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  4. Recombinant Saccharomyces cerevisiae Protein ROT1 (ROT1)

Recombinant Saccharomyces cerevisiae Protein ROT1 (ROT1)

  • 中文名称：
    酿酒酵母ROT1重组蛋白
  • 货号：
    CSB-CF311811SVG
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    ROT1
  • Uniprot No.：
  • 别名：
    ROT1; YMR200W; YM8325.01; Protein ROT1; Reversal of TOR2 lethality protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-256
  • 氨基酸序列
    EDESNSIYGTWSSKSNQVFTGPGFYDPVDELLIEPSLPGLSYSFTEDGWYEEATYQVSGN PRNPTCPMASLIYQHGTYNISENGTLVLNPIEVDGRQLFSDPCNDDGVSTYSRYNQTETF KEYAVGIDPYHGIYTLQLYQYDGTPMQPLYLAYRPPMMLPTETLNPTSSATSTDDPSSNK KRSLRSLVRRSLENRHKTNAIKRQNTSFLTSNAIWYISAGMLGVGSLLFLAF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies. Controls actin cytoskeleton polarization to the mother-bud neck and CLB2 protein stability.
  • 基因功能参考文献：
    1. Rot1 needs the transmembrane Ser250 to interact with other membrane components and to exert its functional role. PMID: 24303792
    2. the role of Rot1 as a chaperone required to ensure proper glycosylation is demonstrated in yeast PMID: 22492205
    3. The results show that two regions, amino acids 26-60 and 200-228, are involved in the post-translational translocation of Rot1 across the endoplasmic reticulum membrane. PMID: 20608986
    4. Rot1p participates in protein folding with Kar2p. PMID: 16567426
    5. study revealed a role for Rot1 in cell morphogenesis during cell cycle progression; functional antagonism between Rot1 and Clb2 is supported by genetic and biochemical data, which indicate that Rot1 is implicated in Clb2 protein degradation PMID: 17606994
    6. Characterization of the post-translational modification, transport and membrane topology of Rot1 is reported. PMID: 17914748
    7. is an essential molecular chaperone in the endoplasmic reticulum PMID: 18508919

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    ROT1 family
  • 数据库链接：

    KEGG: sce:YMR200W

    STRING: 4932.YMR200W