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  4. Recombinant Rat Endothelin-1 receptor (Ednra)

Recombinant Rat Endothelin-1 receptor (Ednra)

  • 货号：
    CSB-CF007403RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Ednra; Endothelin-1 receptor; Endothelin receptor type A; ET-A; ET-AR
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    21-426
  • 氨基酸序列
    DNAERYSANLSSHVEDFTPFPGTEFNFLGTTLQPPNLALPSNGSMHGYCPQQTKITTAFK YINTVISCTIFIVGMVGNATLLRIIYQNKCMRNGPNALIASLALGDLIYVVIDLPINVFK LLAGRWPFDHNDFGVFLCKLFPFLQKSSVGITVLNLCALSVDRYRAVASWSRVQGIGIPL ITAIEIVSIWILSFILAIPEAIGFVMVPFEYKGEQHRTCMLNATTKFMEFYQDVKDWWLF GFYFCMPLVCTAIFYTLMTCEMLNRRNGSLRIALSEHLKQRREVAKTVFCLVVIFALCWF PLHLSRILKKTVYDEMDKNRCELLSFLLLMDYIGINLATMNSCINPIALYFVSKKFKNCF QSCLCCCCHQSKSLMTSVPMNGTSIQWKNQEQNHNTERSSHKDSMN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
  • 基因功能参考文献：
    1. Homocysteine up-regulates ETA receptor expression via the Sirt1/ERK1/2 signaling pathway in vascular smooth muscle cells. PMID: 28579512
    2. The results presented herein show that ETB receptors (R) have much weaker Ca(2+)-sensitizing effect than ETA-R and that ETB-R appear to be more dependent on Ca(2+) influx presumably through voltage-gated Ca(2+) channels (VGCC). PMID: 28757442
    3. endocytosis of ETA receptors following ligand binding preserves their active state by protection against antagonist. PMID: 28412414
    4. Expression of smooth muscle proteins and both ETA and ETB by the endovascular trophoblast enable it to contract upon activation of both receptors by ET1. PMID: 27697218
    5. both ETA and ETB receptor expression in the renal cortex were significantly attenuated by ovariectomy, and this reduction was not evident in OVX+E2 rats. SIGNIFICANCE: These data suggest that ovarian hormones regulate ET receptor expression and may contribute to sex differences in cardiovascular and renal health. PMID: 26776836
    6. ETaR siRNA is capable of down-regulating the expression of inflammatory and transcription factors among endothelial cells treated with hypoxia. PMID: 27997891
    7. Confirm the presence of ETA-ETB receptor cross-talk in the rat basilar artery. PMID: 26528589
    8. data suggest that ET receptors may be an important actor in diabetes-related liver damage. PMID: 24918345
    9. ET-1/ET-3 induce down-regulation of ET-A receptor mrna in coronary arteries, but do affect protein levels or vasoconstrictor response. PMID: 25891848
    10. Data show that the protein expression of the endothelin receptors ETA and ETB was significantly elevated in male growth-restricted offspring. PMID: 26459423
    11. ETA receptor-dependent vasoconstriction is missing and angiotensin II-dependent vasoconstriction is attenuated in blood pressure-lowering treatment with atrasentan in transgenic rats PMID: 25255392
    12. Cirrhotic rats with diabetes showed higher intrahepatic ET-1 vasoresponsiveness than normoglycemic cirrhotic rats. This effect may be related, at least in part, to intrahepatic ETA R receptor and pERK over-expression. PMID: 24636620
    13. The aim was to quantify the density of ETA and ETB receptors in cardiopulmonary tissue from pulmonary arterial hypertension patients and the monocrotaline (MCT) rat. PMID: 24582810
    14. The results of this study demonstrate that expression of ETA receptors does not change with postnatal development. PMID: 24815227
    15. Endothelin-A receptor antagonist BQ-123 impeded the formation and spread of pentylenetetrazole-induced seizures to a great degree. PMID: 24449761
    16. Data implicate endothelin ETA receptors in baroreflex and vascular derangements which predispose to the hypertensive effect of cyclosporine A. PMID: 24486390
    17. In rat pulmonary small arteries, ET-1 induces receptor-operated calcium influx via the ETA receptor, and that this influx interacts with InsP3-receptor activation. PMID: 24157978
    18. Report effect of antidiabetic agents on endothelin receptor subtype(s) regulation/binding in type 1 diabetic rat hearts. PMID: 24144054
    19. The inner medullary collecting ducts in the nephron segment from male and female rats have similar ETB expression, whereas male rats have significantly higher ETA expression. PMID: 23946290
    20. The results suggest that the ET(A)/ET(B) receptors play an important role in cerebral vasospasm after subarachnoid hemorrhage PMID: 23351051
    21. In rat trigeminal ganglion neurons, ETA activation leads to TRPV1 hyperactivation via PKC activation. PMID: 23396520
    22. Secondhand smoke exposure induces transcriptional upregulation of the 5-HT(2A), ET(B) and ET(A) receptors in rat bronchial smooth muscle cells. PMID: 22952915
    23. Arterial smooth muscle ETA-receptor function displays agonist-dependent aspects; this involves roles of amino acids on position 6 and 7 of the endothelin sequence PMID: 22406300
    24. Our results demonstrated that ETA-R blockage is more effective in inhibiting free radical generation and improving heart antioxidant properties than ETB-R blockage under oxidative stress. PMID: 23047940
