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  4. Recombinant Saccharomyces cerevisiae Non-homologous end-joining protein 1 (NEJ1)

Recombinant Saccharomyces cerevisiae Non-homologous end-joining protein 1 (NEJ1)

  • 货号：
    CSB-CF313267SVG
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    NEJ1
  • Uniprot No.：
  • 别名：
    NEJ1; LIF2; YLR265C; Non-homologous end-joining protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-342
  • 氨基酸序列
    MDSELKGQQLSDAEWCVKKINGEGNCLLLFLPMSSPTTIVMIVLVSLERLVPYVFKLSQT QLSQQCQSQGFTDSISLNLIKLKLMDILQAPQEINQIGLVDSNLVFSFDVSADITVSINS VPSHVTKDMFYMILQSLCMLLLKLVNLSTQYHYVQRDILNEKQKCLDFLLISLRDLDGGS KVISQWAPENSKNYESLQQCTDDDIIKKLLHKGKFQHQEFLADSLKTLLSLRNKFQDVSR FEESGELNKKERVRFPAVNHFYNDDFELQADPTNEARPNSRGKIKPKTDFKPKSRESSTS SQLRLENFSESEATPEKTKSSSSLVEEYPQKKRKFGKVRIKN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in non-homologous end joining (NHEJ). Facilitates the transport of LIF1 into the nucleus, where it can interact with DNA ligase DNL4 to repair double-strand breaks (DSB). Mediates mating-type regulation of NHEJ. Prevents chromosome circularisation by NHEJ in absence of telomerase.
  • 基因功能参考文献：
    1. Our results reveal novel roles for Nej1 in the recruitment of Pol4 and Rad27 to in vivo DNA double strand breaks and the coordination of the end processing and ligation reactions of non-homologous end joining. PMID: 25942368
    2. C-terminus of Nej1 is critical for nuclear localization and non-homologous end-joining PMID: 24369855
    3. Nej1 not only plays an important role in determining repair pathway choice by participating in the initial NHEJ complex formed at DSBs but also contributes to the reactivation of Dnl4-Lif1 after repair is complete PMID: 21149442
    4. Nej1p is phosphorylated in response to DNA damage in a manner that relies on the DNA damage checkpoint kinases Mec1p, Rad53p and Dun1p. PMID: 17101301
    5. Nej1 interacts with Lif1 and supports NHEJ, similar to the distantly related mammalian Nej1 orthologue XLF PMID: 17567543
    6. Structural characterization reveals that Nej1p exists as a dimer, and that residues 1-244 are sufficient for dimer formation PMID: 17765666
    7. Nej1 physically interacts with Srs2. PMID: 19571008

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  • 亚细胞定位：
    Cytoplasm. Nucleus membrane; Multi-pass membrane protein.
  • 蛋白家族：
    XLF family
  • 数据库链接：

    KEGG: sce:YLR265C

    STRING: 4932.YLR265C