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  4. Recombinant Mouse Muscarinic acetylcholine receptor M4 (Chrm4)

Recombinant Mouse Muscarinic acetylcholine receptor M4 (Chrm4)

  • 货号：
    CSB-CF005384MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Chrm4; Chrm-4; Muscarinic acetylcholine receptor M4; Mm4 mAChR
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-479
  • 氨基酸序列
    MANFTPVNGSSANQSVRLVTTAHNHLETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNR QLQTVNNYFLFSLACADLIIGAFSMNLYTLYIIKGYWPLGAVVCDLWLALDYVVSNASVM NLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVP DNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKT LAFLKSPLMKPSIKKPPPGGASREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSA TQNTKERPPTELSTTEAATTPALPAPTLQPRTLNPASKWSKIQIVTKQTGSECVTAIEIV PATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMV LVNTFCQSCIPERVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
  • 基因功能参考文献：
    1. The findings of this study indicated that the cocaine-like S(D) effects of muscarinic antagonists are primarily mediated through M1 receptors, with a minor contribution of M4 receptors. PMID: 28442355
    2. In the present study, the functional coupling of Gi/o protein-coupled receptors to GalR1, and the CB1 receptor subtype for endocannabinoids were analyzed in the 3xTg-AD mice model of Alzheimer's disease in the prodromal and advanced stages. In addition, the activity mediated by Gi/o protein-coupled M2/4 muscarinic receptor subtypes was also analyzed in brain areas involved in anxiety and cognition. PMID: 27223629
    3. Muscarinic acetylcholine receptor subtype 4 is essential for cholinergic stimulation of duodenal bicarbonate secretion in mice - relationship to D cell/somatostatin PMID: 26084221
    4. Allosteric activation of M4 muscarinic receptors improve behavioral and physiological alterations in early symptomatic Huntington's disease - YAC128 mice. PMID: 26508634
    5. the role of the M4 receptor in alcohol consumption PMID: 25445043
    6. We show that M4 is a major mAChR subtype mediating the cholinergic inhibition of corticostriatal glutamatergic input on both striatonigral and striatopallidal medium spiny neurons (MSNs). PMID: 24528004
    7. The characterization of the orthosteric agonist, iperoxo, at the M(4) receptor. PMID: 24807965
    8. These data support a role for the M4AChR subtype in mediating the antipsychotic-like activity of BuTAC. PMID: 23907402
    9. Presence of M4 mAChR is sufficient and necessary to mediate the carbachol-induced membrane hyperpolarization and inhibition of SpECTs in laterodorsal tegmental nucleus neurons. PMID: 22956788
    10. M4R is involved in regulation of locomotor activity, social behavior, and sensorimotor gating in mice. PMID: 22463818
    11. Reducing M4 receptor signaling altered only select behavioral phenotypes in the Fmr1KO mouse model, suggesting that other targets are involved in the modulation of fragile X behaviors. PMID: 22123412
    12. Chrm4 receptor is located on presynaptic glutamatergic terminals afferent to the recorded pyramidal neuron, thus decreasing glutamate release. PMID: 20600670
    13. The muscarinic M(4) receptor is the functionally predominant subtype in mouse striatum. PMID: 21114972
    14. Different coupling of striatal M(2)/M(4)-mAChRs to the control of DA release in CPu versus NAc suggests targets to influence DA/ACh function differentially between striatal domains. PMID: 20203199
    15. a distinct subpopulation of neuronal M(4) mAChRs plays a critical role in modulating several important dopamine-dependent behaviors. PMID: 20147565
    16. muscarinic analgesia is exclusively mediated by a combination of M(2) and M(4) mAChRs at both spinal and supraspinal sites. PMID: 12391271
    17. M(4) mAChR plays a central role in mediating cholinergic control of keratinocyte migration by endogenous acetylcholine produced by these cells PMID: 12628458
    18. M4 mAChRs modulate dopamine activity in motor tracts and act as inhibitory autoreceptors in striatum. Review. PMID: 12675128
    19. There is a crucial role for M4 receptors in the tonic and phasic regulation of acetylcholine efflux in the hippocampus as well as in cognitive processes. The M4 receptor participates in a tonic regulatory loop contolling basal acetylcholine levels. PMID: 12874603
    20. loss of M4 muscarinic receptor control of cholinergic function effectuates a state of dopaminergic hyperexcitability PMID: 15231726
    21. M4 increases expression of "migratory" integrins; M4 effects resulted from inhibition of the inhibitory pathway involving the adenylyl cyclase-cyclic AMP-protein kinase A pathway PMID: 15263021
    22. This study indicate that both M4 mediate the muscarinic autoinhibition in ACh release in the longitudinal muscle with myenteric plexus preparation of the mouse ileum. PMID: 15574798
    23. M2 and M5 muscarinic receptors but not M4 muscarinic receptor or D2 dopamine receptors are needed for male USV production during male-female interactions PMID: 18382674
    24. Results suggest that GRK5 deficiency leads to a reduced hippocampal ACh release and cholinergic hypofunction by selective impairment of desensitization of presynaptic M2/M4 autoreceptors. PMID: 19478075

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM4 sub-subfamily
  • 数据库链接：

    KEGG: mmu:12672

    STRING: 10090.ENSMUSP00000040808

    UniGene: Mm.330405