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  4. Recombinant Mouse Prolyl endopeptidase FAP (Fap), partial

Recombinant Mouse Prolyl endopeptidase FAP (Fap), partial

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  • 中文名称：
    小鼠Fap重组蛋白
  • 货号：
    CSB-CF008424MO
  • 规格：
    ￥5028
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF008424MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Fap.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF008424MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Fap.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Fap
  • Uniprot No.：
  • 别名：
    Fap; Prolyl endopeptidase FAP; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha; FAPalpha; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Serine integral membrane protease; SIMP; Surface-expressed protease; Seprase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Extracellular Domain
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    101.3kDa
  • 表达区域：
    26-761aa
  • 氨基酸序列
    LRPSRVYKPEGNTKRALTLKDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    This recombinant MouseFap protein is an in vitro E.coli (cell-free) expressed Extracellular Domain protein. Its purity is 90%+ determined by SDS-PAGE. Cell-free protein expression is the in vitro synthesis of a protein using translation-compatible extracts of whole cells. In principle, whole-cell extracts contain all the macromolecules and components needed for transcription, translation, and even post-translational modification. These components include RNA polymerase, regulatory protein factors, transcription factors, ribosomes, and tRNA. When supplemented with cofactors, nucleotides, and the specific gene template, these extracts can synthesize proteins of interest in a few hours.

    Fap is a membrane-inserted prolyl-specific serine protease with both endopeptidase and dipeptidase activities. It is specifically expressed by active fibroblasts. Reactive stromal fibroblasts overexpress Fap during the growth of epithelial-derived tumors. Fap is upregulated in many types of human cancers, including breast, brain, and ovarian cancers. Mounting findings have revealed that Fap expression affects tumor growth by influencing tumor cell proliferation and invasion, angiogenesis, epithelial-to-mesenchymal transition (EMT), immunosuppression, and drug resistance.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein. Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
  • 基因功能参考文献：
    1. the uptake of the tracer in the joints reflects increased FAP expression during subclinical synovitis and that these joints will show symptoms of inflammation upon disease progression PMID: 29361119
    2. Adora2B stimulation promotes FGF2 and CXCL12 expression in FAP-positive melanoma-associated fibroblasts, contributing to the creation of a tumor-promoting microenvironment. PMID: 27590504
    3. There was no evidence of compensatory upregulation of other DPP4 family members in influenza-infected FAP-deficient mice. FAP appears to be dispensable in anti-influenza adaptive immunity. PMID: 28158223
    4. FAP-STAT3-CCL2 signaling in Cancer-associated fibroblasts (CAF) was sufficient to program an inflammatory component of the tumor microenvironment, which may have particular significance in desmoplasia-associated cancers. PMID: 27216177
    5. this study shows that FAPalpha-targeted modified vaccinia ankara boosting in combination with cyclophosphamide is an effective approach to improving specific anti-tumor immune responses through overcoming immunosuppression PMID: 27545090
    6. Taken together, our study suggested that high FAP expression in CAFs is one reason leading to immune checkpoint blockades resistance in CRC patients and FAP is an optional target for reversing immune checkpoint blockades resistance. PMID: 28302482
    7. FAP-vaccinated mice also treated with Cyclophosphamide chemotherapy showed a marked suppression of tumor growth (inhibition ratio =80%) and a prolongation of survival time. PMID: 28004985
    8. In two different models of pulmonary fibrosis, intratracheal bleomycin instillation and thoracic irradiation, the study finds increased mortality and increased lung fibrosis in FAP-deficient mice compared with wild-type mice. PMID: 26663085
    9. Mouse FGF-21, however, lacks the FAP cleavage site and is not cleaved by FAP. PMID: 26962859
    10. Data indicate that indolamine-2,3-dioxygenase (IDO) and Fibroblast activation protein alpha (FAPalpha) were detectable in B16 melanoma tumor-bearing mice. PMID: 26305550
    11. A transgenic mouse model for pulmonary fibrosis was generated. After bleomycin induction, luciferase cDNA under the control of the FAPa promoter presents strong luminescence in the lungs especially; the expression level reflects the degree of the disease. PMID: 25994578
    12. The FAP(+) stromal cell may have roles in two adverse consequences of cancer. PMID: 23712428
    13. In NIH 3T3 cells overexpressing recombinant mouse FAP, FAP enzymatic activity during matrix production is important for the topographical organization of the ECM fibers. PMID: 21668992
    14. s conclude that the proteolytic activity of FAP participates in matrix degradation, but other functions of the protein stimulate increased tumor growth. PMID: 21604185
    15. FAP may be a potentially useful marker for wound age determination. PMID: 20225612
    16. Results suggest that FAP, a product preferentially expressed by TAF, could function as an effective tumor rejection antigen. PMID: 20804499
    17. FAP depletion inhibits tumor cell proliferation indirectly, increases accumulation of collagen, decreases myofibroblast content, and decreases blood vessel density in tumors. PMID: 19920354
    18. the enzymatic activity of fibroblast activation protein plays an important role in the promotion of tumor growth PMID: 15767544
    19. seprase may have a role in promotion of an invasive phenotype by collagenous matrices in ovarian tumor cells PMID: 18823010

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  • 亚细胞定位：
    [Prolyl endopeptidase FAP]: Cell surface. Cell membrane; Single-pass type II membrane protein. Cell projection, lamellipodium membrane; Single-pass type II membrane protein. Cell projection, invadopodium membrane; Single-pass type II membrane protein. Cell projection, ruffle membrane; Single-pass type II membrane protein. Membrane; Single-pass type II membrane protein.; [Antiplasmin-cleaving enzyme FAP, soluble form]: Secreted.
  • 蛋白家族：
    Peptidase S9B family
  • 组织特异性：
    Expressed strongly in uterus, pancreas, submaxillary gland and skin, less in lymph node, ovary, skeletal muscle, adrenal and bone marrow. Expressed in reactive stromal fibroblast in epithelial cancers. Expressed in melanocytes but not melanomas (at protei
  • 数据库链接：

    KEGG: mmu:14089

    STRING: 10090.ENSMUSP00000099793

    UniGene: Mm.41816