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  4. Recombinant Mouse Potassium channel subfamily K member 1 (Kcnk1)

Recombinant Mouse Potassium channel subfamily K member 1 (Kcnk1)

  • 中文名称：
    小鼠Kcnk1重组蛋白
  • 货号：
    CSB-CF012062MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Kcnk1
  • Uniprot No.：
  • 别名：
    Kcnk1; Potassium channel subfamily K member 1; Inward rectifying potassium channel protein TWIK-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-336
  • 氨基酸序列
    MLQSLAGSSCVRLVERHRSAWCFGFLVLGYLLYLVFGAVVFSSVELPYEDLLRQELRKLK RRFLEEHECLSEPQLEQFLGRVLEASNYGVSVLSNASGNWNWDFTSALFFASTVLSTTGY GHTVPLSDGGKAFCIIYSVIGIPFTLLFLTAVVQRVTVHVTRRPVLYFHIRWGFSKQVVA IVHAVLLGFVTVSCFFFIPAAVFSVLEDDWNFLESFYFCFISLSTIGLGDYVPGEGYNQK FRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKMFYVKKDKDEDLVHIMEHDQLS FSSVTEQVAGLKEEQKQSEPFVASQSPPYEDGSADH
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ion channel that contributes to passive transmembrane potassium transport and to the regulation of the resting membrane potential in brain astrocytes, but also in kidney and in other tissues. Forms dimeric channels through which potassium ions pass in accordance with their electrochemical gradient. The channel is selective for K(+) ions at physiological potassium concentrations and at neutral pH, but becomes permeable to Na(+) at subphysiological K(+) levels and upon acidification of the extracellular medium. The homodimer has very low potassium channel activity, when expressed in heterologous systems, and can function as weakly inward rectifying potassium channel. Channel activity is modulated by activation of serotonin receptors. Heterodimeric channels containing KCNK1 and KCNK2 have much higher activity, and may represent the predominant form in astrocytes. Heterodimeric channels containing KCNK1 and KCNK3 or KCNK9 have much higher activity. Heterodimeric channels formed by KCNK1 and KCNK9 may contribute to halothane-sensitive currents. Mediates outward rectifying potassium currents in dentate gyrus granule cells and contributes to the regulation of their resting membrane potential. Contributes to the regulation of action potential firing in dentate gyrus granule cells and down-regulates their intrinsic excitability. In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1. Required for normal ion and water transport in the kidney. Contributes to the regulation of the resting membrane potential of pancreatic beta cells. The low channel activity of homodimeric KCNK1 may be due to sumoylation. The low channel activity may be due to rapid internalization from the cell membrane and retention in recycling endosomes.
  • 基因功能参考文献：
    1. the activation of mGluR3 up-regulates the membrane expression of TWIK-1 that in turn enhances NH4(+) uptake in astrocytes. PMID: 26553349
    2. KCNK1 was significantly induced during osteoclast differentiation, but its functional overexpression significantly inhibited osteoclast differentiation induced by RANKL PMID: 26208638
    3. TWIK-1 is functionally expressed in dentate gyrus granule cells and contributes to the intrinsic excitability of these cells. PMID: 25406588
    4. TWIK-1/TREK-1 heterodimers mediate astrocytic passive conductance and cannabinoid-induced glutamate release from astrocytes. PMID: 24496152
    5. ion selectivity of TWIK-1 K+ channels during pathological hypokalemia; a molecular basis for inward leak Na+ currents that could trigger or contribute to cardiac paradoxical depolarization in lowered [K+]o; mechanism for regulating cardiac excitability. PMID: 21653227
    6. Specifically localized in 'non-sensory' cells of inner ear, in dark cells of vestibule and in strial marginal cells of cochlea. May have role in regulation of K(+) homeostasis in dark cells and in strial marginal cells. PMID: 12855359
    7. A fall in quinidine-sensitive conductance could be an adaptive response in TWIK-1 knockout ducts. PMID: 16847696

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Recycling endosome. Apical cell membrane. Cytoplasmic vesicle. Perikaryon. Cell projection, dendrite. Cell projection. Cell junction, synapse, synaptic cell membrane.
  • 蛋白家族：
    Two pore domain potassium channel (TC 1.A.1.8) family
  • 组织特异性：
    Detected in spiral ganglion neurons. Detected in hippocampus CA1 and CA1 regions and in the molecular layer of the dentate gyrus. Detected on hippocampus astrocytes. Highly expressed in the stria vascularis in the cochlea. Detected in pancreas islet beta
  • 数据库链接：

    KEGG: mmu:16525

    STRING: 10090.ENSMUSP00000046103

    UniGene: Mm.10800