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  4. Recombinant Rat High affinity nerve growth factor receptor (Ntrk1)

Recombinant Rat High affinity nerve growth factor receptor (Ntrk1)

  • 货号：
    CSB-CF016133RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Ntrk1; Trk; Trka; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; Slow nerve growth factor receptor; p140-TrkA; Trk-A
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    33-799
  • 氨基酸序列
    AASCRETCCPVGPSGLRCTRAGTLNTLRGLRGAGNLTELYVENQRDLQRLEFEDLQGLGELRSLTIVKSGLRFVAPDAFHFTPRLSHLNLSSNALESLSWKTVQGLSLQDLTLSGNPLHCSCALLWLQRWEQEDLCGVYTQKLQGSGSGDQFLPLGHNNSCGVPSVKIQMPNDSVEVGDDVFLQCQVEGQALQQADWILTELEGTATMKKSGDLPSLGLTLVNVTSDLNKKNVTCWAENDVGRAEVSVQVSVSFPASVHLGKAVEQHHWCIPFSVDGQPAPSLRWFFNGSVLNETSFIFTQFLESALTNETMRHGCLRLNQPTHVNNGNYTLLAANPYGQAAASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSRDPVEKKDETPFGVSVAVGLAVSAALFLSALLLVLNKCGQRSKFGINRPAVLAPEDGLAMSLHFMTLGGSSLSPTEGKGSGLQGHIMENPQYFSDTCVHHIKRQDIILKWELGEGAFGKVFLAECYNLLNDQDKMLVAVKALKETSENARQDFHREAELLTMLQHQHIVRFFGVCTEGGPLLMVFEYMRHGDLNRFLRSHGPDAKLLAGGEDVAPGPLGLGQLLAVASQVAAGMVYLASLHFVHRDLATRNCLVGQGLVVKIGDFGMSRDIYSTDYYRVGGRTMLPIRWMPPESILYRKFSTESDVWSFGVVLWEIFTYGKQPWYQLSNTEAIECITQGRELERPRACPPDVYAIMRGCWQREPQQRLSMKDVHARLQALAQAPPSYLDVLG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand. Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
  • 基因功能参考文献：
    1. the findings of this in vivo/ex vivo study show the NGF ability to reduce the potential UV damage. Although the mechanism underneath this effect needs further investigation, these observations prospect the development of a pharmacological NGF-based therapy devoted to maintain cell function when exposed to phototoxic UV radiation. PMID: 29450621
    2. Foretinib protected neurons by suppressing both known degenerative pathways and a new pathway involving unliganded TrkA and transcriptional regulation of the proapoptotic BH3 family members. PMID: 28877995
    3. As a result, overexpression of PTP-MEG2 down-regulates NGF/TrkA signaling and blocks neurite outgrowth and differentiation PMID: 27655914
    4. The data suggest that elevated NTRK1 expression increases differentiation of neural stem cells into cholinergic neurons under stimulation of NGF PMID: 26509167
    5. Dimeric dipeptide mimetics of the nerve growth factor Loop 4 and Loop 1 activate TRKA with different patterns of intracellular signal transduction. PMID: 26642930
    6. Our data suggest that RabGEF1 is a negative regulator of TrkA-dependent neuronal differentiation and of NMDA receptor-mediated signaling activation in NGF-differentiated PC12 cells. PMID: 26588713
    7. The basal density of NGF and TrkA immunoreactivity detected in the paraventricular nucleus of the hypothalamus and hippocampal CA1, CA3 and DG did not change in P720 rats in comparison to P90 animals exposed to mild stress PMID: 25644424
    8. USP8 knockdown increases TrkA half-life, suggesting that the deubiquitinating activity of USP8 promotes TrkA degradation. PMID: 25662281
    9. Methylmercury causes neuronal cell death through the suppression of the TrkA pathway PMID: 25545984
    10. The majority of sensory dorsal root ganglia neurons innervating the subchondral bone of the distal femur were calcitonin gene-related peptide-IR and tyrosine receptor kinase A-IR PMID: 23821557
    11. Data indicate that NHE5 depletion reduces cell surface abundance of TrkA and nerve growth factor (NGF)-induced PI3K signaling. PMID: 24006492
    12. Data indicate that neuroendocrine long coiled-coil protein 2 (NECC2) distributes in caveolae, wherein it colocalizes with the TrkA receptor, and behaves as a caveolae-associated protein in neuroendocrine PC12 cells. PMID: 24040018
    13. Over-expression of SOCS2 in PC12 Tet-On cells increased total and surface TrkA expression. PMID: 24484474
    14. results suggest that NGF-trkA signaling is involved in mechanical allodynia in paclitaxel-induced neuropathy PMID: 24480438
    15. Data indicate that LIM homeobox 8 (Lhx8) regulates functions of cholinergic neurons by modulating tropomyosin receptor kinase A (TrkA) expression. PMID: 24265310
    16. Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons. PMID: 23928917
    17. The pathway of NGF-TrkA expressed in the small-size neurons, associated with neuropathic pain, was shifted to the NGF-p75 pathway expressed in the large-size neurons after RTX treatment, in association with neuropathic pain inhibition. PMID: 23615109
    18. TrkA participates in insulin signaling pathway in PC12 cells. PMID: 23749991
    19. Dissecting the roles of tyrosines 490 and 785 of TrkA protein in the induction of downstream protein phosphorylation using chimeric receptors. PMID: 23589303
    20. NGF and its TrkA receptor might be involved in the pathogenesis of chronic obstructive pulmonary disease mediated by alveolar macrophages. PMID: 23158009
    21. TrkA suppression disrupts cortical cholinergic transmission and associated attentional capacities. PMID: 23228124
    22. the neurite varicosities are intensely active in intracellular membrane trafficking, and play an important role in the degradation and accumulation of TrkA. PMID: 23100034
    23. neurotrophin binding to Trk initiates the recruitment of cytoplasmic dynein to signaling endosomes PMID: 23115187
