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  4. Recombinant Mouse Neuronal acetylcholine receptor subunit beta-2 (Chrnb2)

Recombinant Mouse Neuronal acetylcholine receptor subunit beta-2 (Chrnb2)

  • 中文名称：
    Recombinant Mouse Neuronal acetylcholine receptor subunit beta-2(Chrnb2)
  • 货号：
    CSB-CF872133MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Chrnb2
  • Uniprot No.：
  • 别名：
    Chrnb2; Neuronal acetylcholine receptor subunit beta-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-501
  • 氨基酸序列
    TDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQ EWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFW LPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSDVASLDDFTPSGEWDI IALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEK MTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRS PTTHTMAPWVKVVFLEKLPTLLFLQQPRHRCARQRLRLRRRQREREGAGTLFFRKGPAAD PCTCFVNPASMQGLAGAFQAEPAAAGLGRSMGPCSCGLREAVDGVRFIADHMRSEDDDQS VREDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLFQNYTATTFLHSDHSAPSSK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions.
  • 基因功能参考文献：
    1. Data suggest that receptor complex assembly from Chrna4 and Chrnb2 on neuronal cell surface is up-regulated by nicotine; combined effects of increased assembly stoichiometry and preferential trafficking likely drive up-regulation of this receptor complex on neuronal cell surface upon nicotine exposure. (Chrna4 = a4 nicotinic receptor; Chrnb2 = b2 nicotinic receptor) PMID: 29074617
    2. study provides evidence that alpha6beta2 nAChRs are involved in locomotor activity as well as ethanol and saccharin consumption in adolescent animals PMID: 28457669
    3. Results suggest that alpha4beta2( *) nAChRs can produce sustained regulation of inhibitory post-synaptic GABA currents, in fast-spiking layer V neurons of the mouse frontal area 2. PMID: 27793780
    4. beta2 containing nicotinic acetylcholine receptors play roles in the memory deficits associated with dentate-gyrys specific amyloid beta expression. PMID: 27522251
    5. our results show that the beta2V287L ADNFLE mutation increases the ACh sensitivities of both HS and LS alpha4beta2 nAChRs (but not that of alpha5alpha4beta2 nAChRs). PMID: 27336596
    6. Varenicline treatment may prevent scopolamine-induced memory disturbance by a molecular mechanism that may involve increased alpha4beta2 nAChR availability. Varenicline dose-dependently increased protein expression of both the alpha4 and beta2 subunit in cell cultures and brain tissues, respectively, but had no effect on mRNA expression; lack of change in mRNA expression suggests a still unknown post-transcriptional me... PMID: 27012889
    7. MAFA controls autonomic nervous system-mediated insulin secretion by activating the transcription of nicotinic (ChrnB2 and ChrnB4) receptor genes, which is impaired in type 2 diabetes. PMID: 26904947
    8. Results demonstrate that high-affinity alpha4beta2 nicotinic acetylcholine receptors are necessary for the effects of acute nicotine on contextual fear extinction. PMID: 26688111
    9. Chrna7-deficient mice exhibit sustained attention impairments that are reversed by Chrnb2 activation PMID: 26222090
    10. results reveal an unanticipated role for subcortical nAChRs in motivation induced by expected uncertainty. PMID: 26780509
    11. Phenotype of layer V pyramidal cellss in aged Chrnb2 knockout mice is the result of the interaction of aging with the absence of high-affinity nAChRs over a relatively prolonged period of life span PMID: 24554727
    12. results showed that precipitated and spontaneous nicotine withdrawal-induced anhedonia was attenuated in beta2 knock-out mice. PMID: 25107281
    13. Lynx2 and Ly6g6e have roles in intracellular trafficking and allosteric potentiation of alpha4beta2 nAChRs, respectively PMID: 26276394
    14. cotinine alters the assembly and trafficking of alpha4beta2 nicotinic acetylcholine receptors PMID: 26269589
    15. The study shows that nAChR alpha4 beta2 sensitizes a MAPK-linked toxicity pathway on prolonged exposure to ABETA (1-42). PMID: 26139609
    16. This experiments establish a firm causal link between Chrnb2 retinal waves, and visual circuit development and demonstrate that spontaneous retinal waves are necessary and instructive for circuit refinement in the developing nervous system PMID: 25466916
    17. Lynx1 has a role in shifting alpha4beta2 nicotinic receptor subunit stoichiometry by affecting assembly in the endoplasmic reticulum PMID: 25193667
    18. Data suggest that the beta2 subunit of the nAChR is involved in the nicotine-induced inhibition of resident macrophages in stomach. PMID: 24223920
    19. This study demonistrated that Genetic deficiency of beta2-containing nicotinic receptors attenuates brain injury in ischemic stroke in mice. PMID: 24184117
    20. anatomical and developmental evidence suggests that the nAChR alpha7 and beta2 subunits may involve in the early direction-selectivity formation of DS RGCs in the mouse retina. PMID: 24631336
    21. Cerebral cortex beta2 nAChR subunits increased in rats given nicotine or varenicline, but subunits from rats given saline or saz-A during the phase 2 treatment were not significantly different from all-phase saline controls. PMID: 24422997
    22. By stimulating alpha4beta2-nicotinic acetylcholine receptors in the prefrontal cortex, working memory deficits are alleviated in aged mice. PMID: 23352115
    23. Inhibition of alpha7 nicotinic acetylcholine receptors protects the function of acute hippocampal slices against excitotoxicity in an alpha4beta2-dependent manner. PMID: 23280428
