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Recombinant Saccharomyces cerevisiae Palmitoyltransferase PFA3 (PFA3)

  • 中文名称:
    酿酒酵母PFA3重组蛋白
  • 货号:
    CSB-CF337234SVG
  • 规格:
  • 来源:
    in vitro E.coli expression system
  • 其他:

产品详情

  • 基因名:
    PFA3
  • Uniprot No.:
  • 别名:
    PFA3; YNL326C; N0325; Palmitoyltransferase PFA3; Protein fatty acyltransferase 3
  • 种属:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度:
    full length protein
  • 表达区域:
    1-336
  • 氨基酸序列
    MNDRLSLTSLFPRCLTTCLYIWTAYITLTRIHQIPRWFLALTIVPTLAVALYTYYKVIAR GPGSPLDFPDLLVHDLKAAENGLELPPEYMSKRCLTLKHDGRFRVCQVCHVWKPDRCHHC SSCDVCILKMDHHCPWFAECTGFRNQKFFIQFLMYTTLYAFLVLIYTCYELGTWFNSGSF NRELIDFHLLGVALLAVAVFISVLAFTCFSIYQVCKNQTTIEVHGMRRYRRDLEILNDSY GTNEHLENIFDLGSSMANWQDIMGTSWLEWILPIETFKYKKSKHTKDEKGLYFNVRPQVQ DRLLSSRCLEDQLLRRVTPRPSLEADRASVEIIDAN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签:
    N-terminal 10xHis-tagged
  • 产品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization.
  • 基因功能参考文献:
    1. This study focusses on the function of PFA3 as the third member of the DHHC-CRD family found to have protein acyltransferase activity. PMID: 16186255
    2. The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8. PMID: 16301533
    3. Data show that for Pfa3 and its substrate Vac8, there are two consecutive steps in the acylation reaction: initial binding independent of cysteine in the DHHC box, but requiring myristoylation of Vac8, and a DHHC-motif dependent acylation. PMID: 19453970
  • 亚细胞定位:
    Vacuole membrane; Multi-pass membrane protein.
  • 蛋白家族:
    DHHC palmitoyltransferase family, PFA3 subfamily
  • 数据库链接:

    KEGG: sce:YNL326C

    STRING: 4932.YNL326C