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  4. Recombinant Rat Rhodopsin (Rho)

Recombinant Rat Rhodopsin (Rho)

  • 货号：
    CSB-CF019681RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Rho; Rhodopsin
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-348
  • 氨基酸序列
    MNGTEGPNFYVPFSNITGVVRSPFEQPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY VTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLG GEIGLWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIP EGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIVIFFCYGQLVFTVKEAAAQQQES ATTQKAEKEVTRMVIIMVIFFLICWLPYASVAMYIFTHQGSNFGPIFMTLPAFFAKTASI YNPIIYIMMNKQFRNCMLTSLCCGKNPLGDDEASATASKTETSQVAPA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.
  • 基因功能参考文献：
    1. these results suggest that retinoid metabolism in the eye is impaired in type 1 diabetes, which leads to deficient generation of visual pigments and neural retinal dysfunction in early diabetes. PMID: 28734946
    2. EGF induces Ca(2+) sensitization in vascular smooth muscle by Rho-kinase-dependent inactivation of MLCP mediated by the EGF receptor/MEK/Erk1/2 pathway PMID: 26004531
    3. Allele-specific ASO-mediated knockdown of mutant P23H rhodopsin expression slowed the rate of photoreceptor degeneration and preserved the function of photoreceptor cells in eyes of the P23H rhodopsin transgenic rat. PMID: 26436889
    4. Phosphorylation of the Rho/ROCK signaling target cofilin. PMID: 25108566
    5. We examine and compare the contribution of endoplasmic reticulum stress to retinal degeneration in several vertebrate models of retinitis pigementosa generated through expression of mutant rhodopsins. PMID: 24664747
    6. Rho kinase is involved in endothelial dysfunction in type 1 diabetes. PMID: 24129169
    7. The aim of this study was to examine the onset of Unfolded Protein Response (UPR) gene expression in S334ter Rho retinas to determine if UPR is activated in autosomal dominant retinitis pigmentosa animal models. PMID: 22432009
    8. Strong activation of calpain and poly(ADP-ribose) polymerase (PARP) was found in rhodopsin mutants. PMID: 21765948
    9. Phosphorylation of rhodopsin during dark adaptation in an animal model of retinitis pigmentosa. PMID: 12965217
    10. rats homozygous for P23H rhodopsin mutations have severe loss of rod function already seen by postnatal day 21 PMID: 15911114
    11. Insulin receptor phosphorylation in rod photoreceptors is signaled through the G-protein-coupled receptor rhodopsin. PMID: 17272282
    12. Experiments with dissociated hippocampal cells show that the main factor limiting the spatial resolution is Rhodopsin current density rather than scattering of the excitation light. PMID: 18654856

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Opsin subfamily
  • 数据库链接：

    KEGG: rno:24717

    STRING: 10116.ENSRNOP00000061473

    UniGene: Rn.92530