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  4. Recombinant Rat Gap junction alpha-5 protein (Gja5)

Recombinant Rat Gap junction alpha-5 protein (Gja5)

  • 中文名称：
    大鼠Gja5重组蛋白
  • 货号：
    CSB-CF009448RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Gja5
  • Uniprot No.：
  • 别名：
    Gja5; Cxn-40; Gap junction alpha-5 protein; Connexin-40; Cx40
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-356
  • 氨基酸序列
    GDWSFLGEFLEEVHKHSTVIGKVWLTVLFIFRMLVLGTAAESSWGDEQADFRCDTIQPGC QNVCYDQAFPISHIRYWVLQIIFVSTPSLVYMGHAMHTVRMQEKQKLREAEKAKEAGGTG TYEYLAEKAELSCWKEVNGKIVLQGTLLNTYVCTILIRTAMEVAFMVGQYLLYGIFLDTL HVCRRSPCPHPVNCYVSRPTEKNVFIVFMMAVAGLSLFLSLAELYHLGWKKIRQRLAKSR QGDKHQLLGPSTSLVQGLTPPPDFNQCLKNSPDEKFFSDFSNNMGSRKNPDPLATEEVPN QEQIPEEGFIHTQYGQKPEQPSGASAGHRFPQGYHSDKRRLSKASSKARSDDLSV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • 基因功能参考文献：
    1. The A96S, G38D, and P88S Cx40 mutations slow conduction and increased the propensity for inducible AF. PMID: 28457700
    2. Lipopolysaccharide impairs permeability of pulmonary microvascular endothelial cells via Connexin40/protein kinase C/nfkappaB pathway. PMID: 28870649
    3. This study demonstrated the Cx40 was upregulated after traumatic brain injury in a linear correlated manner with increased oxidative stress. PMID: 28157110
    4. data are consistent with contributions of G2 and D3 residues to Cx40 channel pore structure, but not with postulated hydrophobic W4 intermolecular interactions; results indicate an absolute requirement for an amphipathic W3/4 residue; alternatively hypothesize that the connexin D2/3-W3/4 locus interacts with the highly conserved FIFR M1 motif to stabilize the NT domain within the pore PMID: 27304225
    5. Data show that the ratio of peripheral blood CD4(+)/CD8(+) T lymphocytes and the levels of connexin-40 (Cx40), interleukins IL-2, IL-4 and IL-6 are significantly elevated in SH rats. PMID: 26927369
    6. Cx40 expression in mesangial cells is related to mesangial cell regeneration PMID: 22945766
    7. The expressions of Cx40 and HCN4 increase obviously after coculturing bone marrow mesenchymal stem cells with sinoatrial node tissue. PMID: 22403875
    8. Valsartan and U0126 were equivalent on attenuating atrial fibrosis and Cx40 remodeling by inhibiting ERK pathways at different levels. PMID: 22336507
    9. three-dimensional molecular topography and calcium-sensitive conformational changes of Connexin40 hemichannels PMID: 21543330
    10. The demonstrated that in the endothelium of the native vein, the gap-junctional spot numbers (GJSNs) and the total gap-junctional areas (TGJAs) of Cx40 were lower than those of the thoracic aorta. PMID: 20805582
    11. Data show heterogeneous expression of the 3 Cx isoforms Cx43, Cx45, and Cx40 in heart. PMID: 17584645
    12. The total amount of connexins and the ratio of phosphorylated/non-phosphorylated isoforms gradually increased during the re-establishment of intercellular communication in heart myocytes. PMID: 11889564
    13. Kinetics of Cx40 voltage gating. There was a temporal correlation between development of conduction delay or block and the inactivation phase of junctional conductance. PMID: 14693688
    14. role for Cx40 in EDHF-mediated dilation of rat mesenteric arteries. PMID: 16037574
    15. data suggest that the HKH motif at positions 15-17 is important to the conformational structure of the putative voltage sensor and spermine receptor of Cx40 PMID: 16284078
    16. Adrenergic stimulation of cardiomyocytes can enhance connexin expression thereby increasing cellular coupling, indicating a possible role for catecholamines in the regulation of cardiac gap junction expression in cardiac disease. PMID: 16352648
    17. Structural remodelling of gap junction connexin proteins may partly underlie electrophysiological defects in STZ-induced diabetic rat sinus atrial node. PMID: 17632690
    18. Report regulation of connexin 40 gene expression during skeletal muscle regeneration in the adult rat. PMID: 19129462
    19. the selectivities of junctions formed by cells coexpressing Cx40 and Cx43 vary considerably, reflecting both their relative expression levels and phosphorylation-dependent regulation PMID: 19465552
    20. Cx40/43 can regulate the superior mesenteric artery endothelium-dependent vascular contractive response through [Ca2+]i of vascular smooth muscle cells after hemorrhagic shock. PMID: 19588758

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
  • 蛋白家族：
    Connexin family, Alpha-type (group II) subfamily
  • 组织特异性：
    Highly expressed in lung.
  • 数据库链接：

    KEGG: rno:50563

    STRING: 10116.ENSRNOP00000023488

    UniGene: Rn.88300