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  4. Recombinant Rhizobium meliloti C4-dicarboxylate transport sensor protein dctB (dctB)

Recombinant Rhizobium meliloti C4-dicarboxylate transport sensor protein dctB (dctB)

  • 中文名称：
    Recombinant Rhizobium meliloti C4-dicarboxylate transport sensor protein dctB(dctB)
  • 货号：
    CSB-CF318394RKU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    dctB
  • Uniprot No.：
  • 别名：
    dctB; RB1524; SMb20612; C4-dicarboxylate transport sensor protein DctB
  • 种属：
    Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-621
  • 氨基酸序列
    MHHVRMVKLPAEASDPHALRSRARRSWLVFAAVALVLLAAGLLLARDYGRSQALAGLAGQ SRIDASLKASLLRAVVERQRALPLVLADDAAIRGALLSPDRPSLDRINRKLEALATSAEA AVIYLIDRSGVAVAASNWQEPTSFVGNDYAFRDYFRLAVRDGMAEHFAMGTVSNRPGLYI SRRVDGPGGPLGVIVAKLEFDGVEADWQASGKPAYVTDRRGIVLITSLPSWRFMTTKPIA EDRLAPIRESLQFGDAPLLPLPFRKIEARPDGSSTLDALLPGDSTAAFLRVETMVPSTNW RLEQLSPLKAPLAAGAREAQLLTLAALVPLLALAALLLRRRQVVAMRSAEERLARNALEA SVEERTRDLRMARDRLETEIADHRQTTEKLQAVQQDLVQANRLAILGQVAAGVAHEINQP VATIRAYADNARTFLHRGQTVTAAENMESIAELTERVGAITDELRRFARKGHFAAGPTAM KEVVEGALMLLRSRFAGRMDAIRLDLPPDGLQALGNRIRLEQVLINLLQNALEAIGDSED GAIQVRCEEAAGGIALTVADNGPGIAADVREELFTPFNTSKEDGLGLGLAISKEIVSDYG GTIEVESGPSGTTFAVNLKKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Member of the two-component regulatory system DctB/DctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates DctD in response to environmental signals.
  • 基因功能参考文献：
    1. These results suggest that DctBp's signal perception and transduction occur through a 'ligand-induced dimeric switch', in which the changes in the dimeric conformations upon ligand binding are responsible for the signal transduction in DctB. PMID: 20149110
    2. The crystals diffracted to 2.3 A resolution and belonged to P42 space group with unit cell dimensions of a = b = 71.77 A, c = 227.14 A. The asymmetric unit contains four molecules with a corresponding VM of 2.4 A3 Da(-1) and a solvent content of 49.1%. PMID: 16332458
    3. structures of the different ligand binding states revealed conformational changes initiated upon ligand binding and propagated to the N-terminal domain responsible for dimerization, providing insights into the mechanism of signal transduction PMID: 18725229
  • 亚细胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 数据库链接：

    KEGG: sme:SM_b20612