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  4. Recombinant Rat Gamma-glutamyltranspeptidase 1 (Ggt1)

Recombinant Rat Gamma-glutamyltranspeptidase 1 (Ggt1)

  • 货号：
    CSB-CF009394RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Ggt1
  • Uniprot No.：
  • 别名：
    Ggt1; Ggt; Glutathione hydrolase 1 proenzyme; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1; GGT 1; Leukotriene-C4 hydrolase; CD antigen CD224
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-379
  • 氨基酸序列
    MKNRFLVLGLVAVVLVFVIIGLCIWLPTTSGKPDHVYSRAAVATDAKRCSEIGRDMLQEGGSVVDAAIASLLCMGLINAHSMGIGGGLFFTIYNSTTRKAEVINAREMAPRLANTSMFNNSKDSEEGGLSVAVPGEIRGYELAHQRHGRLPWARLFQPSIQLARHGFPVGKGLARALDKKRDIIEKTPALCEVFCRQGKVLQEGETVTMPKLADTLQILAQEGARAFYNGSLTAQIVKDIQEAGGIMTVEDLNNYRAEVIEHPMSIGLGDSTLYVPSAPLSGPVLILILNILKGYNFSPKSVATPEQKALTYHRIVEAFRFAYAKRTMLGDPKFVDVSQVIRNMSSEFYATQLRARITDETTHPTAYYEPEFYLPDDGG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.
  • 基因功能参考文献：
    1. an extensive ductular reaction in advanced Hepatocellular carcinoma (HCC) and a strong correlation between PCK7 and levels of gammaGT and the poor prognosis and aggressiveness of HCC, is reported. PMID: 27525410
    2. These data demonstrate the important role of endothelial GGT activity in mediating the vasorelaxant effect of S-nitrosoglutathione in rat aorta . PMID: 22984412
    3. The expression of gamma-glutamyltransferase in rat colon carcinoma cells is distinctly regulated during differentiation and oxidative stress PMID: 12030384
    4. Radiation of CC531 colon carcinoma cells blocked cell proliferation, increased cellular size, initiated apoptosis and upregulated GGT activity and protein levels in a dose- and time-related manner. PMID: 16386375
    5. With the co-existence of inflammation and hypoxia in the brain after ischemic stroke, dynamic changes of gammaGT activity reflect evolving changes of endothelial function. PMID: 16973162
    6. Data suggest that extracellular glutathione elicits gamma-glutamyl transpeptidase and ROS-dependent transactivation of tyrosine kinase signaling that upregulates potassium channel activity or expression via redox-mediated mechanisms. PMID: 19419996
    7. The acylation reaction of GGT by gamma-glutamyl anilides can apparently be accelerated by either electron-withdrawing or electron-donating substituents, suggesting a concerted mechanism involving kinetic general acid catalysis of aniline departure. PMID: 11601992

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Gamma-glutamyltransferase family
  • 组织特异性：
    Detected in adult kidney and mammary gland, and in fetal liver.
  • 数据库链接：

    KEGG: rno:116568

    UniGene: Rn.10010