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  4. Recombinant Rat Catechol O-methyltransferase (Comt)

Recombinant Rat Catechol O-methyltransferase (Comt)

  • 货号：
    CSB-CF005779RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Comt; Catechol O-methyltransferase
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-264
  • 氨基酸序列
    MPLAAVSLGLLLLALLLLLRHLGWGLVTIFWFEYVLQPVHNLIMGDTKEQRILRYVQQNAKPGDPQSVLEAIDTYCTQKEWAMNVGDAKGQIMDAVIREYSPSLVLELGAYCGYSAVRMARLLQPGARLLTMEMNPDYAAITQQMLNFAGLQDKVTILNGASQDLIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEKCGLLRKGTVLLADNVIVPGTPDFLAYVRGSSSFECTHYSSYLEYMKVVDGLEKAIYQGPSSPDKS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
  • 基因功能参考文献：
    1. The discovered changes in the expression level of catechol-o-methyltransferase , mineralocorticoid receptor , and b-subunit of epithelial sodium channel confirm their involvement in increased sympathetic stimulation of the of hypertensive rats PMID: 27215035
    2. A higher hepatic COMT activity was found in the mineral water group compared with the tap water and tap-water-plus-NaCl groups. On the other hand, adrenal gland COMT mRNA expression decreased in the MW group compared to TW group. PMID: 25560240
    3. COMT inhibition results in increased pain sensitivity and production of proinflammatory mediators. PMID: 24727346
    4. Our findings suggest that COMT regulates dorsal hippocampal neurochemistry and modulates hippocampus-dependent behaviours. PMID: 22815336
    5. ZNF804a regulates expression of the schizophrenia-associated genes PRSS16, COMT, PDE4B, and DRD2 PMID: 22384243
    6. decreased COMT activity was associated with some changes in feeding microstructure in rats and mice PMID: 21851556
    7. investigation of substrate specificity and metabolite profiles of COMT from liver cytosol; studies include molecular modeling PMID: 22071171
    8. analysis of orientation and cellular distribution of membrane-bound catechol-O-methyltransferase in cortical neurons PMID: 21846718
    9. In rat subarachnoid hemorrhage model could induce the expression of COMT in the rat striatum in the early stage. PMID: 21116937
    10. Reduction of COMT enzyme activity, and thereby possible accumulation of catecholamines, inhibits spinal nociceptive activity and attenuates the expression of spinal LTP. PMID: 20219633
    11. significant effects of hormone replacement and gonadectomy on catechol-O-methyltransferase and monoamine oxidase isoforms in both striatum and cortex PMID: 19909795
    12. These results suggest that the decreased expression of COMT may be an important factor leading to the development of hypertension. PMID: 19966533
    13. Contrary to expectations that COMT would be expressed predominantly in non-neuronal cells, the present study shows that neurons are the main cell populations expressing COMT mRNA in the rat forebrain. PMID: 12535946
    14. liver membrane-bound-catechol-O-methyltransferase may be a relevant factor in blood pressure regulation in rats PMID: 14714585
    15. Strong COMT mRNA expression was observed in the suprachiasmatic nucleus throughout its rostrocaudal extent at postnatal day 1 (P1) and P2, and the mRNA levels decreased gradually until P16. PMID: 15167541
    16. Crystals diffract to 1.6 A resolution on a synchrotron-radiation source and belong to the monoclinic space group P2(1). PMID: 16508109
    17. These data provide the first direct evidence that low COMT activity leads to increased pain sensitivity via a beta(2/3)-adrenergic mechanism. PMID: 17084978
    18. Suppression of catechol-O-methyltransferase activities, decrease in degradation of norepinephrine (NE), and increase in NE release by blunting of alpha(2)-AR function may be involved in salt-sensitive hypertension in Dahl salt-sensitive rats. PMID: 17510509
    19. Catechol-O-methyltransferase activity and protein expression were increased in the substantia nigra after inflammation induced by lipopolysaccharides. PMID: 17573159
    20. Modulation of COMT activity may play a role in the regulation of myometrial contractility and cervical ripening during pregnancy. PMID: 18042640
    21. pI differences distinguishing between the various COMT forms are due to some as yet unidentified structural modification(s) PMID: 18831714
    22. This work reports the first crystallographic study of Rat COMT complexed with non-nitrocatechol inhibitor. PMID: 19056347
    23. The crystal structures of the Apo and Holo form of rat catechol-O-methyltransferase were determined and compared. PMID: 19111934

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  • 亚细胞定位：
    [Isoform Soluble]: Cytoplasm.; [Isoform Membrane-bound]: Cell membrane; Single-pass type II membrane protein; Extracellular side.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Cation-dependent O-methyltransferase family
  • 数据库链接：

    KEGG: rno:24267

    STRING: 10116.ENSRNOP00000043148

    UniGene: Rn.220