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重组蛋白

产品名称 货号 规格
Recombinant Lysinibacillus sphaericus Pseudouridine-5'-phosphate glycosidase (psuG) CSB-YP544827LRV
CSB-EP544827LRV
CSB-BP544827LRV
CSB-MP544827LRV
CSB-EP544827LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP544828DUJ
CSB-EP544828DUJ
CSB-BP544828DUJ
CSB-MP544828DUJ
CSB-EP544828DUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP544829ENP
CSB-EP544829ENP
CSB-BP544829ENP
CSB-MP544829ENP
CSB-EP544829ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Endonuclease V (nfi) CSB-YP544830ENP
CSB-EP544830ENP
CSB-BP544830ENP
CSB-MP544830ENP
CSB-EP544830ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP544831ENP
CSB-EP544831ENP
CSB-BP544831ENP
CSB-MP544831ENP
CSB-EP544831ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2- (5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase CSB-YP544832ENP
CSB-EP544832ENP
CSB-BP544832ENP
CSB-MP544832ENP
CSB-EP544832ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis serovar L2 Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP544833DSG
CSB-EP544833DSG
CSB-BP544833DSG
CSB-MP544833DSG
CSB-EP544833DSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis serovar L2 N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP544834DSG
CSB-EP544834DSG
CSB-BP544834DSG
CSB-MP544834DSG
CSB-EP544834DSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia rickettsii Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP544835RNB
CSB-EP544835RNB
CSB-BP544835RNB
CSB-MP544835RNB
CSB-EP544835RNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP544836AVV
CSB-EP544836AVV
CSB-BP544836AVV
CSB-MP544836AVV
CSB-EP544836AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP544837BWU
CSB-EP544837BWU
CSB-BP544837BWU
CSB-MP544837BWU
CSB-EP544837BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus 5'-nucleotidase surE (surE) CSB-YP544838TMI
CSB-EP544838TMI
CSB-BP544838TMI
CSB-MP544838TMI
CSB-EP544838TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP544839FGA
CSB-EP544839FGA
CSB-BP544839FGA
CSB-MP544839FGA
CSB-EP544839FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP544840FGA
CSB-EP544840FGA
CSB-BP544840FGA
CSB-MP544840FGA
CSB-EP544840FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 5'-nucleotidase surE (surE) CSB-YP544841FGC
CSB-EP544841FGC
CSB-BP544841FGC
CSB-MP544841FGC
CSB-EP544841FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP544842FGC
CSB-EP544842FGC
CSB-BP544842FGC
CSB-MP544842FGC
CSB-EP544842FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP544843YAX
CSB-EP544843YAX
CSB-BP544843YAX
CSB-MP544843YAX
CSB-EP544843YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP544844YAX
CSB-EP544844YAX
CSB-BP544844YAX
CSB-MP544844YAX
CSB-EP544844YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP544845YAX
CSB-EP544845YAX
CSB-BP544845YAX
CSB-MP544845YAX
CSB-EP544845YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli GMP reductase (guaC) CSB-YP544846ENX
CSB-EP544846ENX
CSB-BP544846ENX
CSB-MP544846ENX
CSB-EP544846ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用资源

什么是重组蛋白?

重组蛋白是一种可操纵的蛋白质形式,可以通过多种方式产生大量蛋白质、修改基因序列和制造有用的商业产品。重组蛋白的技术资源在重组蛋白的开发过程中,除了表达系统之外,还面临着一系列令人困惑的选择,如表达载体的选择、表达蛋白的长度(全长或部分)、是否带标签等。在生产重组蛋白时,你必须做出很多决定。如果你选择的明智,你将获得高质量的重组蛋白,后续的实验更有可能成功。但是如果你做了错误的决定,你可能得不到你所需要的重组蛋白或者重组蛋白的质量和纯度不符合要求。在这里,来自CUSABIO的蛋白表达工程师根据他们开发重组蛋白的经验总结出了几个有用的提示,可以帮助你做出明智的决策。

第一条:重组蛋白的生产,在这篇文章中,我们旨在阐明重组蛋白的定义以及如何生产重组蛋白。

第二条:如何选择合适的表达载体?

表达载体是一种DNA分子,它携带特定的基因进入宿主细胞,并利用细胞的蛋白质合成机制来产生由该基因编码的蛋白质。表达载体可使外源基因在宿主细胞中高效表达,经过多年的发展,表达载体已成为一种成熟而受欢迎的生物技术。在重组蛋白生产过程中是一种基本成分。正如标题所示,本文主要总结了几个有用的技巧来选择合适的表达载体。

第三条:如何表达具有生物活性的蛋白质?

根据重组蛋白的不同应用,对其生物活性有不同的要求。但是我们如何确保蛋白质的产生具有生物活性呢?本文将围绕这个主题,并提供一些解决方案。除了以上文章,我们还收集了一些重组蛋白的常见问题。您可以单击以下链接查看。/technology-91.html。此外,我们还为我们的客户提供重组蛋白表达服务,您可以点击这里获取更多信息。注:如果您对CUSABIO的蛋白质有任何疑问,涉及到蛋白质的价格、交货、质量等,您可以点击橙色按钮为我们在线留言。

引用资料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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