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  4. Recombinant Rat Potassium voltage-gated channel subfamily A member 2 (Kcna2)

Recombinant Rat Potassium voltage-gated channel subfamily A member 2 (Kcna2)

  • 货号：
    CSB-CF012007RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Kcna2
  • Uniprot No.：
  • 别名：
    Kcna2; Potassium voltage-gated channel subfamily A member 2; RAK; RBK2; RCK5; Voltage-gated potassium channel subunit Kv1.2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-499
  • 氨基酸序列
    MTVATGDPVDEAAALPGHPQDTYDPEADHECCERVVINISGLRFETQLKTLAQFPETLLG DPKKRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRRPVNVPLDIFSEEIRFYELGE EAMEMFREDEGYIKEEERPLPENEFQRQVWLLFEYPESSGPARIIAIVSVMVILISIVSF CLETLPIFRDENEDMHGGGVTFHTYSNSTIGYQQSTSFTDPFFIVETLCIIWFSFEFLVR FFACPSKAGFFTNIMNIIDIVAIIPYFITLGTELAEKPEDAQQGQQAMSLAILRVIRLVR VFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADERDSQFPS IPDAFWWAVVSMTTVGYGDMVPTTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRE TEGEEQAQYLQVTSCPKIPSSPDLKKSRSASTISKSDYMEIQEGVNNSNEDFREENLKTA NCTLANTNYVNITKMLTDV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and the central nervous system, but also in the cardiovascular system. Prevents aberrant action potential firing and regulates neuronal output. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNA7, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel. Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation of delayed rectifier potassium channels. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to a particular potassium channel family member. Homotetrameric KCNA2 forms a delayed-rectifier potassium channel that opens in response to membrane depolarization, followed by slow spontaneous channel closure. In contrast, a heteromultimer formed by KCNA2 and KCNA4 shows rapid inactivation. Response to toxins that are selective for KCNA1, respectively for KCNA2, suggests that heteromeric potassium channels composed of both KCNA1 and KCNA2 play a role in pacemaking and regulate the output of deep cerebellar nuclear neurons. KCNA2-containing channels play a presynaptic role and prevent hyperexcitability and aberrant action potential firing. Response to toxins that are selective for KCNA2-containing potassium channels suggests that in Purkinje cells, dendritic subthreshold KCNA2-containing potassium channels prevent random spontaneous calcium spikes, suppressing dendritic hyperexcitability without hindering the generation of somatic action potentials, and thereby play an important role in motor coordination. Plays a role in the induction of long-term potentiation of neuron excitability in the CA3 layer of the hippocampus. May function as down-stream effector for G protein-coupled receptors and inhibit GABAergic inputs to basolateral amygdala neurons. May contribute to the regulation of neurotransmitter release, such as gamma-aminobutyric acid (GABA). Contributes to the regulation of the axonal release of the neurotransmitter dopamine. Reduced KCNA2 expression plays a role in the perception of neuropathic pain after peripheral nerve injury, but not acute pain. Plays a role in the regulation of the time spent in non-rapid eye movement (NREM) sleep.
  • 基因功能参考文献：
    1. indings suggest that increased dorsal horn DNMT3a contributes to bone cancer pain through silencing dorsal horn Kv1.2 expression PMID: 29056068
    2. Ventricular Kcna2 AS expression increases in rats with CHF and contributes to reduced IKs, prolonged APs, and the occurrence of ventricular arrhythmias by silencing Kcna2. PMID: 29263036
    3. Data indicate that in cryo-electron microscopy (cryo-EM) images of the relatively small Kv1.2 channel complex, the protein particle's contribution is quite weak compared to the membrane density. PMID: 26835990
    4. the dynamics of the ion conduction processes in the Kv1.2 pore domain PMID: 26950215
    5. Kv1.2 mediates heterosynaptic modulation of direct cortical synaptic inputs in CA3 pyramidal cells PMID: 26047212
    6. An R300C mutation creates a large cavity in the voltage sensing domain. PMID: 25588216
    7. Data suggest that exercise training reverses the pathological expression of the Kv1.2, Kv1.5, and BKCa channels in aortic myocytes from spontaneously hypertensive rats. PMID: 25661478
    8. a majority of dorsal root ganglion (DRG) neurons were positive for Kv channel alpha subunit Kv1.2. PMID: 24472174
    9. The alpha subunit of Kv1.2 channels determines the pharmacological characteristics of potassium channel blockers by their position in the channel. PMID: 23725331
