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  4. Recombinant Mouse Muscarinic acetylcholine receptor M3 (Chrm3)

Recombinant Mouse Muscarinic acetylcholine receptor M3 (Chrm3)

  • 货号：
    CSB-CF872136MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Chrm3; Chrm-3; Muscarinic acetylcholine receptor M3; Mm3 mAChR
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-589
  • 氨基酸序列
    MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVSQLDSYNISQTSGNFSSNDTSSDPLG GHTIWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVIS MNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRT TKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFY MPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEAENFVHPTGSSRSCSSYELQQQGTK RSSRRKYGGCHFWFTTKSWKPSAEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSET RAIYSIVLKLPGHSTILNSTKLPSSDNLQVPDKDLGTMDVERNAHKLQAQKSMDDRDNCQ KDFSKLPIQLESAVDTAKTSDTNSSVDKTTAALPLSFKEATLAKRFALKTRSQITKRKRM SLIKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNP VCYALCNKTFRTTFKMLLLCQCDKRKRRKQQYQQRQSVIFHKRVPEQAL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
  • 基因功能参考文献：
    1. The expression of M3-mAChR was down-regulated in the myocardium from aged mice and D-galactose-treated mice, while the expression levels of caspase-1 and its downstream molecule IL-1beta were significantly increased. PMID: 30196290
    2. Stimulation of the ERK pathway in Gnb5(-/-) cells by epidermal growth factor restored M3R-stimulated insulin release to near normal levels. Identification of the novel role of Gbeta5-R7 in insulin secretion may lead to a new therapeutic approach for improving pancreatic beta-cell function PMID: 28687610
    3. s. Moreover, we found that M3 cholinergic receptor (CHRM3) was upregulated in a large subset of benign prostatic hyperplasia (BPH) tissues compared with normal tissues. Activation of CHRM3 also promoted the proliferation of BPH cells. PMID: 27167157
    4. early intervention with cholinergic receptor muscarinic (ChRM)-3 blocker reversed the progression of airway hyperreactivity in the neonatal exposure model, whereas beta2-adrenoceptor agonists had no such effect PMID: 28619712
    5. We found that knockout of the M2/3 receptor significantly inhibited ST37 acupuncture-induced enhancement of gastric motility, jejunal motility, and colonic motility. PMID: 27978539
    6. Type 3 muscarinic receptors contribute to intestinal mucosal homeostasis and clearance of Nippostrongylus brasiliensis through induction of TH2 cytokines. PMID: 27173511
    7. Arthritis-induced joint destruction was significantly stronger in mice with M3 muscarinic acetylcholine receptor deficiency. PMID: 26785775
    8. The effects of muscarinic receptor M3 knockout on cathepsin K expression, bone density and biomechanical properties of bone are reported. PMID: 26002583
    9. results suggest that the autoantibodies against peptides of the second extracellular loop of M3R are not pathogenic in vivo and they are not suitable as biomarkers for pSS diagnosis PMID: 26901532
    10. Expression of the M3 receptors mediating cholinergic contractile stimuli of the detrusor muscle was dysregulated in both Mras-/- males and females, although only males exhibited a urinary phenotype. PMID: 26516777
    11. The study screened CrkL binding proteins using RNA interference (RNAi) and identified Sorbs1 and Sorbs2 as two proteins that are enriched at AChR clusters and are required for the formation of AChR aggregation in vitro. PMID: 26527617
    12. Activation of Chrm3 inhibits the recruitment of beta-arrestin-2 to odorant receptors, resulting in a potentiation of odor-induced responses in olfactory sensory neurons. PMID: 25800153
    13. The muscarinic M3R receptor interacts directly with NOSTRIN at the plasma membrane in the aorta. PMID: 26169369
    14. Cholinergic signalling via the M3R is essential for optimal Th1 and Th2 adaptive immunity to infection. PMID: 25629518
    15. These findings indicate that the M(3) receptor on structural cells plays a proinflammatory role in CS-induced neutrophilic inflammation, whereas the M(3) receptor on inflammatory cells does not. PMID: 25381025
    16. Our results identify a novel candidate mouse gene, Zfp277, whose expression pattern is compatible with a role in mediating divergent effects of Chrm3 and Chrm1 gene ablation on murine intestinal neoplasia. PMID: 24694019
    17. M3 acetylcholine receptors are increased in beta cells as a mechanism to compensate for amino acid deficiency PMID: 24695728
    18. In this review, genetic modifications of muscarinic M3 receptor cause robust alterations in insulin levels and glucose tolerance. PMID: 24114586
    19. The upregulation of M-mAChR during myocardial hypertrophy could relieve the hypertrophic response provoked by angiotensin II. PMID: 24028210
    20. Data indicate that acetylcholine contributes to allergen-induced remodeling and smooth muscle mass via the muscarinic M3 receptor. PMID: 24156289
    21. Activation of alpha2A-AR and muscarinic M3 receptors affects the initial [Ca(2+)] response to increase of glucose from 3 to 20mM in BETA-cells. PMID: 24565843
