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  4. Recombinant Rat Synaptotagmin-4 (Syt4)

Recombinant Rat Synaptotagmin-4 (Syt4)

  • 中文名称：
    大鼠Syt4重组蛋白
  • 货号：
    CSB-CF023040RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Syt4
  • Uniprot No.：
  • 别名：
    Syt4; Synaptotagmin-4; Synaptotagmin IV; SytIV
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-425
  • 氨基酸序列
    MAPITTSRVEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRRSAKSNKTPPYKFVHVLKGVDIYPENLSSKKKFGGDDKSEAKRKAALPNLSLHLDLEKRDLNGNFPKTNPKAGSSSDLENVTPKLFPETEKEAVSPESLKSSTSLTSEEKQEKLGTLFLSLEYNFEKKAFVVNIKEAQGLPAMDEQSMTSDPYIKMTILPEKKHKVKTRVLRKTLDPVFDETFTFYGVPYPHIQELSLHFTVLSFDRFSRDDVIGEVLVPLSGIELSDGKMLMTREIIKRNAKKSSGRGELLVSLCYQSTTNTLTVVVLKARHLPKSDVSGLSDPYVKVNLYHAKKRISKKKTHVKKCTPNAVFNELFVFDIPCESLEEISVEFLVLDSERGSRNEVIGRLVLGATAEGSGGGHWKEICDFPRRQIAKWHMLCDG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Synaptotagmin family member which does not bind Ca(2+). Involved in neuronal dense core vesicles (DCVs) mobility through its interaction with KIF1A. Upon increased neuronal activity, phosphorylation by MAPK8/JNK1 destabilizes the interaction with KIF1A and captures DCVs to synapses. Plays a role in dendrite formation by melanocytes.
  • 基因功能参考文献：
    1. neuronal activity increased dense core vesicle capture via JNK-dependent phosphorylation of the S135 site of Synaptotagmin-4 PMID: 29166604
    2. The increased expression of Synaptotagmin IV in optic nerve crush eyes displays the possible neuroprotective effect of bone marrow mononuclear cell treatment PMID: 22695963
    3. Immunocytochemistry in rat chromaffin cells shows differential distribution of synaptotagmin-1, -4, -7, and -9. PMID: 21287204
    4. These data support an inhibitory effect of syt IV on release of vesicles and their transmitter content PMID: 20735850
    5. Rat and Drosophila synaptotagmin 4 have opposite effects during SNARE-catalyzed membrane fusion. PMID: 20688915
    6. The effects of syt IV on fusion pores are discussed in terms of structural models and kinetic mechanisms. PMID: 20303854
    7. present several lines of evidence showing that the Syt IV is normally localized in the Golgi or immature vesicles at the cell periphery and is sorted to fusion-competent mature dense-core vesicles in response to short nerve growth factor stimulation PMID: 12446703
    8. NMR analysis of Ca2+ binding to synaptotagmin 4 PMID: 15311271
    9. synaptotagmin IV protein trafficking from the Golgi to the cell periphery is regulated by forskolin PMID: 15496596
    10. Cells with overexpressed rat SytIV were unaltered in basal release probability, Ca2+ dependence of release, short-term plasticity, & fusion pore kinetics. Mutant Syt I (less Ca2+ affinity) reduced release probability & altered Ca2+ dependence of release. PMID: 16407532
    11. Downregulation of Syt IV reduced Ca(2+)-triggered exocytosis of dense core vesicles. Syt I silencing, however, had no effect on vesicular release. PMID: 18468511
    12. Given the neuroendocrine functions of the posterior pituitary, changes in Syt IV levels could be involved in endocrine transitions involving alterations in the release of the neuropeptides oxytocin and vasopressin PMID: 19136969
    13. Expression of Syt4 increases 9-fold at night in the rat pineal gland; treatment with dibutyryl cyclic AMP increases expresssion. PMID: 19103603

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle membrane; Single-pass membrane protein.
  • 蛋白家族：
    Synaptotagmin family
  • 组织特异性：
    Widely expressed. Expressed in the brain. Expressed in pituitary gland, cerebellum, cortex, hypothalamus and hippocampus.
  • 数据库链接：

    KEGG: rno:64440

    STRING: 10116.ENSRNOP00000023593

    UniGene: Rn.126675