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  4. Recombinant Mouse T-cell surface glycoprotein CD8 alpha chain (Cd8a)

Recombinant Mouse T-cell surface glycoprotein CD8 alpha chain (Cd8a)

  • 货号：
    CSB-CF004966MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Cd8a; Lyt-2; Lyt2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD antigen CD8a
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    28-247
  • 氨基酸序列
    KPQAPELRIFPKKMDAELGQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSAMRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSPVHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHRSRKRVCKCPRPLVRQEGKPRPSEKIV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
  • 基因功能参考文献：
    1. Depletion of Depletion of medullary thymic epithelial cells (mTECs) and CD8 antigen alpha chain (CD8alpha+) dendritic cells (DCs) leads to overt autoimmunity. PMID: 27543048
    2. Batf3 deficiency is not critical for the generation of CD8alpha dendritic cells PMID: 25468565
    3. Lck-CD8 interaction is required for initial CD8 recruitment. PMID: 25427562
    4. Brd1-mediated Hbo1 activity is crucial for efficient activation of CD8 expression via acetylation at H3K14, which serves as an epigenetic mark that promotes the recruitment of transcription machinery to the CD8 enhancers. PMID: 25519988
    5. Ly49E is expressed on a high proportion of CD8alphaalpha-positive intestinal intraepithelial lymphocytes. PMID: 25310588
    6. Comparing the sequences of mouse, human, rat and dog Cd8a and Cd8b1 gene loci identified 10 evolutionarily conserved regions (ECR). 6 ECRs overlapped with previously identified Cd8 enhancers. ECR-4 recruits transcription factors to the Cd8ab gene complex. PMID: 25548254
    7. Data indicate that orphan G-protein-coupled receptor GPR18 is required for the normal homeostasis of CD8alphaalpha gammadeltaT and alphabetaT and CD8alphabeta intestinal intraepithelial lymphocyte compartment. PMID: 25348153
    8. CD8alpha DCs, rather than CD8 T cells, are responsible for enhanced viral latency and recurrences. PMID: 24695322
    9. Data show that targeting nanoparticles containing alpha-galactosylceramide (alpha-GalCer) and Ag to CD8alpha(+) dendritic cells promotes potent antitumor responses, both in prophylactic and in therapeutic settings. PMID: 24913977
    10. Downregulation of CD8 during type 2 olarization of murine CD8(+) effector T cells is associated with CpG methylation of several regions of the Cd8a locus. PMID: 24675400
    11. Data indicate that CD8alpha knockout mice reconstituted with CD8(+) T cells restored the sensitivity to Ang II. PMID: 24600037
    12. CREMalpha orchestrates epigenetic remodeling of the CD8A,B through the recruitment of DNA methyltransferase (DNMT) 3a and histone methyltransferase G9a. PMID: 24297179
    13. Therefore, EXOs derived from natural CD4(+)25(+) and CD8(+)25(+) Tr cells may become an alternative for immunotherapy of autoimmune diseases. PMID: 23876314
    14. Data indicate that CD8low cells survive long term in vivo. PMID: 23311920
    15. A previously undescribed pathway by which CD8alpha+ Dendritic cells emerge independent of Id2, Nfil3, and Batf3, but dependent on Irf8. PMID: 23297132
    16. Age-associated alterations in CD8alpha+ dendritic cells impair CD8 T-cell expansion in response to an intracellular bacterium. PMID: 22862959
    17. During establishment of viral persistence, the cellular subset with the most prevalent expression of IL-10 was CD8alpha(-)CD4(+) dendritic cells (DCs), which produced IL-10 with increasing kinetics until 9 d postinfection. PMID: 22893686
    18. CD8 marks a population of lung airway-derived dendritic cells with distinct migratory and maturation responses that likely contribute differentially to the immune response depending on the infecting pathogen PMID: 22811544
    19. Thymic signal-regulatory protein (Sirp)alpha-positive conventional dendritic cells (cDCs) and CD8alpha-positive cDCs are equally competent in mediating presentation events to blood-borne hen egg white lysozyme by major histocompatibility class II molecules. PMID: 21178002
    20. In the small intestine of wild type mice, the majority of intraepithelial lymphocytes (IEL) harbor the CD8alphaalpha+ T cell phenotype, whereas conventional CD8alphabeta+ IEL account for ~20% of all IEL. PMID: 22753943
    21. Vitamin B6 supplementation downregulates Cd8a and Ccl8 mRNA expression, so these candidate genes may play a protective role against colonic aberrant crypt foci development. PMID: 22495988
    22. The presence of CD8-positive induced regulatory T (Treg) cells is sufficient to prevent increased graft-vs-host disease mortality in the complete absence of CD4-positive Tregs. PMID: 22649199
    23. Silencing Hsp25/Hsp27 gene expression augments proteasome activity and increases CD8+ T-cell-mediated tumor killing and memory responses PMID: 22185976
