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  4. Recombinant Rat Transient receptor potential cation channel subfamily V member 6 (Trpv6)

Recombinant Rat Transient receptor potential cation channel subfamily V member 6 (Trpv6)

  • 中文名称：
    Recombinant Rat Transient receptor potential cation channel subfamily V member 6 (Trpv6)
  • 货号：
    CSB-CF870815RA
  • 规格：
    ￥12960
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
  • Uniprot No.：
  • 别名：
    TrpV6;Calcium transport protein 1;CaT1;Epithelial calcium channel 2;ECaC2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    88.9 kDa
  • 表达区域：
    1-767aa
  • 氨基酸序列
    MGPLQREGRPALGDANVAPGSSPGGVWHQPQPPKDSAFHPMGWSLPKEKGLILCLWNKFCRWFHRRESWAQSRDEQNLLQQKRIWESPLLLAAKENNVQALIKLLKFEGCEVHQKGAMGETALHIAALYDNLEAAMVLMEAAPELVFEPMTSELYEGQTALHIAVINQNVNLVRALLARGASVSARATGSVFHYRPHNLIYYGEHPLSFAACVGSEEIVRLLIEHGADIRAQDSLGNTVLHILILQPNKTFACQMYNLLLSYDGGDHLKSLELVPNNQGLTPFKLAGVEGNIVMFQHLMQKRKHIQWTYGPLTSTLYDLTEIDSSGDDQSLLELIVTTKKREARQILDQTPVKELVSLKWKRYGRPYFCVLGAIYVLYIICFTMCCVYRPLKPRITNRTNPRDNTLLQQKLLQEAYVTPKDDLRLVGELVSIVGAVIILLVEIPDIFRLGVTRFFGQTILGGPFHVIIVTYAFMVLVTMVMRLTNSDGEVVPMSFALVLGWCNVMYFARGFQMLGPFTIMIQKMIFGDLMRFCWLMAVVILGFASAFYIIFQTEDPDELGHFYDYPMALFSTFELFLTIIDGPANYDVDLPFMYSITYAAFAIIATLLMLNLLIAMMGDTHWRVAHERDELWRAQVVATTVMLERKLPRCLWPRSGICGREYGLGDRWFLRVEDRQDLNRQRIRRYAQAFQQQDDLYSEDLEKDSGEKLEMARPFGAYLSFPTPSVSRSTSRSSTNWDRLRQGALRKDLQGIINRGLEDGEGWEYQI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium selective cation channel that mediates Ca(2+) uptake in various tissues, including the intestine. Important for normal Ca(2+) ion homeostasis in the body, including bone and skin. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.
  • 基因功能参考文献：
    1. we present a model for TRPV6 CaM-dependent inactivation, which involves a novel so-called "two-tail" mechanism whereby CaM bridges two TRPV6 monomers resulting in closure of the channel pore. PMID: 29505720
    2. TRPV6 could regulate certain chondrocyte functions, including ECM secretion, cell proliferation, and apoptosis I osteoarthritis. PMID: 28063212
    3. crystal structure of rat TRPV6 at 3.25 A resolution PMID: 27296226
    4. study demonstrates for the first time the expression of TRPV6 in INS-1E cells and rat pancreatic beta cells and describes its role in modulating calcium signaling, beta cell proliferation and insulin mRNA expression PMID: 26384871
    5. regulatory component that controls activation of both CaT1 and CRAC (Ca(2+) release-activated Ca(2+) channel) channels. PMID: 12011062
    6. role of TRPV6 as calcium sensor in rat and human cells PMID: 12138163
    7. TRPV5 and TRPV6 are regulated by 17beta-E2 and 1,25(OH)2D3, whereas dietary Ca2+ is positively involved in the regulation of TRPV6 only. PMID: 12620887
    8. the TRPV6 channel is regulated by calcium PMID: 15184369
    9. Together, these results suggest that CaT1 is regulated by P4 at diestrus via a PR-dependent pathway. PMID: 16825604
    10. The influence of NMDA receptor activation on mammalian target of rapamycin (mTOR) activity and neuronal process extension is mediated by CAT1. PMID: 17234578
    11. Regulation of TRPV6 gene expression by short chain fatty acids may be a molecular mechanism involved in the promotion of calcium absorption by fructooligosaccharides in rats. PMID: 19056662
    12. TRPV6 gene may function as a key element in controlling calcium transport in the uterus between the embryo and the placenta during pregnancy PMID: 19457270

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Transient receptor (TC 1.A.4) family, TrpV subfamily, TRPV6 sub-subfamily
  • 组织特异性：
    Expressed in duodenum, proximal jejunum, cecum, and colon.
  • 数据库链接：

    KEGG: rno:114246

    STRING: 10116.ENSRNOP00000020616

    UniGene: Rn.205943