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  4. Recombinant Saccharomyces cerevisiae 3-hydroxyacyl-CoA dehydratase PHS1 (PHS1)

Recombinant Saccharomyces cerevisiae 3-hydroxyacyl-CoA dehydratase PHS1 (PHS1)

  • 中文名称：
    酿酒酵母PHS1重组蛋白
  • 货号：
    CSB-CF328181SVG
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    PHS1
  • Uniprot No.：
  • 别名：
    PHS1; YJL097W; J0902; Very-long-chain; 3R-3-hydroxyacyl-CoA dehydratase PHS1; 3-hydroxyacyl-CoA dehydratase PHS1; HACD; PTPLA homolog involved in sphingolipid biosynthesis protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-217
  • 氨基酸序列
    MSKKLASPLSFLPLYNLLSAVGWSYLLYLVISLYPKVGQPAFFYQTKNVATLVQCGAIIE IINSFLGVVRSPLLTTVAQVSSRLLVVLGIFQLLPNTSGVQSVVYISLLLAWSITEIVRY LYYFFMLVFKNGAPKILILLRYNLFWILYPTGVASELRIIYCALNAAESQYSLLYKRILI AAMLAYIPGFPMLFLHMVAQRKKVMKSLRSSFGKKLI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.
  • 基因功能参考文献：
    1. The enzyme kinetics study implicated the direct involvement of the Arg83 and Gly152 residues in the catalytic process. PMID: 23416297
    2. Phs1 is a 3-hydroxyacyl-CoA dehydratase involved in very long-chain fatty acid elongation PMID: 18272525
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Very long-chain fatty acids dehydratase HACD family
  • 数据库链接：

    KEGG: sce:YJL097W

    STRING: 4932.YJL097W