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  4. Recombinant Mouse Lactosylceramide alpha-2,3-sialyltransferase (St3gal5)

Recombinant Mouse Lactosylceramide alpha-2,3-sialyltransferase (St3gal5)

  • 中文名称：
    小鼠St3gal5重组蛋白
  • 货号：
    CSB-CF022755MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    St3gal5
  • Uniprot No.：
  • 别名：
    St3gal5; GM3S; Siat9; Lactosylceramide alpha-2,3-sialyltransferase; CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase; Ganglioside GM3 synthase; ST3Gal V; ST3GalV; Sialyltransferase 9
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-414
  • 氨基酸序列
    MHTEAVGGAARRPQKLRSQAAAPACRAMPSEFTSAKLRSDCSRTSLQWYTRTQHKMRRPSLLIKDICKCTLVAFGVWLLYILILNYTAEECDMKRMHYVDPDRIKRAQSYAQEVLQKECRPRYAKTAMALLFEDRYSINLEPFVQKVPTASEAELKYDPPFGFRKFSSKVQSLLDMLPEHDFPEHLRAKACKRCVVVGNGGILHGLELGHALNQFDVVIRLNSAPVEGYSEHVGNKTTIRMTYPEGAPLSDVEYYANDLFVTVLFKSVDFKWLQAMVKNESLPFWVRLFFWKQVAEKVPLQPKHFRILNPVIIKETAFDILQYSEPQSRFWGHDKNIPTIGVIAVVLATHLCDEVSLAGFGYDLSQPRTPLHYFDSQCMGAMHWQVMHNVTTETKFLLKLLKEGVVEDLSGGIH
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Gangliosides GD1b and GT1b (derived from GM3) may serve as receptors for some C.botulinum neurotoxins (minimally types BoNT/A, B, C).; Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose (Gal) of glycosphingolipids forming gangliosides (important molecules involved in the regulation of multiple cellular processes, including cell proliferation and differentiation, apoptosis, embryogenesis, development, and oncogenesis). Mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1), although less preferentially than beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer (LacCer).
  • 基因功能参考文献：
    1. A new liver-specific c-type mRNA transcriptional variant of mouse ST3GAL5 protein has been identified. PMID: 28808804
    2. We used GM2/GD2 synthase (B4galnt1)-deficient mice to immunize by liposomes embedded with GD1alpha or acidic glycolipid fractions from brain of St3gal5-deficient mice. Specificities of established mAbs as analyzed by enzyme-linked immunosorbent assay and thin-layer chromatography-immunostaining were very high among various gangliosides. PMID: 27102283
    3. These studies establish ganglioside GM3 as a new candidate responsible for neuropathic pain and small fiber neuropathy in diabetes. PMID: 27590073
    4. R/K-based motif is necessary for the Golgi retention of ST3G5 . PMID: 26362868
    5. Functionally, the repression of St3gal5 suffices to elevate intercellular adhesion and expression of distinct junction-associated proteins, reminiscent of knockdown of Zeb1. PMID: 25643708
    6. ganglioside GM3 synthase has a role in siRNA-based spherical nucleic acid reversal of impaired wound healing in diabetic mice PMID: 25902507
    7. Results show that complete and partial deletion of the GM3 synthase gene exert distinct effects on the NP-C (Niemann-Pick disease Type C) phenotype. PMID: 24599001
    8. genes involved in the sphingolipids metabolism may be modifiers of cystogenesis, and suggest GM3 synthase as a new anti-cystic therapeutic target. PMID: 22563011
    9. Results suggest that complete, but not partial, inhibition of GM3 synthesis results in robust activation of an alternate pathway that may compensate for the complete absence of the products of GM3 synthase. PMID: 17604219
    10. the age at death of the npc1(-/-) mouse can be significantly influenced by many factors, including differences in strain background, other inactivating gene mutations in Siat9 and lxrbeta PMID: 18077828
    11. GM3 synthase silencing suppressed lung metastasis in murine breast cancer cells. The molecular mechanism that underlies GM3 synthase mediated migration and invasion was inhibition of the phosphoinositide-3 kinase/Akt pathway. PMID: 18171481
    12. Data show that M1-SAT-I and M3-SAT-I had a long half-life relative to M2-SAT-I. PMID: 19420140
    13. The defect of hearing ability of GM3 synthase null mice could be attributed to the functional disorganization of the organ of Corti, and the expression of gangliosides, especially GM3, during the maturation of the cochlea. PMID: 19470479
    14. zST3GalV-2 and mST3GalV are the enzymes responsible for the synthesis of GM4 in zebrafish and mice, respectively. PMID: 19542236

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  • 亚细胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Glycosyltransferase 29 family
  • 组织特异性：
    Mainly expressed in brain, and then testis, heart and liver, almost all tissues showed some levels of the gene expression.
  • 数据库链接：

    KEGG: mmu:20454

    STRING: 10090.ENSMUSP00000070414

    UniGene: Mm.38248