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  4. Recombinant Rat Potassium voltage-gated channel subfamily A member 4 (Kcna4)

Recombinant Rat Potassium voltage-gated channel subfamily A member 4 (Kcna4)

  • 中文名称：
    大鼠Kcna4重组蛋白
  • 货号：
    CSB-CF012009RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Kcna4
  • Uniprot No.：
  • 别名：
    Kcna4; Potassium voltage-gated channel subfamily A member 4; RCK4; RHK1; RK3; Voltage-gated potassium channel subunit Kv1.4
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-655
  • 氨基酸序列
    MEVAMVSAESSGCNSHMPYGYAAQARARERERLAHSRAAAAAAVAAATAAVEGTGGSGGG PHHHHQTRGAYSSHDPQGSRGSREEEATRTEKKKKLHHRQSSFPHCSDLMPSGSEEKILR ELSEEEEDEEEEEEEEEEGRFYYSEEDHGDGCSYTDLLPQDDGGGGGYSSVRYSDCCERV VINVSGLRFETQMKTLAQFPETLLGDPEKRTQYFDPLRNEYFFDRNRPSFDAILYYYQSG GRLKRPVNVPFDIFTEEVKFYQLGEEALLKFREDEGFVREEEDRALPENEFKKQIWLLFE YPESSSPARGIAIVSVLVILISIVIFCLETLPEFRDDRDLIMALSAGGHSRLLNDTSAPH LENSGHTIFNDPFFIVETVCIVWFSFEFVVRCFACPSQALFFKNIMNIIDIVSILPYFIT LGTDLAQQQGGGNGQQQQAMSFAILRIIRLVRVFRIFKLSRHSKGLQILGHTLRASMREL GLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYGDMKPITVGGK IVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETENEEQTQLTQNAVSCPYLPSNLLKKFR SSTSSSLGDKSEYLEMEEGVKESLCGKEEKCQGKGDDSETDKNNCSNAKAVETDV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel. Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Homotetrameric KCNA4 forms a potassium channel that opens in response to membrane depolarization, followed by rapid spontaneous channel closure. Likewise, a heterotetrameric channel formed by KCNA1 and KCNA4 shows rapid inactivation.
  • 基因功能参考文献：
    1. Four prolines and four lysines in a Kv1.4 homotetramer might provide a binding site for a putative endoplasmic reticulum-export molecule to ensure high cell surface protein expression of the channel. PMID: 28054303
    2. synaptotagmin I is an interacting protein of Kv1.4 channel. PMID: 24423395
    3. Kv1.4 K(+) channels are potently regulated by intracellular free heme; heme binds to the N-terminal inactivation domain and thereby impairs the inactivation process PMID: 24082096
    4. Endogenous H2S generating enzyme cystathionine-beta-synthetase was co-localized well with Kv1.1 and Kv1.4 in trigeminal ganglion neurons. PMID: 23413915
    5. Immunocytochemistry indicated that protein expression for Kv1.4 increased between 1 wk and 4-5 wk of age. PMID: 21451062
    6. N-type inactivation prevention (NIP) domain function was shown to require positioning of Kv1.6 adjacent to the Kv1.4 subunit. PMID: 21352098
    7. NMR assignments for 48 kDa tetramer of the T1 domain of Kv1.4 [Kv1.4] PMID: 19888682
    8. Kv1.4 channels are widely distributed in the rat brain and activation of this channel would have different modulatory effects on neuronal excitability. PMID: 14615029
    9. preventing N-glycosylation of Kv1.4 decreased its protein stability, induced its high partial intracellular retention, and decreased its cell surface protein levels PMID: 14688283
    10. Kv3.4 subunit immunoreactivity (Kv3.4-IR) was widespread, with dense, punctate staining in many regions including the intermediolateral cell column (IML) and the dorsal vagal nucleus (DVN), nucleus ambiguus (NA) and nucleus tractus solitarius (NTS). PMID: 15207333
    11. These results suggest that diffusible factors coordinate the pregnancy-associated changes in molecular compositions of myometrial Kv4-KChIP channel complexes. PMID: 15454398
    12. Immunohistochemical co-expression of carbonic anhydrase II with Kv1.4 and TRPV1 in rat small-diameter trigeminal ganglion neurons. PMID: 15885224
    13. Taken together, elevation of the neuronal transmission stimulates the phosphorylation of Kv1.4 at Ser229 via the Ca2+ influx through NMDA receptor. PMID: 16000151
    14. these results demonstrate that Kv4alpha subunits encode IA channels and that IA plays a pivotal role in shaping the waveforms of individual action potentials and in controlling repetitive firing in visual cortical pyramidal neurons. PMID: 16207878
    15. Continuum electrostatics was used to investigate the interactions of Kv1.4 mutants H508 or K532 with K(+) sites in the pore. PMID: 17359997
    16. Temporomandibular joint(TMJ) inflammation reduces expression of Kv1.4 subunits in small-/medium sized (Adelta-/C-) trigeminal ganglion neurons and this may contribute to trigeminal inflammatory allodynia in TMJ disorder. PMID: 17584507
    17. Removal of the fast N-type inactivation gate, delta (6-46), reveals a steric interference of internal TEA with an internally located slow inactivation gate in Xenopus oocytes expression system. PMID: 19029372
    18. Bladder inflammation increases bladder afferent neuron excitability by decreasing expression of Kv1.4 alpha-subunits. PMID: 19279288
    19. Both arachidonic acid and Trolox potently modulate Kv1.4 and Kv4.2 channel alpha-subunits, thereby presumably tuning presynaptic transmitter release and postsynaptic somatodendritic excitability in synaptic transmission and plasticity. PMID: 19453640

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell projection, axon.
  • 蛋白家族：
    Potassium channel family, A (Shaker) (TC 1.A.1.2) subfamily, Kv1.4/KCNA4 sub-subfamily
  • 组织特异性：
    Detected in brain (at protein level). Heart and brain.
  • 数据库链接：

    KEGG: rno:25469

    STRING: 10116.ENSRNOP00000006524

    UniGene: Rn.9884