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  4. Recombinant Mouse Potassium channel subfamily K member 2 (Kcnk2)

Recombinant Mouse Potassium channel subfamily K member 2 (Kcnk2)

  • 中文名称：
    小鼠Kcnk2重组蛋白
  • 货号：
    CSB-CF012070MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Kcnk2
  • Uniprot No.：
  • 别名：
    Kcnk2; Potassium channel subfamily K member 2; Outward rectifying potassium channel protein TREK-1; TREK-1 K(+ channel subunit; Two pore potassium channel TPKC1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-411
  • 氨基酸序列
    MAAPDLLDPKSAAQNSKPRLSFSSKPTVLASRVESDSAINVMKWKTVSTIFLVVVLYLII GAAVFKALEQPQEISQRTTIVIQKQTFIAQHACVNSTELDELIQQIVAAINAGIIPLGNS SNQVSHWDLGSSFFFAGTVITTIGFGNISPRTEGGKIFCIIYALLGIPLFGFLLAGVGDQ LGTIFGKGIAKVEDTFIKWNVSQTKIRIISTIIFILFGCVLFVALPAVIFKHIEGWSALD AIYFVVITLTTIGFGDYVAGGSDIEYLDFYKPVVWFWILVGLAYFAAVLSMIGDWLRVIS KKTKEEVGEFRAHAAEWTANVTAEFKETRRRLSVEIYDKFQRATSVKRKLSAELAGNHNQ ELTPCRRTLSVNHLTSEREVLPPLLKAESIYLNGLTPHCAGEDIAVIENMK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner. In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2. In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1.
  • 基因功能参考文献：
    1. Data demonstrate that inhibition of TWIK-related K(+) channel-1 (TREK-1) protects mice from cognitive impairment induced by anesthesia and TREK-1 is a potential therapeutic target against memory impairment induced by volatile anesthetics. PMID: 29042297
    2. data reveal a druggable K2P site that stabilizes the C-type gate 'leak mode' and provide direct evidence for K2P selectivity filter gating PMID: 28693035
    3. Hyperoxia treatment of TREK-1/TREK-2/TRAAK-deficient mice is associated with a reduction in surfactant proteins PMID: 28839101
    4. TREK-1 deficiency promoted neurons and oligodendrocytes apoptosis, aggravated demyelination and retarded motor recovery following spinal cord injury. PMID: 28192611
    5. essential for normal sinoatrial node cell excitability PMID: 27098968
    6. Formation of TREK-1/TREK-2 channels was also demonstrated in native dorsal root ganglion neurons indicating that heterodimerization may provide greater diversity of leak K(+) conductances also in native tissues. PMID: 27129242
    7. Trek1 expression facilitates the restoration of intestinal epithelial barrier functions in an allergic environment. PMID: 25683610
    8. Data suggest that porosome-associated proteins SNAP25, TREK-1, syntaxin-1A, and Gai3 exhibit stability and functionality such that isolated proteins can be reconstituted as insulin-secreting porosomes in cell membrane of live cells. PMID: 26523491
    9. Data indicate that arginine vasopressin (AVP) and corticotropin-releasing hormone (CRH) show additive effects on the suppression of the potassium channel subfamily K member 2 TREK-1 current via protein kinase C. PMID: 26248219
    10. In TREK1-deficient mice, brain endothelial cells displayed an inflammatory phenotype. PMID: 24557892
    11. provide new insight into membrane targeting of TREK-1 in the heart and establish a broader role for beta(IV)-spectrin in organizing functional membrane domains critical for normal heart function PMID: 24445605
    12. TREK-1 channels significantly contribute to the responsiveness of Grueneberg ganglion neurons to cool temperatures. PMID: 24101433
    13. direct activation of the TREK-1 K(+) channel, acting downstream from the mu opioid receptor, might have strong analgesic effects without opioid-like adverse effects PMID: 24346231
    14. TREK-M inhibits neuronal firing by hyperpolarizing the resting membrane potential and decreasing input resistance. Delivery of TREK-M could reduce the duration of status epilepticus and reduce neuronal death. PMID: 24299204
    15. TREK1 knockout mice exhibit altered retinal waves. PMID: 23390312
    16. TREK-1 is directly sensitive to membrane tension. PMID: 23897808
    17. study analyzed the role of TWIK-related potassium channel-1 (TREK1)in endothelial cells and the blood-brain barrier(BBB); blocking TREK1 increased leukocyte transmigration; TREK1 activation had the opposite effect; results suggest an ion channel expressed by endothelial cells at the BBB regulates immune cell trafficking into the inflamed CNS PMID: 23933981
    18. Polycystins protect renal epithelial cells against apoptosis in response to mechanical stress, and this function is mediated through the opening of stretch-activated K(2P) channels. PMID: 22832196
    19. Trek-1 deficiency decreased IL-6 secretion from mouse and human alveolar epithelial cells at both transcriptional and translational levels PMID: 23275623
    20. Ultrastructural analyses demonstrate that TREK-1 is preferentially localized at cell body and processes, whereas Best1 is mostly found in microdomains of astrocytes near synapses. PMID: 23021213
    21. This review presented that the TERK-1 knockout mice show a correlation between 5-HT firing rate and PMID: 23089640
    22. A mechanism for signal transduction within K(2P)2.1 (TREK-1) in which there is a clear crosstalk between the C-type gate and intracellular Ct domain. PMID: 22728824
    23. Mice that did not express TREK-1 had lower decompression sickness resistance and were more likely to develop neurological symptoms PMID: 22323654
