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  4. Recombinant Rat Beta-secretase 1 (Bace1)

Recombinant Rat Beta-secretase 1 (Bace1)

  • 中文名称：
    Recombinant Rat Beta-secretase 1(Bace1)
  • 货号：
    CSB-CF002524RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Bace1
  • Uniprot No.：
  • 别名：
    Bace1; Bace; Beta-secretase 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    46-501
  • 氨基酸序列
    ETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKSVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNIFSLQLCGAGFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1.
  • 基因功能参考文献：
    1. Study utilizing glutamate excitotoxicity model in rat cortical primary cell cultures suggests for the first time a more direct action of BACE1 on the hyperphosphorylation state of tau (implemented in Alzheimer's disease pathogenesis) that is mediated through cPLA2 and desaturases SCD1 and FADS6 modulation during glutamate excitotoxicity. PMID: 30251689
    2. Beta-site APP cleaving enzyme 1 knockout (Bace1-/-) mice and rats differed in body weight, spontaneous locomotor activity, and prepulse inhibition of startle. PMID: 28281673
    3. miR-9 plays an important role in regulating the process of dementia induced by occlusion of the bilateral common carotid artery in rats by increasing BACE1 expression via downregulation of CREB. PMID: 28683457
    4. beta-site APP cleaving enzyme 1 (BACE1) is essential for amyloid beta protein production. We discovered that A673V mutation shifted the BACE1 cleavage site from the Glu(11) to the Asp(1) site, resulting in much higher C99 level and C99/C89 ratio. PMID: 28626014
    5. as an endogenous regulator of BACE1 protein, miR-124 may play a role in Alzheimer's disease onset induced by Chronic cerebral hypoperfusion. PMID: 26984601
    6. data indicate that Egr-1 promotes Abeta synthesis via transcriptional activation of BACE-1 and suggest that Egr-1 plays role in activation of BACE-1 and acceleration of Abeta synthesis in AD brain. PMID: 27576688
    7. This study demonstrated that the downregulation of BACE1 in Rat Medial Temporal Lobe Cortex Following Transient Global Brain ischemia. PMID: 26890784
    8. Lipid alterations induced by oxygen and glucose deprivation enhance beta-secretase 1 activity. PMID: 27022655
    9. Abeta oligomers induce BACE1 elevation via a post-translational mechanism involving its altered subcellular distribution in neurons. PMID: 26552445
    10. CHIP stabilizes amyloid precursor protein via proteasomal degradation and p53-mediated trans-repression of BACE1. PMID: 25773675
    11. It is suggested that increased corticosterone levels associated to stress lead to increase BACE transcription both through epigenetic mechanisms and activation of JNK. PMID: 22631614
    12. the levels of BACE1 were increased in the cortex and hippocampus of congestive heart failure rats PMID: 23127855
    13. Biochemical inhibition of the acetyltransferases ATase1 and ATase2 reduces beta-secretase (BACE1) levels and Abeta generation. PMID: 22267734
    14. It is upregulated by AGEs/RAGE complex via NF-kappaB pathway activation. PMID: 20638753
    15. These results demonstrate a spatial separation between APP and BACE1 during surface-to-endosome transport, suggesting subcellular trafficking as a regulatory mechanism for this proteolytic processing step. PMID: 21825135
    16. Data show that astrocytes possess beta-secretase activity and produce amyloid-beta because of the activity of BACE2, but not BACE1, the expression of which is blocked at the translational level. PMID: 21073551
    17. Data indicate that IGF-1-induced reduction of BACE-1 might involve the PI3-K/Akt and MAPK/ERK1/2 signaling pathways. PMID: 20821260
    18. Ovariectomy increases BACE1 expression and decreases neprilysin expression in hippocampus. PMID: 20137687
    19. BACE1-Fc chimera protein cleaved the A-ST6Gal I fusion protein, or ST6Gal I-derived peptide, between Leu(37) and Gln(38), suggesting that an initial cleavage product by BACE1 was three amino acids longer than the secreted ST6Gal I. PMID: 12473667
    20. promoter activities of deletion mutants suggests the presence of activators of BACE1 transcription between bases -514 to -753 and of suppressor elements between bases -754 and -1541. PMID: 12815710
    21. Beta-secretase is stimulated following brain injuries and may suggest a mechanism for the temporal increase of Abeta levels observed in patients with brain trauma. PMID: 15057522
    22. BACE1 expression reduces the activity of SOD1 in cells consistent with direct competition for available copper chaperone for superoxide dismutase-1 (CCS) as overexpression of CCS restores SOD1 activity. PMID: 15722349
    23. Results describe the functional domains of the BACE1 and BACE2 promoters and mechanisms of transcriptional suppression of the BACE2 promoter in normal neuronal cells. PMID: 16757811
    24. The conditioned medium from palmitic acid-treated astrocytes caused BACE1 upregulation in cortical neurons. PMID: 16904262
    25. Biological role of BACE in synapse function and plasticity as well as a potential mechanism whereby reduced neuronal activity or metabolism could lead to amyloid overproduction in synaptic terminals. PMID: 17206602
    26. Mitochondrial respiratory inhibition and oxidative stress facilitate BACE expression in vivo. Plaque constituents such as Fe(3+) and Abeta42f may participate in a self-enforcing cycle of amyloidogenesis via BACE upregulation. PMID: 17429617
    27. BACE1-gamma-secretase cleavages release the intracellular domain of beta2, which increases mRNA and protein levels of the pore-forming Na(v)1.1 alpha-subunit in neuroblastoma cells. PMID: 17576410
    28. wild-type or Swedish mutated betaAPP overexpression modulates BACE1 promoter transactivation and activity via an NFkappaB-dependent pathway. PMID: 18263584
    29. analysis of translation of BACE1 mRNA PMID: 19106624
    30. level of total BACE1 protein & beta-amyloid protein in the brain was increased in chronic cerebral hypoperfusion condition PMID: 19123057
    31. The early post-hypoxic up-regulation of BACE1 depends on the production of reactive oxygen species mediated by the sudden interruption of the mitochondrial electron transport chain. PMID: 19196431
    32. Enteropeptidase is a BACE1 substrate in vivo. PMID: 19734625

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Membrane raft. Lysosome. Late endosome. Early endosome. Recycling endosome. Cell projection, axon. Cell projection, dendrite.
  • 蛋白家族：
    Peptidase A1 family
  • 数据库链接：

    KEGG: rno:29392

    STRING: 10116.ENSRNOP00000022796

    UniGene: Rn.207201