    25. expression in articular cartilage is increased in response to both surgical induction of osteoarthritis and TGFalpha treatment PMID: 22407503
    26. ET(A)-ET(B) receptor cross-talk is present in the rat basilar artery PMID: 22258095
    27. Marked decrease of mossy fiber endothelin receptor A following ischemia may contribute to glutamate release from mossy fiber terminals, thus enhancing excitotoxic effects on their cornu ammonis synaptic targets. PMID: 21801590
    28. Downregulation of the ET-A receptor impacts the potential influence of the endothelin system on the intra-axonal cascade of events underlying diffuse axonal injury. PMID: 21801594
    29. Antagonism of endothelin receptor A, but not endothelin receptor B, improves behavioral outcome following traumatic brain injury. PMID: 21801595
    30. Activation of ETA receptors contributes to the impaired responsiveness of renal mechanosensory nerves in rats with congestive heart failure by a mechanism(s) at the renal sensory nerve endings. PMID: 20628427
    31. results demonstrate an important role of endothelin in mediating diuretic responses to intramedullary infusion of hyperosmotic NaCl. Data suggest ET(A) and ET(B) receptors are both required for the full diuretic and natriuretic actions of endothelin. PMID: 20844020
    32. findings suggest that endothelins, acting through ET(A) and/or ET(B) receptors, may play an important role in mediating pain resulting from activation of most trigeminal nerve branches PMID: 20450894
    33. Over-expression of ET(A) and leptin account-ing for the testis dysfunction is likely to be mediated by pPKCvarepsilon in the iso-proterenol treated rats. PMID: 20307649
    34. There was no correlation of ETRA expression with alveolar-arterial oxygen gradient in rats with hepatopulmonary syndrome. PMID: 16463654
    35. Altered expression of EDNRA is an early event in lung morphogenesis in the nitrofen model. PMID: 19851775
    36. GRK2 is a key regulator of ET(A)R responsiveness in resistance arteries. PMID: 19748906
    37. ET(A) and/or ET(B) receptors play an important role in I/R-induced mucosal dysfunction PMID: 11897624
    38. ET-1-induced venoconstriction was mediated by ET(A) receptors in mesenteric blood vessels from deoxycorticosterone acetate (DOCA-salt) hypertensive and normotensive control rats PMID: 11910302
    39. the ET(A) receptor may be present in the capillary wall of the rat skeletal muscle, including the endothelium PMID: 12074645
    40. Expression of the ETA receptor was not affected by cloprostenol. The reversal of PGF(2alpha)'s luteolytic effect by an ETA receptor antagonist suggests that ET-1 may take part in the PGF(2alpha)-induced luteolysis in the rat. PMID: 12211063
    41. ET(A) response in hepatic stellate cells is regulated by protein kinase A (PKA)-dependent receptor phosphorylation and internalization. PMID: 12297833
    42. in endotheline-1 stimulated increase in calcium signaling in astrocytes, ET(A) was expressed more than ET(B) suggesting that ET(A) is the major receptor PMID: 12464391
    43. ETA receptor blockade enhances kidney tubular cell proliferation, cyst number, and size and reduces renal circulation. PMID: 12538737
    44. ET(A) receptors are expressed and functionally coupled to rise of [Ca(2+)](i) in H9c2 cardiomyoblasts. PMID: 12628492
    45. A functional ETA receptor activating a Ca2+-dependent pathway is expressed in Henle's loop and this ETA-induced calcium signaling is impaired in two strains of genetically hypertensive rats. PMID: 12631344
    46. Upregulation in the immunoexpression and number of astrocytes expressing the ET(B)R in both gray and white matter and near disappearance of ET(B)R-IR in ependymal cells and ET(A)R-IR in primary afferent fibers following spinal cord injury PMID: 12668144
    47. that endothelin ET(A) receptors located on the sympathetic nerve terminals play a role in the inhibition of noradrenaline release from the rat stomach; inhibition is carried out by pertussis toxin-sensitive and indomethacin-insensitive mechanisms PMID: 12686728
    48. ET receptor subtype (ET(A) and ET(B)) mRNAs were shown to be higher in bladders of 3-week- compared to 3-month- or 22-month-old rats. PMID: 12700883
    49. cardiac nuclei possess both endothelin A and endothelin B receptor subtypes, which are functional with respect to ligand binding and are coupled to signaling mechanisms within the nuclear membrane PMID: 12756260
    50. in acute necrotizing pancreatitis, associated development of microcirculatory failure, inflammation, and parenchymal injury is caused by ETs coupling onto the ETA receptor PMID: 12799311

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Endothelin receptor subfamily, EDNRA sub-subfamily
  • 组织特异性：
    Predominantly expressed in vascular smooth muscle cells of a variety of issues, bronchial smooth muscle cells, myocardium, and the pituitary gland.
  • 数据库链接：

    KEGG: rno:24326

    STRING: 10116.ENSRNOP00000054967

    UniGene: Rn.10915