    24. TREK-1 was expressed and could be detected on smooth muscle cells surrounding intrapulmonary airways and blood vessels. PMID: 21822716
    25. Popdc1 and Popdc2 proteins interact with the potassium channel TREK-1, which leads to increased cell surface expression and enhanced current density PMID: 22354168
    26. Expression of Trek-1 was significantly downregulated in cultured cells and lungs of mice exposed to hyperoxia.In MLE-12 cells Trek-1 defficiency resulted in increased cell proliferation and upregulation of PCNA and decrease of IL-6 and RANTES secretion. PMID: 21949155
    27. TREK-1-expressing cells are more prevalent in lumbosacral than thoracolumbar ganglia in both bladder- and colon-dorsal root ganglia neurons. PMID: 21549810
    28. Our results define a unique gating mechanism shared by K2P family members such as TREK-1 and suggest that their diverse sensory properties are achieved by coupling different molecular sensors to a conserved core gating apparatus. PMID: 21765396
    29. Our data suggest that uterine excitability and contractility during pregnancy is regulated by the expression of SDK/TREK-1 channels. PMID: 21224218
    30. Data suggest that the current is mediated via TWIK-related K(+) channel (TREK)-1 channels. PMID: 21343252
    31. Report thermodynamic analysis of mouse TREK-1 gating and functional testing of several deletion mutants and compare to structural models. PMID: 21057213
    32. TREK-1 expression does not provide brain protection after traumatic brain injury. PMID: 21157470
    33. inhibition of TREK1 current by fluoxetine is found to be accompanied by dissociation of the C-terminal domain from the membrane. PMID: 21262820
    34. Data identify a splice variant of TREK1 expressed in the brain, which encodes a heavily truncated TREK1 protein retaining a single transmembrane domain, having no channel activity itself but reduced TREK1 whole cell current amplitude. PMID: 20605797
    35. Conclude that regulation of arterial diameter is not altered in mice lacking TREK-1. PMID: 20357027
    36. Results describe the antidepressant effects of spadin, a secreted peptide derived from the propeptide generated by the maturation of the neurotensin receptor 3 (NTSR3/Sortilin) and acting through TREK-1 inhibition. PMID: 20405001
    37. TREK channels may represent the AMPK-inhibited background K(+) channels that mediate activation of glomus cells by hypoxia. PMID: 19840997
    38. protonation of the E306 residue tunes the TREK-1 mechanical setpoint and thus sets lipid sensitivity PMID: 12065410
    39. TREK-1 is involved in neuroprotection and general anesthesia. PMID: 15175651
    40. The TREK-1 channel protein was immunodetected in both nerve fibers and nerve cell bodies in the vestibular ganglion, both afferent fibers and nerve calyces innervating type I hair cells in the utricle and cristae. PMID: 15261098
    41. TREK-1, TREK-2, and TRAAK are lysophosphatidic acid-operated K+ channels PMID: 15572365
    42. Results demonstrate that membrane phospholipids, including PIP(2), control channel gating and transform TREK-1 into a leak potassium conductance. PMID: 15577940
    43. TREK-1 markedly alters the cytoskeletal network and induces the formation of actin- and ezrin-rich membrane protrusions. PMID: 15976821
    44. receptor- and kinase-induced inhibition of TREK-1 background potassium channels is mediated by sequential phosphorylation PMID: 16006563
    45. primary observation is that the mechanosensitive K2P channels TREK-1 and TRAAK desensitize; desensitization and its regulation by chemical messengers is predicted to condition the physiological role of K2P channels PMID: 16636285
    46. Results demonstrate that TREK-1 qualifies as one of the molecular sensors involved in pain perception. PMID: 16675954
    47. The association of AKAP150 with TREK channels integrates them into a postsynaptic scaffold where both G-protein-coupled membrane receptors and TREK-1 dock simultaneously. PMID: 17110924
    48. Deletion of TREK1 in mice leads to an important alteration in vasodilation of mesenteric arteries induced by acetylcholine and bradykinin. PMID: 17347672
    49. selective expression and activation of TREK-1 in brain collaterals could play a significant role in the protective mechanisms of polyunsaturated fatty acids against stroke by providing residual circulation during ischemia. PMID: 17556656
    50. the reduced sensitivity of glutamate and GABA release to inhibition by halothane in TREK-1 KO nerve terminals correlates with the reduced anaesthetic potency of halothane in TREK-1 KO mice PMID: 17828284

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Multi-pass membrane protein.; [Isoform 2]: Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Two pore domain potassium channel (TC 1.A.1.8) family
  • 组织特异性：
    Detected in hippocampus astrocytes (at protein level). High expression in brain and lung. Also detected in kidney, heart and skeletal muscle. Not detected in liver. In the brain, highest expression in olfactory bulb, hippocampus and cerebellum.
  • 数据库链接：

    KEGG: mmu:16526

    STRING: 10090.ENSMUSP00000078416

    UniGene: Mm.33304