    10. Cell-surface Kv1.2 alpha-subunit levels are controlled by secretin-regulated Kv1.2 endocytic trafficking. PMID: 22764231
    11. myelin regulates both trafficking and activity of Kv1 channels along hippocampal axons through TAG-1 PMID: 21602278
    12. Kv1.1 or 1.2 homomers and their concatenated forms between the pairs of adjacently and diagonally arranged heterotetramers show differential sensitivity to tetraethylammonium. PMID: 20805574
    13. proper amounts of Kv1 channels and their associated proteins are required for efficient transport of Kv1 channel proteins along axons PMID: 20694152
    14. Results indicate that the extreme C-terminal end of the S6 inner helix plays an important regulatory role in the activation of the C-terminal-truncated Kv1.2 channel. PMID: 19947938
    15. analysis of full-length Shaker potassium channel Kv1.2 structure by normal-mode-based X-ray crystallographic refinement PMID: 20534430
    16. atomic-resolution observations of permeation and gating in a K(+) channel, based on molecular dynamics simulations of the Kv1.2 pore domain PMID: 20231479
    17. Kv1.2 channels have general presynaptic function in suppressing terminal hyperexcitability during depolarising after-potential PMID: 12777451
    18. crystal structure, at a resolution of 2.9 angstroms PMID: 16002581
    19. Kv 1.2 was found in cochlear nucleus neuronal cell bodies at birth and postnatal day 21 through adulthood, labeling for potassium channel was in axonal processes, whereas the no cell bodies labeled for Kv 1.2. PMID: 16122713
    20. study shows that activation of presynaptic mu opioid receptors primarily attenuates GABAergic synaptic inputs to central nucleus of the amygdala-projecting neurons in the basolateral amygdala through a signaling mechanism involving Kv1.1 & Kv1.2 channels PMID: 16306173
    21. Results suggest that amino acids in the pore region help regulate ion permeability or cellular trafficking by affecting glycosylation of Kv1.2. PMID: 16770729
    22. Enhanced nitrosative stress in diabetes mellitus contributes to Kcna2 channel dysfunction in the coronary microcirculation, which may be restored by ebselen. PMID: 17675568
    23. Data show that in the KcsA potassium channel, the number and strength of hydrogen bonds between residues in the selectivity filter and pore helix determine the rate and extent of C-type inactivation, which can also be engineered in Kv1.2 channels. PMID: 17922012
    24. impairment of cAMP-mediated endothelium independent vasodilation of rat small coronary artery by STZ-induced diabetes was resulted from decrease of mRNA and protein expressions of Kv channels, and which leads to a reduced current from Kv channels PMID: 17982915
    25. cluster of cytoplasmic C-terminal phosphorylation sites regulates Kv1.2 trafficking PMID: 18056633
    26. Expression of Kv1.2, Kv1.5, and Kv2.1 was higher in mesenteric arteries but was not different in aortas of SHR rats as compared to WKY rats PMID: 18174882
    27. R294H, A351H mutagenesis and also in combination with D352G, E353S mutations (oocytes expression)show intersubunit histidine metallic bridges formed between the first arginine of S4 (R294) and residues A351 or D352 of the pore domain PMID: 18504314
    28. We conclude that Kv1.2 in microglia modulates IL-1beta and TNF-alpha expression and ROS production probably by regulating the intracellular potassium concentration. PMID: 18627436
    29. The Kv1.2 channel exhibits functional properties that are distinct from Kv1.2 channels reported in the literature. PMID: 18638484
    30. the dynamic interaction of the C-terminus with the S4-S5 linker from a neighboring subunit of the Kv1.2 channel provides a mechanism for its C-terminus to regulate the channel activation PMID: 19247844

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Membrane. Cell projection, axon. Cell junction, synapse. Cell junction, synapse, synaptosome. Cell junction, synapse, presynaptic cell membrane. Cell projection, dendrite. Endoplasmic reticulum membrane. Cell projection, lamellipodium membrane. Endosome. Perikaryon. Cell junction, paranodal septate junction.
  • 蛋白家族：
    Potassium channel family, A (Shaker) (TC 1.A.1.2) subfamily, Kv1.2/KCNA2 sub-subfamily
  • 组织特异性：
    Detected in neurons in dorsal root ganglion. Detected in hippocampus neurons. Detected on neurons of the anteroventral cochlear nucleus. Detected in renal arteries. Detected in neurons of the medial nucleus of the trapezoid body. Detected in neurons in th
  • 数据库链接：

    KEGG: rno:25468

    STRING: 10116.ENSRNOP00000042653

    UniGene: Rn.10298