HCN genes from embryonic stage E9.5 dpc to postnatal day 120 in the mouse. PMID: 19421833

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein. Membrane; Lipid-anchor.
  • 蛋白家族：
    Inward rectifier-type potassium channel (TC 1.A.2.1) family, KCNJ2 subfamily
  • 组织特异性：
    Prominently expressed in the central nervous system. Also found in other excitable tissues such as heart and skeletal muscle.
  • 数据库链接：

    KEGG: mmu:16518

    STRING: 10090.ENSMUSP00000037192

    UniGene: Mm.4951