    24. ectopic expression of TrkA may result in the promotion of neurotrophic effects or can influence neuronal plasticity in the absence of exogenous nerve growth factor in neuronal populations that naturally fail to respond to this factor. PMID: 22419059
    25. Spry4 regulates neurite outgrowth reducing not only the MAPK pathway but also restricting TrkA signaling and Rac1 activation in response to NGF PMID: 22384148
    26. The data indicated that TrkA and p75NTR partition into membrane rafts by different mechanisms, and that the fraction of TrkA that associates with detergent resistant membranes is internalized but does not directly form signaling endosomes. PMID: 22496904
    27. NGF receptors are localized in the mitochondrial compartment of undifferentiated human podocytes and in all tissues analyzed including rat central nervous system. PMID: 22138126
    28. Effects of castration on the expression of the NGF and TrkA in the vas deferens and accessory male genital glands of the rat. PMID: 22073361
    29. We found remarkable changes of the behavior of TrkA upon the application of NGF PMID: 21312342
    30. Immobilised DHEA pulls down TrkA and p75NTR receptors, and induces TrkA- and p75NTR-mediated signaling. RNA interference against TrkA receptors reverses the anti-apoptotic effect of DHEA. PMID: 21541365
    31. These results indicate that the ubiquitin-proteasome system potentiates neurite formation by regulating the stability of TrkA receptors. PMID: 21429296
    32. Data suggest that the effects of necdin deletion on the developing nervous system may depend on the relative expression of p75NTR and TrkA in the cells of particular regions of the nervous system. PMID: 21150695
    33. These data suggest that 4-methylcatechol might exert its antiapoptotic actions through NGF/TrkA system which may block NGF/p75(NTR) activation in pancreatic beta cells of hyperglycemic rats. PMID: 21295348
    34. Select PP2A heterotrimers oppose desensitization of the TrkA receptor tyrosine kinase, perhaps through dephosphorylation of inhibitory Ser/Thr phosphorylation sites on the receptor itself. PMID: 19029245
    35. Tiam1 mediates TrkA signaling and neurite outgrowth induced by NGF PMID: 20333299
    36. TrkA activity is neuroprotective, while p75NTR activity is neurotoxic through a paracrine mechanism in retinal neurodegeneration PMID: 20943663
    37. The results of this study suggested that colitis-induced primary afferent activation involved retrograde transport of TrkA but not TrkB from the distal colon to primary afferent neurons in dorsal root ganglia. PMID: 20638179
    38. Data show that unique neurotrophin receptor tumor profiles may determine tumor aggressiveness and chemoresistance. PMID: 19701634
    39. The key mutations along the NGF receptor interface are transmitted inside the cell to enable the generation of survival-selective recombinant NGF muteins that may represent novel pharmacologic lead agents for the amelioration of Alzheimer disease. PMID: 19762468
    40. A necdin-dependent shift from p75NTR to TrkA signaling results in loss of resistance to oxidant stress in PC12 cells. PMID: 19818769
    41. Distinctive features of Trk neurotrophin receptor transactivation by G protein-coupled receptors PMID: 11750876
    42. Data show that the primary effect of NGF treatment is a p75(NTR)-modulated decrease in nerve growth factor receptor transit time at the cell surface. PMID: 12055187
    43. TrkA is expressed in visual cortical neurones during postnatal development and in adulthood and that its pattern of expression is developmentally regulated. PMID: 12151805
    44. Chronic ethanol treatment results in a reduction in basal forebrain trkA receptor protein and mRNA that could compromise septohippocampal synaptic connections, cholinergic differentiation, and emergent functional abilities dependent on these properties. PMID: 12231238
    45. TrkA is activated by gangliosides inducing the release of neurotrophins, but less potently than TrkC PMID: 12388556
    46. Data show that expression of a chimeric receptor containing the juxtamembrane region of Musk and the kinase domain of TrkA restores presynaptic and postsynaptic differentiation in Musk-deficient mice. PMID: 12403715
    47. A strong, ischemia-induced up-regulation of TrkA immunoreactivity is observed in the majority of reactive astroglia population in the adjacent CA1 hippocampal region. PMID: 12849738
    48. Both TrkA- and TrkB-immunostained cells were widely distributed in the lateral, medial and spinal vestibular nuclei, and were less frequently seen in the superior vestibular nucleus, x and y subnuclei PMID: 12858046
    49. NGF rescue from growth factor withdrawal requires NGF signalling through the high-affinity tyrosine kinase receptor (TrkA). PMID: 12882321
    50. TrkA has a critical role in maintaining transcription in neuronally differentiated rat tumor cells PMID: 12909622

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
  • 组织特异性：
    Isoform Trka-II is primarily expressed in neuronal cells; isoform Trka-I is found in non-neuronal tissues.
  • 数据库链接：

    KEGG: rno:59109

    STRING: 10116.ENSRNOP00000018961

    UniGene: Rn.39098