    24. The Cd8 coreceptor gene locus co-opts any coreceptor protein encoded within it to induce thymocytes to express the cytotoxic-lineage factor Runx3 and to adopt the cytotoxic-lineage fate. PMID: 22036949
    25. CD8alpha+ dendritic cells interact with monocytes in response to Listeria monocytogenes infection, which attenuates T cell responses PMID: 21559416
    26. Data demonstrated that CD207(+) CD8alpha(+) thymic DCs do not share a common origin with T cells but originate from intrathymic precursors. PMID: 21630253
    27. NFIL3 plays an essential role in the development of CD8alpha(+) cDCs. PMID: 21474667
    28. Data show a subset of CD103+CD8alpha+ LPDCs express TLR3, TLR7, and TLR9. PMID: 21525388
    29. Data show that the chromatin of the CD8 locus is remodeled in C57BL/6 and B6/J Rag1(-/-) MOM double-negative thymocytes. PMID: 21515796
    30. Flt3L-mobilized dendritic cells bearing H2-Kbm1 apoptotic cells do not induce cross-tolerance to CD8+ T cells across a class I MHC mismatched barrier PMID: 21276089
    31. Liposome-coupled antigens are internalized by antigen-presenting cells via pinocytosis and cross-presented to CD8 T cells PMID: 21179411
    32. In the absence of the vitamin D receptor, fewer CD8alphaalpha-expressing T cells develop in an increasingly inflamed gastrointestinal tract. PMID: 21270396
    33. Pre-CD8alpha-positive dendritic cells do not efficiently cross-present antigen from necrotic cells. Importantly, they acquire the ability to cross-present antigen to CD8 T cells if activated by Toll-like receptor ligands before antigen capture. PMID: 21187449
    34. Naturally occurring T regulatory cells from CD8alpha antigen-deficient mice fail to suppress lung allergic responses and are characterized by reduced levels of Foxp3, interleukin (IL)-10, and transforming growth factor-beta, and high levels of IL-6. PMID: 21115736
    35. CD8 gene expression in murine lymphocytes is accompanied by the relocation of the locus outside its subchromosomal territory; in nonexpressing cells, the gene remains close to the main body of its chromosomal domain. PMID: 20404270
    36. full thymocyte differentiation requires additional coreceptor activities conferred by CD8alphaalpha and/or CD8alphabeta PMID: 19950178
    37. When splenic dendritic cell (DC) populations are sorted from animals administered protein intravenously, the ability to cross-present antigen is restricted to the langerin-expressing subset of the CD8alpha-positive DC population. PMID: 19923446
    38. T cell responses modulated through interaction between CD8alphaalpha and the nonclassical MHC class I molecule, TL PMID: 11729321
    39. Soluble nonclassical thymic leukemia (TL) antigen has been shown to bind the homodimer CD8 alpha alpha with a 10 times higher affinity than the class I antigen H-2Kb. PMID: 11937542
    40. CD8(+) i-IEL play an important role in protection against 5-FU-induced lethality with translocation of Enterobacteriaceae PMID: 11986258
    41. Variegated expression of CD8 alpha resulting from in situ deletion of regulatory sequences PMID: 12049716
    42. Characterization of a new subpopulation of mouse CD8alpha+ B220+ dendritic cells endowed with type 1 interferon production capacity and tolerogenic potential. PMID: 12091326
    43. Identification of CD8alpha+CD11c- lineage phenotype-negative cells in the spleen as committed precursor of CD8alpha+ dendritic cells. PMID: 12091350
    44. dendritic cell expression of CD8alpha and IL-12 p40 require ICSBP expression PMID: 12393459
    45. This gene locus is regulated by the Ikaros family of proteins. PMID: 12504015
    46. Data demonstrate that major histocompatibility complex-I-signaled thymocytes appear as CD4+8lo cells because of transient down-regulation of CD8 gene expression, not because of changes in CD8 surface protein expression or distribution. PMID: 12810689
    47. CD8alphaalpha molecules promote the survival and differentiation of activated lymphocytes into memory CD8 T cells; memory precursors can be identified among primary effector cells and are selected for survival and differentiation by CD8alphaalpha PMID: 15105501
    48. The stronger affinity of the TL Ag for CD8alphaalpha is largely mediated by three amino acids on exposed loops of the conserved alpha3 domain. PMID: 15749886
    49. CD8alpha-CD11b+ dendritic cells in the mesenteric lymph nodes play a critical role for induction of cross-tolerance to dietary proteins PMID: 15774618
    50. results demonstrate epigenetic control of the Cd8 loci and identify MAZR as an important regulator of Cd8 expression PMID: 16491076

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 数据库链接：

    KEGG: mmu:12525

    STRING: 10090.ENSMUSP00000068123

    UniGene: Mm.1858