    24. Our findings suggest nAChR beta2 as a prominent pathway for nicotine induced impairment of NK cell functions which contributes to the occurrence of smoking-related pathologies PMID: 23469004
    25. prospectively carried out a high-resolution 3-T magnetic resonance imaging (MRI) study in an ADNFLE family containing 10 affected members including one pharmacoresistant patient and carrying the V287L mutation of the CHRN beta2 subunit PMID: 22897520
    26. post-receptor calcium-dependent mechanisms associated with nicotine withdrawal are mediated through beta2-containing nicotinic receptors PMID: 23313759
    27. beta2*- and alpha9*-nAChRs, in addition to alpha7-nAChRs, have different roles in endogenous and nicotine-dependent modulation of immune functions and could be exploited as therapeutic targets to modulate inflammation and autoimmunity. PMID: 23399696
    28. Data indicate that functional alpha4/beta2 nicotinic acetylcholine receptors (alpha4beta2 nAChR) plays a role in proliferation and differentiation fate into a neuronal lineage in neural progenitors of developing neocortex. PMID: 23056257
    29. mice which lack nAChRs containing the beta2 subunit (beta2* nAChRs) exhibit compromised auditory discrimination, suggesting that beta2* nAChRs are necessary for normal auditory discrimination or that beta2* nAChRs play a critical role in auditory pathway development. PMID: 22433585
    30. beta2-nAChRs are essential for acquisition of normal numbers of dendritic spines during development. PMID: 22699919
    31. Varenicline acts as a functional antagonist of Chrnb2, blocking certain effects of nicotine. PMID: 22241831
    32. Our results support the view that beta2*nAChRs modulate the prefrontal cortex (PFC) activity, specifically in flexible choice making PMID: 21810785
    33. beta2-nicotinic acetylcholine receptors are implicated in the recruitment of inhibitory control of nociception, as shown by delayed recovery from capsaicin-induced allodynia. PMID: 21794985
    34. find that Trask expression is retained, reduced or sometimes lost in some tumors compared with their normal epithelial tissue counterparts PMID: 21706059
    35. structural changes indicative of accelerated aging are evident in young beta2 -/- mice, the data suggest however, that nAChRs do not directly regulate expression of survival genes. PMID: 21939740
    36. A novel mutant mouse harboring the human ADNFLE mutation in the Chrnb2 gene was generated and used to study the roles of beta2* nicotinic receptors in complex biological processes including the activity-rest cycle, natural reward and anxiety. PMID: 20603624
    37. The data of this study provided evidence for an important role of beta2*nAChRs in tasks involving motivation ranking. PMID: 21784105
    38. These findings suggested that disrupting nicotinic receptors fundamentally alters the mechanisms and timing of excitation in prefrontal attentional circuitry. PMID: 22072695
    39. chronic nicotine substantially increased the number of alpha4beta2-containing vesicle fusions at the plasma membrane; this stage in alpha4beta2 nAChR up-regulation is presumably downstream from increased endoplasmic reticulum exit PMID: 21768117
    40. Protection from acoustic trauma in older Chrnb2 knockout mice is mainly mediated by an age-related increase of corticosterone. PMID: 21272629
    41. That dopaminergic Chrnb2 are involved in nicotine CPP, anxiety reduction, and locomotor suppression. PMID: 21795541
    42. Data SHOW that social interactions particularly mobilize the PrL area of the mouse PFC and that the presence of functional PrL beta2*-nAChRs is necessary for the integrated behavior to emerge. PMID: 21402717
    43. The retinas of Chrnb2-/- mutants are normal in appearance, but the increased expression of these genes may also be involved in the abnormal projection patterns of RGC to the LGN. PMID: 21547082
    44. findings show expression of beta2*-nAChRs is necessary for optimal attentional performance and that restoring expression of beta2*-nAChRs in the medial prefrontal cortex prelimbic area is sufficient for optimal performance PMID: 21836018
    45. In the acute MPTP model of Parkinson's disease, time-courses of altered expression for nAChR alpha7, alpha4 and beta2 subunits closely mirrored the deficits observed in animal motor activity PMID: 21406211
    46. Expression of beta2-nicotinic acetylcholine receptors only in retinal ganglion cells shows that not merely the presence, but the precise spatiotemporal pattern of spontaneous retinal activity instructs neural circuit development. PMID: 21689598
    47. propose that in the P3 superior cervical ganglion of wild type mice, alpha3beta4 (+/-alpha5) represent about 62% of receptors, whereas 17% are alpha3beta2beta4, and 21% are alpha4beta2 (+/-alpha5) receptors PMID: 21485013
    48. the beta2 nicotinic receptor subunit is involved in the dendritic morphogenesis of pyramidal neurons and in the circuits that contribute to the high-order functional connectivity of the cerebral cortex PMID: 20534523
    49. Prefrontal alpha4beta2* nAChRs play a key role in evoking and facilitating the transient glutamatergic-cholinergic interactions that are necessary for cue detection and attentional performance. PMID: 20203212
    50. Results show thatlynx1 promotes the largest of three current amplitudes elicited by ACh through alpha(4)beta(2) nAChRs and that lynx1 enhances receptor desensitization. PMID: 11906696

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Beta-2/CHRNB2 sub-subfamily
  • 数据库链接：

    KEGG: mmu:11444

    STRING: 10090.ENSMUSP00000029562

    UniGene: Mm.35088