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  4. Recombinant Mouse Vesicle-associated membrane protein 8 (Vamp8)

Recombinant Mouse Vesicle-associated membrane protein 8 (Vamp8)

  • 货号：
    CSB-CF025786MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Vamp8; Vesicle-associated membrane protein 8; VAMP-8; Endobrevin; Edb
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-101
  • 氨基酸序列
    MEEASGSAGNDRVRNLQSEVEGVKNIMTQNVERILSRGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVIICVIVLIIVILIILFATGTIPT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism.
  • 基因功能参考文献：
    1. in addition to a secretory role, VAMP8-mediates trafficking of NOX2 to endosomes and phagosomes and this promotes induction of cytolytic T cell immune responses PMID: 28688576
    2. This report highlights the importance of the VAMP8 secretory machinery in facilitating mucus release from intestinal goblet cells and the dire consequences that occur during disease pathogenesis if these pathways are not functional. PMID: 28974617
    3. acute inhibition of VAMP8-mediated secretion during pancreatitis triggers intracellular trypsin accumulation and loss of the early endosomal compartment PMID: 28242757
    4. These findings reveal a role for VAMP8 in LC3-associated phagocytosis and highlight a novel mechanism exploited by L. major promastigotes to interfere with the host antimicrobial machinery PMID: 27280768
    5. VAMP8-mediated secretion is dependent on anterograde endosomal trafficking. PMID: 25138214
    6. Leishmania evades host immunity by GP63-mediated VAMP8 cleavage. PMID: 23870310
    7. VAMP8 mediates insulin granule recruitment to the plasma membrane, which partly accounts for GLP-1 potentiation of glucose-stimulated insulin secretion. PMID: 22841572
    8. VAMP8 knockout mice were used to show that the SNARE machinery plays a critical role in the process of granule homotypic fusion. PMID: 22450911
    9. VAMP8 regulates mucin granule exocytosis in airway goblet cells, and reduction of its expression may provide a novel therapeutic target to ameliorate airway mucus obstruction in lung diseases PMID: 22144578
    10. that granule-to-granule fusion is regulated by VAMP8 containing SNARE complexes distinct from those that regulate primary granule fusion. PMID: 21733851
    11. data demonstrate that, in the absence of VAMP8, the relative subcellular distribution of GLUT4 is altered, resulting in increased sarcolemma levels that can account for increased glucose clearance and insulin sensitivity PMID: 20876717
    12. VAMP8 mediates the regulated fusion of AQP2-positive vesicles with the plasma membrane. PMID: 19841070
    13. VAMP8 has a role in regulated exocytosis of pancreatic acinar cells by serving as a v-SNARE of zymogen granules PMID: 15363411
    14. VAMP-8 is required for release from dense core granules, alpha granules, and lysosomes. PMID: 17065550
    15. These results suggest that VAMP8 may act as a v-SNARE for regulated secretion of the entire exocrine system. PMID: 17215514
    16. VAMP-8 segregates secretory lysosomal granule exocytosis in mast cells from cytokine/chemokine molecular trafficking pathways. PMID: 18203950
    17. Functionally inhibits phagocytosis in dendritic cells upon exposure to bacteria PMID: 18292538
    18. VAMP8 is the zymogen granule-SNARE that mediates basolateral exocytosis in alcoholic pancreatitis. In addition, VAMP8 is critical for zymogen granule-zymogen granule homotypic fusion. PMID: 18535671
    19. VAMP8 has a specific function in the thymic stroma affecting the proliferation and apoptosis of T lymphocytes during maturation in the thymus. PMID: 18923845
    20. these results highlight the role of caspases in regulating VAMP-8 expression and subsequently phagocytosis during maturation of dendritic cells. PMID: 19607812
    21. Results clearly show that VAMP8 is one of the v-SNARE that regulate the lytic ability of CTL by influencing the ability of the lytic granules to fuse with the plasma membrane and release its contents. PMID: 19830729

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  • 亚细胞定位：
    Lysosome membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein. Early endosome membrane; Single-pass type IV membrane protein. Cell membrane; Single-pass type IV membrane protein. Zymogen granule membrane; Single-pass type IV membrane protein.
  • 蛋白家族：
    Synaptobrevin family
  • 组织特异性：
    Expressed abundantly in the kidney, less in the liver, brain, kidney, heart, lung, pancreas and placenta.
  • 数据库链接：

    KEGG: mmu:22320

    STRING: 10090.ENSMUSP00000059501

    UniGene: Mm.1838