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  4. Recombinant Rat Occludin (Ocln)

Recombinant Rat Occludin (Ocln)

  • 货号：
    CSB-CF016263RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Ocln; Occludin
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-523
  • 氨基酸序列
    MSVRPFESPPPYRPDEFKPNHYAPSNDMYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTS PPGVIRILSMLVIVMCIAVFACVASTLAWDRAYGTGIFGGSMNYPYGSGFGSYGGGFGGY GYGYGYGYGGYTDPRAAKGFLLAMAAFCFIASLVIFVTSVIRSGMSRTRRYYLIVIIVSA ILGIMVFIATIVYIMGVNPTAQASGSMYGSQIYTICSQFYTPGGTGLYVDQYLYHYCVVD PQEAIAIVLGFMIIVAFALIIVFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKN VSAGTQDMPPPPSDYAERVDSPMAYSSNGKVNGKRSYPDSLYKSPPLVPEVAQEIPLTLS VDDFRQPRYSSNDNLETPSKRTPTKGKAGKAKRTDPDHYETDYTTGGESCDELEEDWLRE YPPITSDQQRQLYKRNFDAGLQEYKSLLAELDEVNKELSRLDRELDDYREESEEYMAAAD EYNRLKQVKGSADYKSKKNYCKQLKSKLSHIKRMVGDYDRRKT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells.
  • 基因功能参考文献：
    1. blood occludin levels correlate well with the extent of blood brain barrie damage. PMID: 28079139
    2. Both upregulation of claudin-4 and downregulation of occludin might increase paracellular NaCl transport in the kidney, resulting in impaired pressure natriuresis in SS rats. PMID: 29179201
    3. Up-regulation of miR-144 could promote intestinal hyperpermeability and impair the protective effect of the epithelial barrier by directly targeting OCLN and ZO-1. PMID: 29258088
    4. We provide quantitative evidence that claudin-11 contributes significantly to Sertoli cell tight junction function in vitro. Interestingly, occludin, which is hormonally regulated but not implicated in infertility until late adulthood, is also a significant contributor to barrier function. PMID: 26585695
    5. Mechanical ventilation can activate c-Src by phosphorylation and increase the degradation of occluding. PMID: 25471013
    6. In severe acute pancreatitis, downregulated expression of alphaSNAP in intestinal epithelial cells leads to reduced occludin expression, apoptosis of intestinal epithelial cells, and increased permeability of the intestinal barrier. PMID: 25278303
    7. actin cytoskeletal dynamics is detrimental to METH-induced BBB dysfunction by increasing internalization of occludin. PMID: 24081143
    8. cells transfected with Cx43 siRNA and grown in N medium showed significant downregulation in occludin (78 +/- 8% of control) and ZO-1 (81 +/- 6% of control) expression, and exhibited increased cell monolayer permeability. PMID: 24008412
    9. Results identified a crucial role of occludin in submandibular epithelial cells, and more importantly, demonstrated that occludin was required to mediate TRPV1-modulated paracellular permeability. PMID: 23345400
    10. High temperature inhibited the proliferation of rat Sertoli cells in vitro, and decreased the expression of OCLN. PMID: 23297502
    11. Disruption of pulmonary epithelium induced by hyperoxia is due in part to decreased expression of occludin and zona occludens (ZO)-1 proteins. PMID: 22559818
    12. Reverse-flow perfusions of mesenteric venules showed numerous small gaps in occludin expression on the endothelium. Similar gaps were not seen in vessels perfused in the forward direction. PMID: 23417864
    13. data indicate the possibility that interleukin 6 might be involved in the downregulation of occludin expression and in the modulation of blood-testis barrier permeability that occur in rats undergoing autoimmune orchitis PMID: 23018187
    14. Occludin and Zo-1 were reduced in intestinal mucosa both in mRNA and protein levels in the rat tail-suspension model. PMID: 21336719
    15. Occludin protein loss and claudin-5 redistribution are detected in ischemic microvessels following middle cerebral artery occlusion. PMID: 22378877
    16. Levels of tight junction protein occludin are decreased in blood-brain barrier cells post-translationally following exposure to neurotoxicants. PMID: 20970449
    17. A calcium-activated potassium channel activator increases blood-brain tumor-barrier permeability by down-regulating the expression of occludin. PMID: 21334421
    18. In female rats, increasing age and 17beta-estradiol treatment alters the expression of ERalpha and distinct blood-brain barrier tight-junction protein isoforms (occludin and claudin-5) without altering functional paracellular permeability. PMID: 21192956