    22. M2 receptors play an essential role in the generation of intestinal rhythmic motor activity, and M3 receptors have a modulatory role in controlling the periodicity of the rhythmic activity PMID: 23889852
    23. These results suggest that mAChRs in mouse colonic epithelial cells consist of two subtypes, M1 (80 %) and M3 (20 %). PMID: 23242454
    24. M3 receptor and VGLUT2 are co-localized on intraganglionic laminar endings of esophagus. PMID: 23742744
    25. in murine ophthalmic arteries the muscarinic M3 receptor subtype mediates cholinergic endothelium-dependent vasodilation and endothelium-independent vasoconstriction. PMID: 24408978
    26. Oscillatory Ca2+ increase in response to M3 muscarinic acetylcholine receptor stimulation is dependent upon a moderate IP3 increase, which is suitable for causing Ca(2+)-dependent IP3-induced Ca2+ release. PMID: 23747049
    27. the restricted M3 muscarinic receptors expression contributes to the propagation Ca2+ signaling triggered by Acetylcholine. PMID: 23407022
    28. Immunization of muscarinic acetylcholine 3 receptor induces the secretion of IL-17 and IFN-gamma in NOD-scid mice. PMID: 23232510
    29. Blockage of mAChR exerts anti-inflammatory properties, and M3R plays an important role in the LPS-induced lung inflammation PMID: 22910223
    30. Data suggest that although M3R is located on both myocardial cells and endocardial endothelial cells, only endothelial M3R mediates positive inotropy in response to muscarinic agonists via activation of cyclooxygenase 2 in the atrium. PMID: 22726658
    31. These results indicate that alpha7-nAChR are not involved in the regulation of bone collagen synthesis whereas M3R exert stimulatory effects on cancellous bone microarchitecture, flexural rigidity, and bone matrix synthesis. PMID: 22871384
    32. Studies with M3R mutant mice strongly suggest that strategies aimed at enhancing signaling through beta-cell M3R receptor may be useful in the treatment of type 2 diabetes by promoting insulin release and improving beta-cell function in general.[review] PMID: 22525375
    33. Findings support the concept that the inhibitory effects of SPL on M3R activity are mediated by RGS4. PMID: 22730439
    34. M-mAChR overexpression dramatically reduced the incidence of arrhythmias and decreased the mortality in a mouse model of myocardial ischemia-reperfusion (I/R). PMID: 21785809
    35. interplay of Chrm3 and beta-catenin signaling is important for intestinal mucosal differentiation and neoplasia. PMID: 21705482
    36. Caveolae disruption decreased muscarine-induced bronchoconstriction in wild-type and abolished it in M2R(-/-) and M3R(-/-) mice. Thus M2R and M3R signaling pathways require intact caveolae. PMID: 20023174
    37. M receptors mediate cholinergic vasodilation in cutaneous, skeletal muscle, and renal interlobar arteries. PMID: 21335473
    38. The expression of M3R in different cell types during skin wound healing is time-dependent. PMID: 20707271
    39. M(3)-muscarinic receptor-mediated augmentation of sustained insulin release is largely independent of G protein-coupling but involves phosphorylation-/arrestin-dependent coupling of the receptor to protein kinase D1 PMID: 21078968
    40. Results suggest acetylcholine-M3 muscarinic receptor may be involved in itching associated with some chronic pruritus diseases such as atopic dermatitis. PMID: 20595784
    41. NF-kappaB signalling plays an essential role in controlling the expression of the muscarinic M(3) receptor. PMID: 20541544
    42. determination of m2 and m3 muscarinic receptors in the central auditory system PMID: 20665816
    43. Signaling through the M(3) muscarinic receptor favors bone mass accrual by decreasing sympathetic activity. PMID: 20197056
    44. The M3-muscarinic receptor regulates learning and memory in a receptor phosphorylation/arrestin-dependent manner. PMID: 20439723
    45. Chrm3 plays a critical role in the liver injury response by modulating hepatocyte proliferation and apoptosis PMID: 20197374
    46. M3 mAChRs in distinguish parvalbumin-positive from cholecystokinin-positive basket cells; findings demonstrate that cell type-specific cholinergic specializations are present on neurochemically distinct interneuron subtypes in the hippocampus PMID: 20427660
    47. RGS2 is an important cholinergic regulator in the atrium. RGS2(-/-) mice have enhanced susceptibility to atrial fibrillation via enhanced M3 muscarinic receptor activity. PMID: 19966055
    48. The role of M3 muscarinic receptor in smooth muscle contraction is analyzed in M3 knockout mice. PMID: 12486155
    49. When the muscarinic M3 receptor is deleted, cholinergic-induced relaxation is unmasked in the stomach fundus. PMID: 12538821
    50. The M3 mAChRs are involved in exocrine gland secretion, smooth muscle contractility, pupil dilation, food intake, and weight gain. Review. PMID: 12675128

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM3 sub-subfamily
  • 组织特异性：
    Expressed in cerebral cortex, submandibular gland, hypothalamus, pancreas, liver, and ileum.
  • 数据库链接：

    KEGG: mmu:12671

    STRING: 10090.ENSMUSP00000055579

    UniGene: Mm.342315