    19. the evidence presented herein suggests the implication of occludin and, therefore, of blood-brain barrier in the pathophysiology of extrahepatic cholestasis. PMID: 20170644
    20. There was a significant down-regulation in the expression of occludin abd JAM-1 after exposure to microwave radiation. PMID: 20180397
    21. The presence of occludin in the tight junctions at the time of implantation suggests that the paracellular pathway is impermeable to water and Na(+) at this time, and that the transport of such substances takes place via the transcellular pathway. PMID: 19555995
    22. present in the junctions of the olfactory epithelium, and in the endothelial cells in the blood vessels in the lamina propria of the olfactory mucosa PMID: 11751462
    23. After ligation of the common bile duct, the expression of this protein was elevated in the liver. PMID: 11845325
    24. diabetes-related changes in cerebral zonula occludin-1 expression (zonula occludin-1) PMID: 11958524
    25. Occludin was immunoreactive at distal and proximal complexes of early differentiating ameloblasts and at distal regions of differentiating odontoblasts. However, in more advanced stages, occludin was only evident at the proximal complex of ameloblasts. PMID: 15052661
    26. In vitro blood-brain barrier model studies revealed that the pericyte-derived multimeric angiopoietin-1/Tie-2 pathway induces occludin expression PMID: 15056293
    27. Taken collectively, the results reported herein support the notion that db-cAMP-induced TJ disruption was mediated by an induction of Itch protein expression, which in turn triggered the ubiquitination of occludin resulting in TJ disruption. PMID: 15605377
    28. The effect of matrix metalloproteinase(MMP) inhibitors on endothelial gap formation, occludin loss, and the ability of monocytes to pass the endothelium is reported. PMID: 17065217
    29. These findings indicate the opposite effects of the NMDA and AMPA/kainate receptors on occludin phosphorylation and disruption of the blood-brain barrier. PMID: 17245419
    30. Immunohistochemical expression of occludin and connexin 43 were decreased in the testis after vasal and epididymal ligation. PMID: 17825302
    31. The increase of BK-mediated BTB permeability is associated with the down-regulation of ZO-1, occludin, and claudin-5 and the rearrangement of F-actin;cAMP/PKA might be involved in the modulating process. PMID: 18183615
    32. Chronic ethanol ingestion impairs the airway epithelial barrier function through down-regulation and disruption of membrane localization of occludin and E-cadherin. PMID: 18279593
    33. Increased BBB permeability associated with peripheral inflammatory pain is promoted by the disruption of disulfide-bonded occludin oligomeric assemblies, which renders them incapable of forming an impermeant physical barrier to paracellular transport. PMID: 18647175
    34. The changes in occludin distribution and decreased expression of ZO-1 lead the reorganization of BBB-actin protein, which may be one of the mechanisms of permeability increasing of BBB following hypoxia-ischemia. PMID: 18706176
    35. Cardiopulmonary bypass markedly down-regulates the expression of occludin and ZO-1 proteins in intestinal mucosa of rats. PMID: 18855986
    36. a unique motif in the occludin sequence that is involved in the regulation of ZO-1 binding by reversible phosphorylation of specific Tyr residues. PMID: 19017651
    37. Transient focal ischemia increased the tyrosine phosphorylation of occludin in the isolated brain capillaries PMID: 19319148
    38. Occludin performs a structural role in inhibiting paracellular diffusion, and a signaling role involving interactions of dimeric and monomeric occludin isoforms within plasma membrane lipid raft domains. PMID: 19457074
    39. Fg binding to endothelial cells (ECs) alters expression of actin-associated endothelial tight junction proteins. PMID: 19507189

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, tight junction.
  • 蛋白家族：
    ELL/occludin family
  • 数据库链接：

    KEGG: rno:83497

    STRING: 10116.ENSRNOP00000024674

    UniGene: Rn.31429